Align The sucrose porter, PtsS (regulated by SugR which also regulates other enzymes II) (characterized)
to candidate 18265 b4240 fused trehalose(maltose)-specific PTS enzyme: IIB component/IIC component (NCBI)
Query= TCDB::Q8NMD6 (661 letters) >FitnessBrowser__Keio:18265 Length = 473 Score = 252 bits (644), Expect = 2e-71 Identities = 137/448 (30%), Positives = 240/448 (53%), Gaps = 10/448 (2%) Query: 9 RILRDIGGEDNIVAAAHCATRLRLVLKDTKDVDRQSLDDDPDLKGTFETGGMFQIIVGPG 68 R++ +GG NI +HC TRLR VL + + ++ P +KG F G FQ+++G Sbjct: 12 RLIELVGGRGNIATVSHCITRLRFVLNQPANARPKEIEQLPMVKGCFTNAGQFQVVIGTN 71 Query: 69 DVDHVFKELDDATSKDIAVSTEQLKDVVANNANWFSRAVKVLADIFVPLIPILVGGGLLM 128 D+ + A++ V EQ+K +N W + + A IF PL+P L+ GGL++ Sbjct: 72 VGDYYQALI--ASTGQAQVDKEQVKKAARHNMKWHEQLISHFAVIFFPLLPALISGGLIL 129 Query: 129 AINNVLVAQDLFGPQSLVEMFPQISGVAEMINLMASAPFAFLPVLVGFTATKRFGGNEFL 188 NV+ + Q+L +M+P + + + + L+ A F +LPV + ++A K+ GG L Sbjct: 130 GFRNVIGDLPMSNGQTLAQMYPSLQTIYDFLWLIGEAIFFYLPVGICWSAVKKMGGTPIL 189 Query: 189 GAGIGMAMVFPSLVNGYDVAATMAAGEMP-MWSLFGLDVAQAGYQGTVLPVLVVSWILAT 247 G +G+ +V P L+N Y + ++P +W +A+ GYQ V+P L+ L Sbjct: 190 GIVLGVTLVSPQLMNAY-----LLGQQLPEVWDFGMFSIAKVGYQAQVIPALLAGLALGV 244 Query: 248 IEKFLHKRLKGTADFLITPVLTLLLTGFLTFIAIGPAMRWVGDVLAHGLQGLYDFG-GPV 306 IE L + + ++ PV +L+L FL IGP R +GD +A ++ L P+ Sbjct: 245 IETRLKRIVPDYLYLVVVPVCSLILAVFLAHALIGPFGRMIGDGVAFAVRHLMTGSFAPI 304 Query: 307 GGLLFGLVYSPIVITGLHQSFPPIELELF-NQGGSFIFATASMANIAQGAACLAVFFLAK 365 G LFG +Y+P+VITG+HQ+ I+L++ + GG+ ++ +++NIAQG+A + + ++ Sbjct: 305 GAALFGFLYAPLVITGVHQTTLAIDLQMIQSMGGTPVWPLIALSNIAQGSAVIGIIISSR 364 Query: 366 SEKLKGLAGASGVSAVLGITEPAIFGVNLRLRWPFFIGIGTAAIGGALIALFNIKAVALG 425 + ++ + +SA LG+TEPA++G+NL+ R+P + + + G L L + A +G Sbjct: 365 KHNEREISVPAAISAWLGVTEPAMYGINLKYRFPMLCAMIGSGLAGLLCGLNGVMANGIG 424 Query: 426 AAGFLGVVSIDAPDMVMFLVCAVVTFFI 453 G G++SI +F + + I Sbjct: 425 VGGLPGILSIQPSYWQVFALAMAIAIII 452 Lambda K H 0.322 0.139 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 669 Number of extensions: 33 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 661 Length of database: 473 Length adjustment: 36 Effective length of query: 625 Effective length of database: 437 Effective search space: 273125 Effective search space used: 273125 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory