Align protein-Npi-phosphohistidine-sucrose phosphotransferase (EC 2.7.1.211) (characterized)
to candidate 17782 b3722 fused beta-glucoside-specific PTS enzymes: IIA component/IIB component/IIC component (NCBI)
Query= BRENDA::P12655 (664 letters) >FitnessBrowser__Keio:17782 Length = 625 Score = 313 bits (802), Expect = 2e-89 Identities = 219/666 (32%), Positives = 332/666 (49%), Gaps = 51/666 (7%) Query: 4 SKVASEVITAVG-KDNLVAAAHCATRLRLVLKDDSKVDQKALDKNADVKGTFKTDGQYQV 62 +++A +++ VG DN+V+ HCATRLR LKD+SK + L K + ++ GQ+QV Sbjct: 2 TELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLKKTPGIIMVVESGGQFQV 61 Query: 63 IIGPGDVNFVYDEIIKQTGLTEVSTDDLKKIAASGKKFNPIMALIKLLSDIFVPIIPALV 122 +IG V V+ + GL E + ++ + K N + + ++S IF P+I + Sbjct: 62 VIG-NHVADVFLAVNSVAGLDEKA----QQAPENDDKGNLLNRFVYVISGIFTPLIGLMA 116 Query: 123 AGGLLMALNNFLTSEGLFGTKSLVQQFPIIKGSSDMIQLMSAAPFWFLPILVGISAAKRF 182 A G+L +G+ Q G+ ++ S A FWF PI++G +A KRF Sbjct: 117 ATGIL---------KGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILGYTAGKRF 167 Query: 183 GANQFLGASIGMIMVAPGAANIIGLAANAPISKAATIGAYTGFWNIFGLHVTQASYTYQV 242 G N F IG +V P + L A KA +G + G+ VT +Y+ V Sbjct: 168 GGNPFTAMVIGGALVHP-----LILTAFENGQKADALGL-----DFLGIPVTLLNYSSSV 217 Query: 243 IPVLVAVWLLSILEKFFHKRLPSAVDFTFTPLLSVIITGFLTFIVIGPVMKEVSDWLTNG 302 IP++ + WL SILE+ + LPSA+ FTPLL +++ +TF+++GP+ +S+ + G Sbjct: 218 IPIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAAG 277 Query: 303 IVWLYDTTGFLGMGVFGALYSPVVMTGLHQSFPAIETQLISAFQNGTGHG-DFIFVTASM 361 +WLY V G + VM GLH + N T G D + Sbjct: 278 YLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPL------CINNFTVLGYDTMIPLLMP 331 Query: 362 ANVAQGAATFAIYFLTKDKKMKGLSSSSGVSALLGITEPALFGVNLKYRFPFFCALIGSA 421 A +AQ A ++ +D + K ++ S+ +++L GITEPA++GVNL ++PF A I A Sbjct: 332 AIMAQVGAALGVFLCERDAQKKVVAGSAALTSLFGITEPAVYGVNLPRKYPFVIACISGA 391 Query: 422 SAAAIAGLLQVVAVSLGSAGFLGFL-SIKASSIPFYVVCELISFAIAFAVTYGYGKTKAV 480 A I G Q S G F+ +I ++ I F V +I IA + G Sbjct: 392 LGATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAF-VGTVMLH 450 Query: 481 DVFA----AEAAVEEAIEEVQEIPEEAASAANKAQVTDEVLAAPLAGEAVELTSVNDPVF 536 + A A+ A +E EV PE+ + +P+ GE V L V D F Sbjct: 451 FITAKRQPAQGAPQEKTPEVITPPEQGG------------ICSPMTGEIVPLIHVADTTF 498 Query: 537 SSEAMGKGIAIKPSGNTVYAPVDGTVQIAFDTGHAYGIKSDNGAEILIHIGIDTVSMEGK 596 +S +GKGIAI PS V +PV G + F T HA GI+SD+G EILIH+GIDTV ++GK Sbjct: 499 ASGLLGKGIAILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGK 558 Query: 597 GFEQKVQADQKIKKGDVLGTFDSDKIAEAGLDNTTMFIVTNTADYASVETLASSGTVAVG 656 F V K+ GD L +FD I EAG D TT +++N+ D+ V + ++ G Sbjct: 559 FFSAHVNVGDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDV-LPHGTAQISAG 617 Query: 657 DSLLEV 662 + LL + Sbjct: 618 EPLLSI 623 Lambda K H 0.320 0.135 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1026 Number of extensions: 55 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 664 Length of database: 625 Length adjustment: 38 Effective length of query: 626 Effective length of database: 587 Effective search space: 367462 Effective search space used: 367462 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory