Alignments for a candidate for etoh-dh-nad in Sphingomonas koreensis DSMZ 15582

GapMind for catabolism of small carbon sources

Alignments for a candidate for etoh-dh-nad in Sphingomonas koreensis DSMZ 15582

Align alcohol dehydrogenase (EC 1.1.1.1) (characterized)
to candidate Ga0059261_2257 Ga0059261_2257 Choline dehydrogenase and related flavoproteins

Query= BRENDA::Q76HN6
         (526 letters)



>FitnessBrowser__Korea:Ga0059261_2257
          Length = 526

 Score =  485 bits (1249), Expect = e-141
 Identities = 260/523 (49%), Positives = 335/523 (64%), Gaps = 6/523 (1%)

Query: 2   EFDYLIVGAGSAGCVLANRLSADPSVTVCLLEAGPEDRSPLIHTPLGLAAILPTRHVNWA 61
           E D +++GAGS G   A RLS D   +V +LEAG   R+    T +  A  + T   NWA
Sbjct: 3   EADIVVIGAGSGGSAAAGRLSEDGKYSVAVLEAG--GRNTGFRTLMPGAIAMQTPATNWA 60

Query: 62  FKTTPQPGLGGRVGYQPRGKVLGGSSSINGMIYIRGHQDDFNDWQALGNEGWGFDDVLPY 121
           ++T PQPGL GR GYQPRGK LGGSS+IN M+YIRG+  D+++W+ALG  GWG+ DVLP 
Sbjct: 61  YETVPQPGLNGRRGYQPRGKGLGGSSAINAMLYIRGNPWDYDNWEALGASGWGWADVLPV 120

Query: 122 FRKSEMHHGGSSEYHGGDGELYVSPANR-HAASEAFVESALRAGHSYNPDFNGATQEGAG 180
           F++SE +  G+S++HGGDG L VS  +  H  S+AFV++A       N DFNG  QEGAG
Sbjct: 121 FKRSETNQRGASQWHGGDGPLQVSDQSFVHPGSQAFVDAAASLQIPRNDDFNGVRQEGAG 180

Query: 181 YYDVTIRDGRRWSTATAFLKPVRHRSNLTVLTHTHVESIVLLGKQATGVQALIKGSRVHL 240
            Y VT +DG RW+ A  +L P   R NL +L  T VE ++    +A GV     G    +
Sbjct: 181 IYQVTQKDGERWTAARGYLTP---RPNLEILCDTVVERVLFEDGRAWGVAYSRGGQSKMI 237

Query: 241 RARKEVILSAGAFGSPHLLMLSGIGSAAELEPQGIAPRHELPGVGQNLQDHADVVLCYKS 300
           RAR+ V+L+ G FG+  LLMLSGIG  A L+  G+  R +   VG NLQDH D V  +++
Sbjct: 238 RARRAVVLAGGVFGTAQLLMLSGIGPGAHLQEHGLTVRIDRGEVGGNLQDHLDYVAAFET 297

Query: 301 NDTSLLGFSLSGGVKMGKAMFDYARHRNGPVASNCAEAGAFLKTDPGLERPDIQLHSVIG 360
             T  LG SL G +K   AM  + R R+GP+ S  AEAGAFL+T P L  PDIQLH ++ 
Sbjct: 298 KGTQFLGRSLIGSLKGLGAMARWFRTRSGPMTSPFAEAGAFLRTRPELPAPDIQLHFLVA 357

Query: 361 TVDDHNRKLHWGHGFSCHVCVLRPKSIGSVGLASPDPRKAPRIDPNFLAHDDDVATLLKG 420
            V+DH R     HG+SCH CVLRP+S G+V LASPDPR AP IDP FL    DV  L+KG
Sbjct: 358 IVEDHGRAKIKTHGYSCHACVLRPESRGTVRLASPDPRAAPLIDPGFLTDRRDVDLLIKG 417

Query: 421 YRITRDIIAQTPMASFGLRDMYSAGLHNDEQLIELLRKRTDTIYHPIGTCKMGQDEMAVV 480
            R    I+   P+ +FG RD Y    ++D  L  L+R R DTIYHP+GT +MG D+ AV 
Sbjct: 418 VRAMYRILDAEPLTAFGGRDRYPLDRNDDAALERLIRARADTIYHPVGTARMGSDDRAVC 477

Query: 481 DSQLRVHGIEGLRVVDASIMPTLVGGNTNAAAIMIAERAAEWI 523
           D +LRV G++GL V DAS+MP L+ GNTNA +IMI ER A++I
Sbjct: 478 DPKLRVRGVDGLYVADASVMPRLISGNTNAPSIMIGERCADFI 520


Lambda     K      H
   0.319    0.137    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 855
Number of extensions: 46
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 526
Length of database: 526
Length adjustment: 35
Effective length of query: 491
Effective length of database: 491
Effective search space:   241081
Effective search space used:   241081
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources