GapMind for catabolism of small carbon sources

 

D-xylose catabolism in Escherichia coli BW25113

Best path

xylF, xylG, xylH, xylA, xylB

Also see fitness data for the top candidates

Rules

Overview: Xylose degradation in GapMind is based on MetaCyc pathways I via D-xylulose (link), II via xylitol (link), III or V via 2-dehydro-3-deoxy-D-arabinonate (DKDP) dehydratase (link, link), IV via DKDP aldolase (link), as well as another pathway via DKDP dehydrogenase (PMC6336799).

36 steps (23 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
xylF ABC transporter for xylose, substrate binding component xylF b3566
xylG ABC transporter for xylose, ATP-binding component xylG b3567 b2149
xylH ABC transporter for xylose, permease component xylH b3568 b3750
xylA xylose isomerase b3565
xylB xylulokinase b3564
Alternative steps:
aldA (glycol)aldehyde dehydrogenase b1415 b2661
aldox-large (glycol)aldehyde oxidoreductase, large subunit
aldox-med (glycol)aldehyde oxidoreductase, medium subunit
aldox-small (glycol)aldehyde oxidoreductase, small subunit b0286 b2868
araS component of Arabinose, fructose, xylose porter
araT component of Arabinose, fructose, xylose porter
araU component of Arabinose, fructose, xylose porter
araV component of Arabinose, fructose, xylose porter b0262 b3450
DKDP-aldolase 2-dehydro-3-deoxy-D-arabinonate aldolase b0268 b4298
DKDP-dehydrog D-2-keto-3-deoxypentoate dehydrogenase b2426 b1093
dopDH 2,5-dioxopentanonate dehydrogenase b2661 b0312
Echvi_1871 sodium/xylose cotransporter b3679
gal2 galactose/glucose/xylose uniporter
glcB malate synthase b2976 b4014
glcP glucose/mannose/xylose:H+ symporter
gtsA xylose ABC transporter, periplasmic substrate-binding component GtsA
gtsB xylose ABC transporter, permease component 1 GtsB
gtsC xylose ABC transporter, permease component 2 GtsC
gtsD xylose ABC transporter, ATPase component GtsD b3450 b4035
gyaR glyoxylate reductase b3553 b2913
HDOP-hydrol 5-hydroxy-2,4-dioxopentanonate hydrolase b1180
kdaD 2-keto-3-deoxy-D-arabinonate dehydratase
xad D-xylonate dehydratase b0269 b4297
xdh D-xylose dehydrogenase b4266 b1093
xdhA xylitol dehydrogenase b1774 b1776
xylC xylonolactonase
xylE_Tm ABC transporter for xylose, substrate binding component xylE
xylF_Tm ABC transporter for xylose, permease component xylF b3750 b2546
xylK_Tm ABC transporter for xylose, ATP binding component xylK b3749 b2149
xylT D-xylose transporter b4031 b2841
xyrA xylitol reductase b3012 b1771

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory