Align coniferin β-glucosidase (Cbg1;Cbg-1) (EC 3.2.1.21|3.2.1.126) (characterized)
to candidate Ga0059261_0769 Ga0059261_0769 Beta-glucosidase-related glycosidases
Query= CAZy::AAA22082.1 (818 letters) >FitnessBrowser__Korea:Ga0059261_0769 Length = 838 Score = 531 bits (1369), Expect = e-155 Identities = 312/822 (37%), Positives = 448/822 (54%), Gaps = 35/822 (4%) Query: 2 IDDILDKMTLEEQVSLLSGADFWTTVAIERLGVPKIKVTDGPNGARGGGSLVGGVKSACF 61 + D++ ++TL+E++ LL+G I RLG+P +K+TDGP G R G + F Sbjct: 37 VADLMARLTLDEKILLLAGESSMALNPIPRLGIPAVKMTDGPTGVRSPD----GKPATVF 92 Query: 62 PVAIALGATWDPELIERAGVALGGQAKSKGASVLLAPTVNIHRSGLNGRNFECYSEDPAL 121 PV +AL A+W+P+L G A+ + K+ GA VLLAPTVNI R+ GRNFE YSEDP L Sbjct: 93 PVGVALAASWNPDLAATVGAAVARETKAHGADVLLAPTVNIVRTPRWGRNFETYSEDPWL 152 Query: 122 TAACAVAYINGVQSQGVAATIKHFVANESEIERQTMSSDVDERTLREIYLPPFEEAVKKA 181 + + Y+ GVQ +G+ ++KHF N E R + S VD+RTL EIYLP FE V++A Sbjct: 153 SGRMGLGYVRGVQGEGIGVSLKHFAVNNQESYRFVVDSIVDQRTLHEIYLPAFEMVVREA 212 Query: 182 GVKAVMSSYNKLNGTYTSENPWLLTKVLREEWGFDGVVMSDWFGSHSTAETINAGLDLEM 241 +VM+SYNK+NGTY +EN WLLT +L++EWG+ G V+SDW +H+TAE +NAG+DLEM Sbjct: 213 DPWSVMASYNKINGTYAAENRWLLTDLLKKEWGYRGFVVSDWGATHTTAEAVNAGMDLEM 272 Query: 242 PGPWRDRGEKLVAAVREGKVKAETVRASARRILLLLERVGAFEKAPDLAEHALDLPEDRA 301 PGP + G+KL AAV EGKV + +ARR++ L+ R G E+ E A L + A Sbjct: 273 PGPPKHFGDKLKAAVAEGKVSTAQIDDNARRMVRLIVRSGVIERGASRPEAATPLRQS-A 331 Query: 302 LIRQLGAEGAVLLKNDGVLPLAKSSFDQIAVIGPNAASARVMGGGSARIAAHYTVSPLEG 361 + R+ E VLLKN+GVLP + S +AVIGPNA R+ GGGS+ + T +PL+ Sbjct: 332 IARKAAEEAMVLLKNNGVLPFDR-SIRSLAVIGPNADVVRMQGGGSSNVVPFETQTPLDA 390 Query: 362 IRAALSNANSLRHAVGCNNNRL-----IDVFSGE-------MTVEYFKGRGFESRPVHVE 409 +RAAL + + G +N +FS + +T YF PV E Sbjct: 391 LRAALPGVR-ITYEKGVDNEETPPAADAKLFSPDGKRGETGLTGSYFMTADASGEPVKTE 449 Query: 410 TVEKGEFFWFDLPSGDLDLADFSARMTATFVPQETGEHIFGMTNAGLARLFVDGELVVDG 469 V + + +G + R F P+ G H F + G R+ +DG+++++ Sbjct: 450 RVTRFVRWISGNVAGPKATGYAAIRWDGMFWPRTGGVHEFSVRGTGTPRVTLDGKVILEK 509 Query: 470 YDGWT-KGENFFGTANSEQRRAVTLGAARRYRVVVEYEAPKASLDGINICALRFGVEKPL 528 + G + VTL A R Y + ++Y + G L FGV +P Sbjct: 510 ASKVVPDNRDVLGFPVPRRTVQVTLEAGRGYPIRIDY-----ANGGTPYENLSFGVREP- 563 Query: 529 GDAGIAEAVETARKSDIVLLLVGREGEWDTEGLDLPDMRLPGRQEELIEAVAETNPNVVV 588 AV A+ +D +++VG + EG D ++ LPG Q L+EA+A NP V Sbjct: 564 -QPSFDAAVAAAKGADAAIVIVGSSSTTEGEGYDRANIDLPGEQNRLVEAIAAANPKTAV 622 Query: 589 VLQTGGPIEMPWLGKVRAVLQMWYPGQELGNALADVLFGDVEPAGRLPQTFPKALTDNSA 648 V+ G + MPW K A++ MW PG+ ALADVL G V P+G+LP TFP+ D+ A Sbjct: 623 VVNAGAAMTMPWHEKAPAIVSMWLPGEGGAAALADVLTGKVNPSGKLPVTFPQRSGDDLA 682 Query: 649 ITDDPSIYPGQDGHVRYAEGIFVGYRHHDTREIEPLFPFGFGLGYTRFTWGAPQLSGTEM 708 + Y+EG+ VGYR + R ++PLFPFG GL YT F + + Sbjct: 683 DL--------KTSKSAYSEGLLVGYRGYQARGVKPLFPFGHGLSYTSFAYSPLNVPARAS 734 Query: 709 GADGLTVTVDVTNIGDRAGSDVVQLYVHSPNARVERPFKELRAFAKLKLAPGATGTAVLK 768 GA +TV + V N G R G +VVQLYV P + LRAF K+ + G L Sbjct: 735 GAKPVTVKLTVRNTGKRTGQEVVQLYVEPVEPMAGDPPRTLRAFQKVAIPAGGKREVTLT 794 Query: 769 IAPRDLAYFDVEAGRFRADAGKYELIVAASAIDIRASVSIHL 810 + PR +++DV+AG +R G Y ++ +S+ DIR + I + Sbjct: 795 LDPRAFSFYDVKAGGWRVRPGAYRVLAGSSSEDIRQAGEIRV 836 Lambda K H 0.318 0.136 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1679 Number of extensions: 80 Number of successful extensions: 7 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 818 Length of database: 838 Length adjustment: 42 Effective length of query: 776 Effective length of database: 796 Effective search space: 617696 Effective search space used: 617696 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 56 (26.2 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory