Alignments for a candidate for bgl in Sphingomonas koreensis DSMZ 15582

GapMind for catabolism of small carbon sources

Alignments for a candidate for bgl in Sphingomonas koreensis DSMZ 15582

Align β-glucosidase (SalB) (EC 3.2.1.21) (characterized)
to candidate Ga0059261_0499 Ga0059261_0499 Beta-glucosidase-related glycosidases

Query= CAZy::AAF21799.1
         (649 letters)



>FitnessBrowser__Korea:Ga0059261_0499
          Length = 622

 Score =  615 bits (1587), Expect = e-180
 Identities = 313/590 (53%), Positives = 397/590 (67%), Gaps = 5/590 (0%)

Query: 41  SFRDLNRDGTLNPYEDWRLSPEVRAADLVARMTLAEKAGAGVHGTAPIQGGPM-ASGPAY 99
           ++RDLNR+G ++PYE+  L  + R  DL+ARMT  EKAG  +HGT P  G  + AS   Y
Sbjct: 14  AYRDLNRNGVMDPYENPALPVDARVDDLLARMTPEEKAGQLLHGTLPGHGSAIGASTSGY 73

Query: 100 DMTAAQAIIRDQHLNSLITRMAIAPADFAAENNRLQGIAAGTRLGIPLTISTDPRNHFQV 159
           D+  A+A+I DQH++S ITR+ + PA FA +NN +Q +A  TRLGIP TISTDPR+HF  
Sbjct: 74  DLDKARAMIADQHVSSFITRLVMPPAQFAEQNNAVQKLAEATRLGIPATISTDPRHHFHA 133

Query: 160 LGGASVAASGFSQWPETLGFGALNDPALTRRFADLVRAEYRAVGIQMALSPQADLATEPR 219
             GAS    GFSQWPETL F A+ DPAL RRF +  R EYRAVGI MALSPQAD+ +EPR
Sbjct: 134 TAGASTHGGGFSQWPETLAFAAIGDPALVRRFGEAARREYRAVGIHMALSPQADIGSEPR 193

Query: 220 WSRINGTFGEDPARVSAQVKAYVQGMQGADTGLAPGGVATVVKHWVGYGAQIDGYDGHNY 279
           W RI  TFG DPA VS    AYV+G QG  +G+   GVATVVKHWVGYGA  +G+DGHN+
Sbjct: 194 WPRITATFGSDPATVSRLAGAYVEGFQGGPSGVTRDGVATVVKHWVGYGAAPEGHDGHNF 253

Query: 280 YGRFTDFTKGGFDRHVAAFQGAFEAGATGIMPTYTIQKGLSLEGKPVEPVSGGYNKQMLI 339
           YGR    T   F++HVAAF GAF   + G+MPTY I +G  L+G  +EPV  G++KQ+L 
Sbjct: 254 YGRAVSMTDAEFEKHVAAFDGAFAVRSAGVMPTYVIVRGPRLKGAALEPVGAGFSKQLLN 313

Query: 340 DLLRGTHKFKGLILSDWAITNDCNESCRT--GNPPQQPKDIATPWGVEDLTQPQRFAKGM 397
           DLLR    + GLI+SDWAIT DC+ +C     + PQ P  I  PWGVE L+  +RFAKG+
Sbjct: 314 DLLRREKGYGGLIISDWAITRDCDAACTAPDASNPQLPGSIGMPWGVEHLSVTERFAKGL 373

Query: 398 LAGIDQFGGVNDGLPLLAAVEQKLLPEARLNEAVATIMTLKFEQGLFENPFVDPAAAATI 457
            AG+DQFGGV+D  P+LA + +  +  AR++ +V  I+ LKFE GLFENPFVDPA A  +
Sbjct: 374 EAGLDQFGGVDDPAPILAVMREGKVDAARIDASVRRILRLKFELGLFENPFVDPARAEAV 433

Query: 458 VGRADVVAEGRATQAKSLVMLENRLGPAPLPAGGGKRLFIYGVDAANAKAAGFTIAASLD 517
           +G   V A+  A Q    V+L N+   A LP   G RL+++GVDA  A+AAGF +    +
Sbjct: 434 LGDPGVRAQADAAQRAGQVLLRNQ--GALLPLRRGARLWLHGVDADAARAAGFQVVGKPE 491

Query: 518 EADIALIRLKAPFQTLHPGFFFGRMQHEGDLDFKEGDAGLTLVRQAAAKVPVILTIYLDR 577
           EAD AL+R   PF+TLHP  FFG  QHEG LDF+  DA    +  AA  VPVI+ + +DR
Sbjct: 492 EADAALVRTATPFETLHPHHFFGSRQHEGRLDFRPDDADTKTIAAAARHVPVIVAVEMDR 551

Query: 578 PAILTNIKPHAATLIGEFGITDAALFDALTGKVAPMGKLPFELPATMAAV 627
           PAILT + PH+  L+  FG +DAAL D + G   P GKLPF+LP +M  V
Sbjct: 552 PAILTALLPHSRALLVTFGASDAALLDVVQGVAKPRGKLPFDLPRSMEDV 601


Lambda     K      H
   0.319    0.137    0.408 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1210
Number of extensions: 71
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 649
Length of database: 622
Length adjustment: 38
Effective length of query: 611
Effective length of database: 584
Effective search space:   356824
Effective search space used:   356824
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources