Align Short-chain dehydrogenase (characterized, see rationale)
to candidate Ga0059261_2882 Ga0059261_2882 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)
Query= uniprot:A0A2E7P8M8 (258 letters) >FitnessBrowser__Korea:Ga0059261_2882 Length = 240 Score = 100 bits (248), Expect = 4e-26 Identities = 81/249 (32%), Positives = 117/249 (46%), Gaps = 11/249 (4%) Query: 3 LNLQDKVVIVTGGASGIGGAISLQLAAEGAIPVVFARSEPDPQFWARLTGLQPRAALFQL 62 ++ K +VTGG GIGGAIS L A GA V + + A G A+ L Sbjct: 1 MDFTGKRAVVTGGIGGIGGAISQALHAAGAEVVATGYDQAEVDARAGEAGFAG-IAMKPL 59 Query: 63 ELQDEARCGEAVAETVRRFGRLDGLVNNAGVNDSVGLDAGRNEFVASLERNLIHYYVMAH 122 ++ D+A AV G +D LVN AG F L+ NL Sbjct: 60 DVSDDA----AVRAFAEDTGGIDFLVNCAGTTARGHAAFEEEAFQHVLDVNLTGTMRCCR 115 Query: 123 YCVPHLKATRGAILNVSSKTALTGQGNTSGYCASKGAQLSLTREWAAALRDDGVRVNALI 182 P L GAI+N++S +L G G GY ASKG LT+ A A + +RVNA+ Sbjct: 116 AFRPALAVRGGAIVNIASMMSLFGSGTAPGYAASKGGVALLTKSLAIAWAEQRIRVNAVA 175 Query: 183 PAEVMTPLYEKWIATFENPQEKLDAITSKIPLGKRFTTSEEMADMAVFLLSGRSSHTTGQ 242 P ++TPL ++ I +P + + + + P+ +R+ +AD FL S ++S TG Sbjct: 176 PGWIVTPLTDRQI----DPALR-ERVIGRTPM-RRWGEPRHIADAVAFLCSDKASFITGV 229 Query: 243 WVFVDGGYT 251 + VDGGY+ Sbjct: 230 VLPVDGGYS 238 Lambda K H 0.318 0.134 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 144 Number of extensions: 6 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 258 Length of database: 240 Length adjustment: 24 Effective length of query: 234 Effective length of database: 216 Effective search space: 50544 Effective search space used: 50544 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory