sucrose catabolism in Escherichia coli BW25113

GapMind for catabolism of small carbon sources

sucrose catabolism in Escherichia coli BW25113

Best path

Also see fitness data for the top candidates

Rules

Overview: Sucrose utilization in GapMind is based on MetaCyc pathways sucrose degradation I via sucrose 6-phosphate hydrolase (link), pathway II via sucrose synthase (link), pathway III via invertase (link), and pathway IV via sucrose phosphorylase (link). Pathway V is similar to pathway III and is not reported in prokaryotes, so it is not included. There is no pathway VI. Pathway VII (via sucrose 3-dehydrogenase, link) is too poorly understood to include in GapMind.

all:

sucrose-PTS, scrB and scrK
or sucrose-transport, SUS, scrK, galU and pgmA
or sucrose-transport, ams, scrK and glk
or sucrose-transport, scrP, scrK and pgmA
or ams and glucose-utilization
or ams and fructose-utilization
Comment: In pathway I, a phosphotransferase system forms sucrose 6-phosphate, the hydrolyase scrB forms glucose-6-phosphate and fructose, and fructokinase forms fructose 6-phosphate. In pathway II, transport is followed by sucrose synthase (SUS) in reverse, forming fructose and UDP-glucose; the fructose is phosphorylated by scrK, while the UDP-glucose is transformed to glucose-6-phosphate by uridylyltransferase galU and phosphoglucomutase (pgmA). In pathway III, transport is followed by cleavage to glucose and fructose and phosphorylation of each. In pathway IV, transport is followed by phosphorylase scrP, producing fructose and glucose 1-phosphate, which are transformed by kinase scrK and phosphoglucomutase pgmA. Alternatively, sucrose can be hydrolyzed in the periplasm, followed by utilization of the glucose or fructose.

fructose-utilization:

fructose-PTS-6-phosphate
or fructose-transport and scrK
or fructose-PTS-1-phosphate, 1pfk, fba and tpi
Comment: For a PTS forming fructose 6-phosphate, no further steps are needed to reach central metabolism. For direct transport, the usual pathway is fructokinase (scrK), forming fructose 6-phosphate. For PTS forming fructose 1-phosphate, the usual path is phosphorylation (1pfk) and cleavage by fructose 1,6-bisphosphate aldolase (fba); triose-phosphate isomerase (tpi) converts the glycerone phosphate to D-glyceraldehyde 3-phosphate, which is a central metabolic intermediate.

fructose-PTS-1-phosphate:

fruA and fruB
or fruA and fruI
or fruA and fruD
or fruII-A, fruII-B and fruII-C
or fruII-ABC
Comment: Fructose 1-phosphate forming PTS systems contain FruA with either FruB, FruI, or FruD. FruA has EII-B'BC components; the other genes all have E-IIA but their domain content varies. FruB has E-IIA and Hpr components; FruI has EI-Hpr-IIA components; and FruD has E-IIA only.

fructose-transport:

araV, araU, araT and araS
or fruE, fruF, fruG and fruK
or frcA, frcB and frcC
or Slc2a5
or ffz
or glcP
or ght6
or STP6
or THT2A
or frt1
or fruP
or BT1758
Comment: Transporters and PTS systems (forming -1-phosphate or -6-phosphate) were found using query: transporter:fructose:D-fructose:BETA-D-FRUCTOSE.

fructose-PTS-6-phosphate:

levD, levE, levF and levG
or levDE, levF and levG

glucose-utilization:

glucose-transport and glk
or glucose-PTS
or gdh, gnl, gadh1, gadh2, gadh3, kguT, kguK, kguD, edd and eda
Comment: Glucose can be taken up and then phosphorylated to glucose 6-phosphate by the kinase glk. Or, both uptake and phosphorylation are catalyzed by a PTS system. Or, glucose is oxidized to glucono-1,5-lactone in the periplasm (by gdh), hydrolyzed to gluconate (by gnl), oxidized to 2-ketogluconate (by gadh123), taken up by kguT, phosphorylated to 2-dehydro-6-phosphogluconate (by kguK), reduced to gluconate 6-phosphate (by kguD), dehydrated by edd to 2-dehydro-3-deoxy-gluconate 6-phosphate, and cleaved by aldolase eda to pyruvate and D-glyceraldehyde 3-phosphate.

glucose-PTS:

ptsG-crr
or bglF
or ptsG and crr
or manX, manY and manZ

glucose-transport:

MFS-glucose
or SSS-glucose
or glcU'
or PAST-A
or SemiSWEET
or SWEET1
or mglA, mglB and mglC
or gtsA, gtsB, gtsC and gtsD
or glcS, glcT, glcU and glcV
or aglE', aglF', aglG' and aglK'
Comment: Transporters and PTS systems were analyzed uzing query: transporter:glucose:D-glucose:D-glucopyranose:ALPHA-GLUCOSE:GLC:CPD-15374

sucrose-PTS:

sacP
or ptsS
Comment: PTS form sucrose 6-phosphate.

sucrose-transport:

thuE, thuF, thuG and thuK
or aglE, aglF, aglG and aglK
or TMT1 and TMT2
or sut
or SLC45A2
or scrT
or sut1
or cscB
or SLC45A3
or SLC45A4
Comment: Transporters and PTS systems were analyzed using query: transporter:sucrose

101 steps (63 with candidates)

Or see definitions of steps

Step	Description	Best candidate	2nd candidate
ams	sucrose hydrolase (invertase)	b4239
fruA	fructose-specific PTS system (fructose 1-phosphate forming), EII-B'BC components	b2167	b0731
fruB	fructose-specific PTS system (fructose 1-phosphate forming), Hpr and EII-A components	b2169
1pfk	1-phosphofructokinase	b2168	b1723
fba	fructose 1,6-bisphosphate aldolase	b2925	b2097
tpi	triose-phosphate isomerase	b3919	b2926
Alternative steps:
aglE	sucrose ABC transporter, substrate-binding component AglK
aglE'	glucose ABC transporter, substrate-binding component (AglE)
aglF	sucrose ABC transporter, permease component 1 (AglF)
aglF'	glucose ABC transporter, permease component 1 (AglF)
aglG	sucrose ABC transporter, permease component 2 (AglG)	b4032
aglG'	glucose ABC transporter, permease component 2 (AglG)	b4032
aglK	sucrose ABC transporter, ATPase component AglK	b4035	b3450
aglK'	glucose ABC transporter, ATPase component (AglK)	b4035	b3450
araS	fructose ABC transporter, substrate-binding component AraS
araT	fructose ABC transporter, permease component 2 (AraT)
araU	fructose ABC transporter, permease component 1 (AraU)
araV	fructose ABC transporter, ATPase component AraV	b0262	b3450
bglF	glucose PTS, enzyme II (BCA components, BglF)	b3722	b2715
BT1758	fructose transporter
crr	glucose PTS, enzyme IIA	b2417	b0679
cscB	sucrose:H+ symporter CscB	b0343
eda	2-keto-3-deoxygluconate 6-phosphate aldolase	b1850	b4477
edd	phosphogluconate dehydratase	b1851	b3771
ffz	fructose facilitator (uniporter)
frcA	fructose ABC transporter, ATPase component FrcA	b3749	b1900
frcB	fructose ABC transporter, substrate-binding component FrcB
frcC	fructose ABC transporter, permease component FrcC	b3750	b2148
frt1	fructose:H+ symporter Frt1	b2943	b2841
fruD	fructose-specific PTS system (fructose 1-phosphate forming), EII-A component	b0731
fruE	fructose ABC transporter, substrate-binding component FruE	b4227
fruF	fructose ABC transporter, permease component 1 (FruF)	b4230	b3750
fruG	fructose ABC transporter, permease component 2 (FruG)	b4231	b3750
fruI	fructose-specific PTS system (fructose 1-phosphate forming), EI, Hpr, and EII-A components	b2416	b2829
fruII-A	fructose-specific PTS system (fructose 1-phosphate forming), EII-A component	b3204	b3947
fruII-ABC	fructose-specific PTS system (fructose 1-phosphate forming), EII-ABC components	b2167	b0731
fruII-B	fructose-specific PTS system (fructose 1-phosphate forming), EII-B component	b3950	b2167
fruII-C	fructose-specific PTS system (fructose 1-phosphate forming), EII-C component	b2167	b3949
fruK	fructose ABC transporter, ATPase component FruK	b4485	b3749
fruP	fructose porter FruP	b2801
gadh1	gluconate 2-dehydrogenase flavoprotein subunit
gadh2	gluconate 2-dehydrogenase cytochrome c subunit
gadh3	gluconate 2-dehydrogenase subunit 3
galU	glucose 1-phosphate uridylyltransferase	b1236	b2042
gdh	quinoprotein glucose dehydrogenase	b0124	b0837
ght6	high-affinity fructose transporter ght6
glcP	fructose:H+ symporter GlcP	b4031	b2943
glcS	glucose ABC transporter, substrate-binding component (GlcS)
glcT	glucose ABC transporter, permease component 1 (GlcT)
glcU	glucose ABC transporter, permease component 2 (GlcU)
glcU'	Glucose uptake protein GlcU
glcV	glucose ABC transporter, ATPase component (GclV)	b0262	b4035
glk	glucokinase	b2388	b0394
gnl	gluconolactonase
gtsA	glucose ABC transporter, substrate-binding component (GtsA)
gtsB	glucose ABC transporter, permease component 1 (GtsB)
gtsC	glucose ABC transporter, permease component 2 (GtsC)	b1312	b4032
gtsD	glucose ABC transporter, ATPase component (GtsD)	b4035	b3450
kguD	2-keto-6-phosphogluconate reductase	b3553	b2913
kguK	2-ketogluconokinase
kguT	2-ketogluconate transporter
levD	fructose PTS system (fructose 6-phosphate forming), EII-A component	b1817
levDE	fructose PTS system (fructose 6-phosphate forming), EII-AB component	b1817
levE	fructose PTS system (fructose 6-phosphate forming), EII-B component	b1817	b3133
levF	fructose PTS system (fructose 6-phosphate forming), EII-C component	b1818
levG	fructose PTS system (fructose 6-phosphate forming), EII-D component	b1819	b3140
manX	glucose PTS, enzyme EIIAB	b1817
manY	glucose PTS, enzyme EIIC	b1818
manZ	glucose PTS, enzyme EIID	b1819	b3140
MFS-glucose	glucose transporter, MFS superfamily	b2943	b2841
mglA	glucose ABC transporter, ATP-binding component (MglA)	b2149	b3567
mglB	glucose ABC transporter, substrate-binding component	b2150	b3566
mglC	glucose ABC transporter, permease component (MglC)	b2148	b3750
PAST-A	proton-associated sugar transporter A
pgmA	alpha-phosphoglucomutase	b0688	b2048
ptsG	glucose PTS, enzyme IICB	b1101	b0679
ptsG-crr	glucose PTS, enzyme II (CBA components, PtsG)	b0679	b1101
ptsS	sucrose phosphotransferase enzyme EII-BCA	b3722	b4240
sacP	sucrose phosphotransferase enzyme EII-BC	b4240	b3722
scrB	sucrose-6-phosphate hydrolase
scrK	fructokinase	b0394	b1119
scrP	sucrose phosphorylase	b1309
scrT	sucrose permease ScrT
SemiSWEET	Sugar transporter SemiSWEET
Slc2a5	fructose:H+ symporter	b2943	b2841
SLC45A2	sucrose transporter
SLC45A3	sucrose:H+ symporter
SLC45A4	sucrose:H+ symporter
SSS-glucose	Sodium/glucose cotransporter	b3679
STP6	sugar transport protein 6	b2943
SUS	sucrose synthase
sut	sucrose:proton symporter SUT/SUC
sut1	alpha-glucoside permease Sut1
SWEET1	bidirectional sugar transporter SWEET1
THT2A	fructose THT2A
thuE	sucrose ABC transporter, substrate-binding component ThuE
thuF	sucrose ABC transporter, permease component 1 (ThuF)	b1311	b3452
thuG	sucrose ABC transporter, permease component 2 (ThuG)	b1312	b4032
thuK	sucrose ABC transporter, ATPase component ThuK	b4035	b3450
TMT1	heteromeric sucrose:H+ symporter, TMT1 component
TMT2	heteromeric sucrose:H+ symporter, TMT2 component

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources