Aligments for a candidate for edd in Escherichia coli BW25113

GapMind for catabolism of small carbon sources

Aligments for a candidate for edd in Escherichia coli BW25113

Align Phosphogluconate dehydratase; 6-phosphogluconate dehydratase; EC 4.2.1.12 (characterized)
to candidate 18321 b4297 KpLE2 phage-like element; predicted dehydratase (NCBI)

Query= SwissProt::P21909
         (607 letters)



>FitnessBrowser__Keio:18321
          Length = 655

 Score =  188 bits (477), Expect = 7e-52
 Identities = 156/500 (31%), Positives = 243/500 (48%), Gaps = 38/500 (7%)

Query: 80  LSAHEPYYRYPEQMKVFAREVGAT--VQVAGGVPAMCDGVTQGQPGMEESLFSRDVIALA 137
           L+ H+ +Y    QMK  A  + A   +  A  V   CDG TQG  GM +SL  R+  ++ 
Sbjct: 83  LALHQGHYELDIQMKAAAEVIKANHALPYAVYVSDPCDGRTQGTTGMFDSLPYRNDASMV 142

Query: 138 TSVSLSHGMFEGAALLGI--CDKIVPGLLMGALRFGHLPTILVPSGPMTTGIPNKEKIRI 195
               L   + +  A++G+  CDK +P  +M      ++ T+LVP G        ++  ++
Sbjct: 143 MR-RLIRSLPDAKAVIGVASCDKGLPATMMALAAQHNIATVLVPGGATLPAKDGEDNGKV 201

Query: 196 RQLYAQGKIGQKELLDMEAA----CYHAEGTCTFYGTANTNQMVMEVLGLHMPGSAFVTP 251
           + + A+   G+  L D   A    C  + G C F GTA T+Q+V E LGL +P SA    
Sbjct: 202 QTIGARFANGELSLQDARRAGCKACASSGGGCQFLGTAGTSQVVAEGLGLAIPHSALAPS 261

Query: 252 GTPLRQALTRAAVHRVAELGWKGDDYRPLGKIIDEKSIVNAIVGLLATGGSTNHTMHIPA 311
           G P+ + + RA+      L  KG   R   +I+ +K+I NA+    A GGSTN  +HIPA
Sbjct: 262 GEPVWREIARASARAALNLSQKGITTR---EILTDKAIENAMTVHAAFGGSTNLLLHIPA 318

Query: 312 IARAAGV-IVNWNDFHDLSEVVPLIARIYPNGP---RDINEFQNAGGMAYVIKELLSANL 367
           IA  AG  I   +D+  +++ VP +  + PNGP     +N F  AGG+  V+  L S  L
Sbjct: 319 IAHQAGCHIPTVDDWIRINKRVPRLVSVLPNGPVYHPTVNAFM-AGGVPEVMLHLRSLGL 377

Query: 368 LNRDVTTIA----KGGIEEYAKAPALNDAGELVWKPAGEPGDDTILRPVSNPFAKDGGL- 422
           L+ DV T+     K  ++ +  +       +L+        D+ I+ P     AK  GL 
Sbjct: 378 LHEDVMTVTGSTLKENLDWWEHSERRQRFKQLLLDQEQINADEVIMSPQQ---AKARGLT 434

Query: 423 ---RLLEGNLG--RAMYKASAVDPK------FWTIEAPVRVFSDQDDVQKAFKAGELNKD 471
                  GN+    ++ K++A+DP        +  +   +V+  +       K  ++   
Sbjct: 435 STITFPVGNIAPEGSVIKSTAIDPSMIDEQGIYYHKGVAKVYLSEKSAIYDIKHDKIKAG 494

Query: 472 VIVVVRFQGPRANGMPELHKLTPALGVLQDNGYKVALVTDGRMSGATGKVPVALHVSPEA 531
            I+V+   GP   GM E +++T AL  L   G  V+L+TD R SG +    +  HV PEA
Sbjct: 495 DILVIIGVGPSGTGMEETYQVTSALKHL-SYGKHVSLITDARFSGVSTGACIG-HVGPEA 552

Query: 532 LGGGAIGKLRDGDIVRISVE 551
           L GG IGKLR GD++ I ++
Sbjct: 553 LAGGPIGKLRTGDLIEIKID 572


Lambda     K      H
   0.318    0.136    0.403 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 981
Number of extensions: 56
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 1
Length of query: 607
Length of database: 655
Length adjustment: 38
Effective length of query: 569
Effective length of database: 617
Effective search space:   351073
Effective search space used:   351073
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources