Aligments for a candidate for treB in Escherichia coli BW25113

GapMind for catabolism of small carbon sources

Aligments for a candidate for treB in Escherichia coli BW25113

Align Trehalose permease IIC protein (characterized, see rationale)
to candidate 17782 b3722 fused beta-glucoside-specific PTS enzymes: IIA component/IIB component/IIC component (NCBI)

Query= uniprot:A0A1N7UR85
         (480 letters)



>lcl|FitnessBrowser__Keio:17782 b3722 fused beta-glucoside-specific
           PTS enzymes: IIA component/IIB component/IIC component
           (NCBI)
          Length = 625

 Score =  220 bits (560), Expect = 1e-61
 Identities = 141/465 (30%), Positives = 241/465 (51%), Gaps = 29/465 (6%)

Query: 8   IAREILENLGGSDNLEQAAHCVTRLRLALKDPSLVNSSALNQVDLVKGSFFTGGLFQVVI 67
           +AR+I+  +GG+DN+    HC TRLR  LKD S   +  L +   +     +GG FQVVI
Sbjct: 4   LARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLKKTPGIIMVVESGGQFQVVI 63

Query: 68  GPGEVEKVYAALREQTGLAAATIADVKKKGADKTNAMQRLVRVFSDVFMPILPALIIAGL 127
           G   V  V+ A+    GL     A    +  DK N + R V V S +F P++  +   G+
Sbjct: 64  G-NHVADVFLAVNSVAGLDEK--AQQAPENDDKGNLLNRFVYVISGIFTPLIGLMAATGI 120

Query: 128 LMGVNNLMGAQGMFIEGKTLLEAYPNLDGLWSLINLMANTSFVFLPALVGWSAAKRFGGS 187
           L G    M A  +  +  T         G + ++   ++  F F P ++G++A KRFGG+
Sbjct: 121 LKG----MLALALTFQWTT------EQSGTYLILFSASDALFWFFPIILGYTAGKRFGGN 170

Query: 188 EILGIVLGLMLVHPDLLNAWNYGKAV--AGLDGQSLPYFDIFGWFKIEKVGYQGQILPIL 245
               +V+G  LVHP +L A+  G+     GLD   +P         +  + Y   ++PI+
Sbjct: 171 PFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIP---------VTLLNYSSSVIPII 221

Query: 246 MAAYVMSVIEKGLRARVPNAIQLLVVPITTIVVTGVLALAIIGPVTRHLGILITEGVVTL 305
            +A++ S++E+ L A +P+AI+    P+  ++V   +   ++GP++  +  LI  G + L
Sbjct: 222 FSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAAGYLWL 281

Query: 306 FDLAPMVGGAIFGLLYAPLVITGMHHMFLAVDLQLISTQGGTFIWPMIVMSNLAQGSAAL 365
           +   P   GA+ G  +   V+ G+H   + + +   +  G   + P+++ + +AQ  AAL
Sbjct: 282 YQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIMAQVGAAL 341

Query: 366 AVFYTTRNARDKSMASTSAISAYFGITEPAMFGVNLRFKFPFYAALVGSALGSIFLALNK 425
            VF   R+A+ K +A ++A+++ FGITEPA++GVNL  K+PF  A +  ALG+  +   +
Sbjct: 342 GVFLCERDAQKKVVAGSAALTSLFGITEPAVYGVNLPRKYPFVIACISGALGATIIGYAQ 401

Query: 426 VQASAIGVGGLPGFISIVPQ-----SIAVFVIGMVIAMVVPFVLT 465
            +  + G+  +  F+  +P      ++   VIG VIA+   FV T
Sbjct: 402 TKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGT 446


Lambda     K      H
   0.325    0.141    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 761
Number of extensions: 42
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 480
Length of database: 625
Length adjustment: 36
Effective length of query: 444
Effective length of database: 589
Effective search space:   261516
Effective search space used:   261516
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources