GapMind for catabolism of small carbon sources

 

Aligments for a candidate for Ac3H11_1694 in Escherichia coli BW25113

Align ABC transporter ATP-binding protein (characterized, see rationale)
to candidate 17517 b3456 leucine/isoleucine/valine transporter subunit (NCBI)

Query= uniprot:A0A165KER0
         (358 letters)



>lcl|FitnessBrowser__Keio:17517 b3456 leucine/isoleucine/valine
           transporter subunit (NCBI)
          Length = 425

 Score =  236 bits (603), Expect = 6e-67
 Identities = 146/347 (42%), Positives = 200/347 (57%), Gaps = 46/347 (13%)

Query: 13  VALLVLPLILQSF-GNAWVRIADLALLYVLLALGLNIVVGYAGLLDLGYVAFYAVGAYLF 71
           VALLVL +          V IA L ++Y++L LGLN+VVG +GLL LGY  FYA+GAY F
Sbjct: 94  VALLVLAVAWPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTF 153

Query: 72  ALMASPHLADNFAAFAAMFPNGLHTSLWIVIPVAALLAAFFGAMLGAPTLKLRGDYLAIV 131
           AL+   +              GL    W  +P+A L+AA  G +LG P L+LRGDYLAIV
Sbjct: 154 ALLNHYY--------------GL--GFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIV 197

Query: 132 TLGFGEIIRIFLNNLDHPVNLTNGPKGLGQIDSVKVFGLDLGKRLEVFGFDINS------ 185
           TLGFGEI+RI L N      +T GP G+ QI    +FGL+  +     G+D  S      
Sbjct: 198 TLGFGEIVRILLLN---NTEITGGPNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLK 254

Query: 186 ------VTLYYYLFLVLVVVSVIICYRLQDSRIGRAWMAIREDEIAAKAMGINTRNMKLL 239
                 V   Y + L+LVV+S+ +  RL    +GRAW A+REDEIA +++G++ R +KL 
Sbjct: 255 YDPSDRVIFLYLVALLLVVLSLFVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLT 314

Query: 240 AFGMGASFGGVSGAMFGAFQGFVSPESFSLMESVMIVAMVVLGGIGHIPGVILGAVLLSA 299
           AF + A+F G +G +F A QGFVSPESF+  ES  ++A+VVLGG+G    VIL A+LL  
Sbjct: 315 AFTISAAFAGFAGTLFAARQGFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVV 374

Query: 300 LPEVLRYVAGPLQAMTDGRLDSAILRQLLIALAMIIIMLLRPRGLWP 346
             E++R              D      L++   M+++M+ RP+GL P
Sbjct: 375 SRELMR--------------DFNEYSMLMLGGLMVLMMIWRPQGLLP 407


Lambda     K      H
   0.328    0.144    0.430 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 362
Number of extensions: 19
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 358
Length of database: 425
Length adjustment: 31
Effective length of query: 327
Effective length of database: 394
Effective search space:   128838
Effective search space used:   128838
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory