GapMind for catabolism of small carbon sources

 

Aligments for a candidate for acdH in Escherichia coli BW25113

Align 2-methylbutanoyl-CoA dehydrogenase / butanoyl-CoA dehydrogenase / isobutyryl-CoA dehydrogenase (EC 1.3.8.1; EC 1.3.8.5) (characterized)
to candidate 14185 b0039 crotonobetaine reductase subunit II, FAD-binding (NCBI)

Query= reanno::pseudo3_N2E3:AO353_25680
         (375 letters)



>lcl|FitnessBrowser__Keio:14185 b0039 crotonobetaine reductase
           subunit II, FAD-binding (NCBI)
          Length = 380

 Score =  180 bits (457), Expect = 5e-50
 Identities = 116/373 (31%), Positives = 187/373 (50%), Gaps = 5/373 (1%)

Query: 2   LPTDEQLQISDAARQFAQERLKPFAAEWDREHRFPKEAIGEMAELGFFGMLVPEQWGGCD 61
           L  +++L ++      A E  + + AE DR+  +P+  +  +A++G   +L+PE+ GG D
Sbjct: 5   LNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVKALADMGIDSLLIPEEHGGLD 64

Query: 62  TGYLAYAMALEEIAAGDGACSTIMSVHNSVGCVPILKFGNDDQKERFLKPLASGAMLGAF 121
            G++  A    E+  G     T +      G    L+ G  +Q ++ +    +G  +   
Sbjct: 65  AGFVTLAAVWMEL--GRLGAPTYVLYQLPGGFNTFLREGTQEQIDKIMAFRGTGKQMWNS 122

Query: 122 ALTEPQAGSDASSLKTR-ARLNGDHYVLNGCKQFITSGQNAGVVIVFAVTDPSAGKRGIS 180
           A+TEP AGSD  SLKT   R NG  Y LNG K FITS      ++V A    S  K   +
Sbjct: 123 AITEPGAGSDVGSLKTTYTRRNGKIY-LNGSKCFITSSAYTPYIVVMARDGASPDKPVYT 181

Query: 181 AFIVPTDSPGYKVARVEDKLGQHASDTCQILFEDVQVPVANRLGEEGEGYKIALANLEGG 240
            + V    PG KV ++E KLG      C+I F+DV++   +  G EG G+       +  
Sbjct: 182 EWFVDMSKPGIKVTKLE-KLGLRMDSCCEITFDDVELDEKDMFGREGNGFNRVKEEFDHE 240

Query: 241 RVGIASQSVGMARAAFEAARDYARERESFGKPIIEHQAVAFRLADMATQIAVARQMVHYA 300
           R  +A  + G A  AFE A  YA +R  FG+ I   Q +  + A MA ++   + M++ A
Sbjct: 241 RFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRFQLIQEKFAHMAIKLNSMKNMLYEA 300

Query: 301 AALRDSGKPALVEASMAKLFASEMAEKVCSTALQTLGGYGYLSDFPLERIYRDVRVCQIY 360
           A   D+G     +A+M K F +  A +V  +A+Q LGG G   +  + R +RD+RV ++ 
Sbjct: 301 AWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQVLGGVGIAGNHRISRFWRDLRVDRVS 360

Query: 361 EGTSDIQRMVISR 373
            G+ ++Q + + R
Sbjct: 361 GGSDEMQILTLGR 373


Lambda     K      H
   0.319    0.134    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 300
Number of extensions: 9
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 375
Length of database: 380
Length adjustment: 30
Effective length of query: 345
Effective length of database: 350
Effective search space:   120750
Effective search space used:   120750
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory