GapMind for catabolism of small carbon sources

 

thymidine catabolism in Escherichia coli BW25113

Best path

nupG, deoA, deoB, deoC, adh, ackA, pta

Also see fitness data for the top candidates

Rules

Overview: Thymidine degradation in GapMind is based on thymidine phoshorylase (EC 2.4.2.4), which yields 2-deoxyribose-1-phosphate and thymine. The catabolism of thymine is not represented, as it may be excreted.

12 steps (11 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
nupG thymidine permease NupG/XapB b2964 b2406
deoA thymidine phosphorylase DeoA b4382 b3831
deoB phosphopentomutase b4383 b3380
deoC deoxyribose-5-phosphate aldolase b4381
adh acetaldehyde dehydrogenase (not acylating) b1746 b1241
ackA acetate kinase b3115 b2296
pta phosphate acetyltransferase b2297 b2458
Alternative steps:
acs acetyl-CoA synthetase, AMP-forming b4069 b0335
ald-dh-CoA acetaldehyde dehydrogenase, acylating b1241 b2455
nupC thymidine permease NupC b2393 b2161
Slc28a3 thymidine:Na+ symporter SLC28A3 b2164 b2161
Slc29a1 thymidine transporter Slc29a1

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory