Alignments for a candidate for nagF in Sphingomonas koreensis DSMZ 15582

GapMind for catabolism of small carbon sources

Alignments for a candidate for nagF in Sphingomonas koreensis DSMZ 15582

Align N-Acetyl-D-Glucosamine phosphotransferase system transporter, component of N-acetyl glucosamine-specific PTS permease, GlcNAc IIBC/GlcNAc I-HPr-IIA (characterized)
to candidate Ga0059261_1646 Ga0059261_1646 Phosphocarrier protein HPr/phosphoenolpyruvate--protein phosphotransferase (EC 2.7.3.9)/PTS system IIA component, Glc family (TC 4.A.1)

Query= TCDB::Q9HXN5
         (842 letters)



>FitnessBrowser__Korea:Ga0059261_1646
          Length = 830

 Score =  647 bits (1668), Expect = 0.0
 Identities = 377/831 (45%), Positives = 506/831 (60%), Gaps = 18/831 (2%)

Query: 6   LALKAPLSGPVMPLNRVPDPVFSSGTLGEGIAIDPLNDCLHAPCAGLVSHLARTRHALSL 65
           L + +PL G   PL+ VPDPVF+   +G+G+AI PL D + AP  G V  L    HA+SL
Sbjct: 3   LIITSPLRGWAAPLDSVPDPVFAQRMMGDGVAIQPLADTVCAPFDGEVVTLHEAGHAVSL 62

Query: 66  RADNGAELLLHVGLDTVQLQGEGFEALVEEGARVIEGQPLLRFDLDRVARGSRSLITVMI 125
           R+D GAE+L+H+GLDTV L+G GF  LV  GA V  G PL+RFD+D VA G+ SLIT +I
Sbjct: 63  RSDEGAEVLIHIGLDTVGLKGRGFTPLVAPGATVARGDPLIRFDMDAVALGATSLITPVI 122

Query: 126 LTNGDGFQVRPLTTNPVEVGA--PLLQLSPEKAEQRPANPAPGEGSAQRQVRGRARVAHH 183
           +TN + F +   T     +GA   L+ L+P + + R  +    +G+    +  R  +   
Sbjct: 123 VTNAEAFTITRRTMGRA-IGAHEELMALAPARTQPRRWSD---DGTV---IEERVTLILP 175

Query: 184 GGLHARPAALLRKTAQGFSSQAELHFAGQVASVDSLVGIMGLGVAEQDEVEVICRGEDSE 243
            G+HARPAA L + A+ F ++  L    +     S VG++ LG    DE+ V  RG D+E
Sbjct: 176 HGIHARPAARLGECARQFEAEIHLLHGDKRGDARSTVGLLALGTRLGDELVVQARGSDAE 235

Query: 244 AALGALLAALASATA-GAPKDAPRAIAPGEPARPAAVAGTLAGVCASPGLASGPLARLGA 302
           AAL A++A L S    GAP  AP   A   PA P    G + GV ASPGLA GP ARL  
Sbjct: 236 AALAAIVALLGSDMGEGAPAHAP---AIAVPAAPVLQPGQIGGVIASPGLAMGPAARLRQ 292

Query: 303 ISLPADDGRHRPEEQHLALDQALQRVRDDVQGSLQQARLGGDENEAAIFSAHLALLEDPG 362
            ++  +      + +  ALD A   VR  +      A  GG  + AA+  AHLALL+DP 
Sbjct: 293 TAIAVEREGKGVDAERTALDAARTEVRVRIGARADAA--GG--SVAAVMRAHLALLDDPE 348

Query: 363 LLDAADMLIDQGVGAAHAWHRAIQAQCEILQALGNLLLAERANDLRDLEKRVLRVLLGDT 422
           L   A+  I  G  A +AW  A+  Q   L+A GN  L ER +DL D+E++VL VL G  
Sbjct: 349 LAAGAETRIAAGNSAGYAWRGAVHDQIAALKATGNAHLIERTDDLVDIERQVLGVLTGIP 408

Query: 423 APL-RVPAGAIVAAREITPSDLAPLVDAGAAGLCMAEGGATSHVAILARSKGLPCLVALG 481
           A    + AGAI+ A ++ PS L  +  +  AG+C+  GG TSHVAIL    GLP LVA+G
Sbjct: 409 AEADAIAAGAILVADDLLPSQLVSIAASKPAGICLVRGGPTSHVAILCAGMGLPALVAMG 468

Query: 482 AGLLELEEGRQVVLDAGQGRLELSPDARRLEQVALQVAQREEQRRRQQADAQREALTRDG 541
             +  +E+G  ++LDA  G +   P     +  A ++A+RE +R   +A A     T DG
Sbjct: 469 DAIEAIEDGTALLLDAEAGHVTPDPSQADADAFAARLAKREARRDAARAAAGNACQTADG 528

Query: 542 RRIEIGANVASPREAAEAFANGADGVGLLRTEFLFLERRAAPDEEEQRNAYQEVLDAMGQ 601
            RIE+ AN+ +  +A  A A GA+G GL+R+EFLFL+R  AP E+EQ  AYQ + DA+  
Sbjct: 529 VRIELFANLGTVEDAKAAAAQGAEGSGLVRSEFLFLDREDAPSEDEQHAAYQGIADALPG 588

Query: 602 RKVIIRTIDVGGDKHLDYLPLPVEENPALGLRGIHLGQARPELLDQQLRALLRVEPLERC 661
           + VI+R +DVGGDK   Y+P   EENPALGLRGI +G A  +LL+ Q+RA+LRV+P+ +C
Sbjct: 589 KPVIVRLLDVGGDKPAAYIPFDAEENPALGLRGIRVGLAHRDLLETQIRAILRVQPVGQC 648

Query: 662 RILLPMVSEVDELRAIRRRLGELATQLGIERLPELGVMIEVPSAALLADQLAEHADFLSI 721
           RI+LPMV+ V E+RA+R  +  L  ++GIE   E+GVM+E P+AA+ AD LA  ADFLSI
Sbjct: 649 RIMLPMVASVSEIRAVREVVERLRGEMGIETPIEVGVMVETPAAAITADLLAAEADFLSI 708

Query: 722 GTNDLSQYALAMDRCHAGLADRIDALHPALLRLIAQTCAGAARHGRWVGVCGALASDPLA 781
           GTNDL+QY LAMDR +  +A  +DA+HPA+LR+IA+TC  A   GRWVGVCG LASDP A
Sbjct: 709 GTNDLTQYVLAMDRGNPAVASGVDAMHPAVLRMIAETCRRATAKGRWVGVCGGLASDPAA 768

Query: 782 TPVLVGLGVEELSVGPNLVGEIKTRVRQLDAAECRRHAQALLDLGSARAVR 832
            P+L+GLG  ELS  P  V E K  VR +  A+ R HA+  L   SA  VR
Sbjct: 769 VPILIGLGATELSTVPGFVPEAKAIVRSVTLADARDHAERALHCSSAAEVR 819


Lambda     K      H
   0.319    0.136    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1874
Number of extensions: 95
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 842
Length of database: 830
Length adjustment: 42
Effective length of query: 800
Effective length of database: 788
Effective search space:   630400
Effective search space used:   630400
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources