Protein Ga0059261_0562 in Sphingomonas koreensis DSMZ 15582
Annotation: FitnessBrowser__Korea:Ga0059261_0562
Length: 232 amino acids
Source: Korea in FitnessBrowser
Candidate for 33 steps in catabolism of small carbon sources
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|
| L-glutamate catabolism | gltL | med | GluA aka CGL1950, component of Glutamate porter (characterized) | 40% | 83% | 136 | Uncharacterized ABC transporter ATP-binding protein Rv0986 | 41% | 170.6 |
| L-histidine catabolism | PA5503 | lo | Methionine import ATP-binding protein MetN 2, component of L-Histidine uptake porter, MetIQN (characterized) | 41% | 63% | 147.1 | Uncharacterized ABC transporter ATP-binding protein Rv0986 | 41% | 170.6 |
| D-glucosamine (chitosamine) catabolism | AO353_21725 | lo | ABC transporter for D-Glucosamine, putative ATPase component (characterized) | 38% | 83% | 132.1 | Uncharacterized ABC transporter ATP-binding protein Rv0986 | 41% | 170.6 |
| L-asparagine catabolism | glnQ | lo | Glutamine ABC transporter ATP-binding protein, component of Glutamine transporter, GlnQP. Takes up glutamine, asparagine and glutamate which compete for each other for binding both substrate and the transmembrane protein constituent of the system (Fulyani et al. 2015). Tandem substrate binding domains (SBDs) differ in substrate specificity and affinity, allowing cells to efficiently accumulate different amino acids via a single ABC transporter. Analysis revealed the roles of individual residues in determining the substrate affinity (characterized) | 39% | 83% | 131 | Uncharacterized ABC transporter ATP-binding protein Rv0986 | 41% | 170.6 |
| L-arginine catabolism | artP | lo | BgtA aka SLR1735, component of Arginine/lysine/histidine/glutamine porter (characterized) | 40% | 81% | 129.4 | Uncharacterized ABC transporter ATP-binding protein Rv0986 | 41% | 170.6 |
| L-histidine catabolism | bgtA | lo | BgtA aka SLR1735, component of Arginine/lysine/histidine/glutamine porter (characterized) | 40% | 81% | 129.4 | Uncharacterized ABC transporter ATP-binding protein Rv0986 | 41% | 170.6 |
| L-lysine catabolism | hisP | lo | BgtA aka SLR1735, component of Arginine/lysine/histidine/glutamine porter (characterized) | 40% | 81% | 129.4 | Uncharacterized ABC transporter ATP-binding protein Rv0986 | 41% | 170.6 |
| L-asparagine catabolism | peb1C | lo | PEB1C, component of Uptake system for glutamate and aspartate (characterized) | 36% | 90% | 127.1 | Uncharacterized ABC transporter ATP-binding protein Rv0986 | 41% | 170.6 |
| L-aspartate catabolism | peb1C | lo | PEB1C, component of Uptake system for glutamate and aspartate (characterized) | 36% | 90% | 127.1 | Uncharacterized ABC transporter ATP-binding protein Rv0986 | 41% | 170.6 |
| D-sorbitol (glucitol) catabolism | mtlK | lo | ABC transporter for D-Sorbitol, ATPase component (characterized) | 36% | 56% | 126.7 | Uncharacterized ABC transporter ATP-binding protein Rv0986 | 41% | 170.6 |
| N-acetyl-D-glucosamine catabolism | SMc02869 | lo | N-Acetyl-D-glucosamine ABC transport system, ATPase component (characterized) | 38% | 61% | 123.6 | Uncharacterized ABC transporter ATP-binding protein Rv0986 | 41% | 170.6 |
| D-glucosamine (chitosamine) catabolism | SMc02869 | lo | N-Acetyl-D-glucosamine ABC transport system, ATPase component (characterized) | 38% | 61% | 123.6 | Uncharacterized ABC transporter ATP-binding protein Rv0986 | 41% | 170.6 |
| lactose catabolism | lacK | lo | ABC transporter for Lactose, ATPase component (characterized) | 35% | 55% | 123.2 | Uncharacterized ABC transporter ATP-binding protein Rv0986 | 41% | 170.6 |
| D-cellobiose catabolism | gtsD | lo | ABC transporter for D-Glucose-6-Phosphate, ATPase component (characterized) | 36% | 55% | 119.8 | Uncharacterized ABC transporter ATP-binding protein Rv0986 | 41% | 170.6 |
| D-glucose catabolism | gtsD | lo | ABC transporter for D-Glucose-6-Phosphate, ATPase component (characterized) | 36% | 55% | 119.8 | Uncharacterized ABC transporter ATP-binding protein Rv0986 | 41% | 170.6 |
| lactose catabolism | gtsD | lo | ABC transporter for D-Glucose-6-Phosphate, ATPase component (characterized) | 36% | 55% | 119.8 | Uncharacterized ABC transporter ATP-binding protein Rv0986 | 41% | 170.6 |
| D-maltose catabolism | gtsD | lo | ABC transporter for D-Glucose-6-Phosphate, ATPase component (characterized) | 36% | 55% | 119.8 | Uncharacterized ABC transporter ATP-binding protein Rv0986 | 41% | 170.6 |
| sucrose catabolism | gtsD | lo | ABC transporter for D-Glucose-6-Phosphate, ATPase component (characterized) | 36% | 55% | 119.8 | Uncharacterized ABC transporter ATP-binding protein Rv0986 | 41% | 170.6 |
| trehalose catabolism | gtsD | lo | ABC transporter for D-Glucose-6-Phosphate, ATPase component (characterized) | 36% | 55% | 119.8 | Uncharacterized ABC transporter ATP-binding protein Rv0986 | 41% | 170.6 |
| D-xylose catabolism | gtsD | lo | ABC transporter for D-Glucose-6-Phosphate, ATPase component (characterized) | 36% | 55% | 119.8 | Uncharacterized ABC transporter ATP-binding protein Rv0986 | 41% | 170.6 |
| L-histidine catabolism | Ac3H11_2560 | lo | ABC transporter for L-Histidine, ATPase component (characterized) | 40% | 68% | 118.2 | Uncharacterized ABC transporter ATP-binding protein Rv0986 | 41% | 170.6 |
| D-galactose catabolism | PfGW456L13_1897 | lo | ABC transporter for D-Galactose and D-Glucose, ATPase component (characterized) | 37% | 55% | 116.3 | Uncharacterized ABC transporter ATP-binding protein Rv0986 | 41% | 170.6 |
| D-maltose catabolism | aglK | lo | ABC transporter for D-Maltose and D-Trehalose, ATPase component (characterized) | 33% | 55% | 116.3 | Uncharacterized ABC transporter ATP-binding protein Rv0986 | 41% | 170.6 |
| D-maltose catabolism | thuK | lo | ABC transporter for D-Maltose and D-Trehalose, ATPase component (characterized) | 33% | 55% | 116.3 | Uncharacterized ABC transporter ATP-binding protein Rv0986 | 41% | 170.6 |
| sucrose catabolism | aglK | lo | ABC transporter for D-Maltose and D-Trehalose, ATPase component (characterized) | 33% | 55% | 116.3 | Uncharacterized ABC transporter ATP-binding protein Rv0986 | 41% | 170.6 |
| trehalose catabolism | aglK | lo | ABC transporter for D-Maltose and D-Trehalose, ATPase component (characterized) | 33% | 55% | 116.3 | Uncharacterized ABC transporter ATP-binding protein Rv0986 | 41% | 170.6 |
| D-maltose catabolism | malK | lo | Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 (characterized) | 32% | 56% | 112.8 | Uncharacterized ABC transporter ATP-binding protein Rv0986 | 41% | 170.6 |
| D-cellobiose catabolism | aglK' | lo | Maltose/maltodextrin import ATP-binding protein; EC 3.6.3.19 (characterized, see rationale) | 34% | 53% | 112.5 | Uncharacterized ABC transporter ATP-binding protein Rv0986 | 41% | 170.6 |
| D-glucose catabolism | aglK' | lo | Maltose/maltodextrin import ATP-binding protein; EC 3.6.3.19 (characterized, see rationale) | 34% | 53% | 112.5 | Uncharacterized ABC transporter ATP-binding protein Rv0986 | 41% | 170.6 |
| lactose catabolism | aglK' | lo | Maltose/maltodextrin import ATP-binding protein; EC 3.6.3.19 (characterized, see rationale) | 34% | 53% | 112.5 | Uncharacterized ABC transporter ATP-binding protein Rv0986 | 41% | 170.6 |
| D-maltose catabolism | aglK' | lo | Maltose/maltodextrin import ATP-binding protein; EC 3.6.3.19 (characterized, see rationale) | 34% | 53% | 112.5 | Uncharacterized ABC transporter ATP-binding protein Rv0986 | 41% | 170.6 |
| sucrose catabolism | aglK' | lo | Maltose/maltodextrin import ATP-binding protein; EC 3.6.3.19 (characterized, see rationale) | 34% | 53% | 112.5 | Uncharacterized ABC transporter ATP-binding protein Rv0986 | 41% | 170.6 |
| trehalose catabolism | aglK' | lo | Maltose/maltodextrin import ATP-binding protein; EC 3.6.3.19 (characterized, see rationale) | 34% | 53% | 112.5 | Uncharacterized ABC transporter ATP-binding protein Rv0986 | 41% | 170.6 |
Sequence Analysis Tools
View Ga0059261_0562 at FitnessBrowser
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
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Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
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Sequence
MSIDPAADIAIRARNVTLTLGTREAPTEILKGIDVDIARGSSVAILGPSGSGKSSLMAIL
SGLERASGGEVSVAGIAYGTLDEDGLARARRGRVGIVLQAFHLLPTMTAHENVAVPLELA
GAPDAFARAGAELDAVGLGHRLTHYPVQLSGGEQQRVAIARAVAGRPEILFADEPTGNLD
GATSGAIVDLLFDRQRAADATLLIITHDPALAERCDRVLTMRDGLIVADSAA
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory