GapMind for catabolism of small carbon sources

 

Aligments for a candidate for dopDH in Sphingomonas koreensis DSMZ 15582

Align Alpha-ketoglutaric semialdehyde dehydrogenase 1; alphaKGSA dehydrogenase 1; 2,5-dioxovalerate dehydrogenase 1; 2-oxoglutarate semialdehyde dehydrogenase 1; KGSADH-I; Succinate-semialdehyde dehydrogenase [NAD(+)]; SSDH; EC 1.2.1.26; EC 1.2.1.24 (characterized)
to candidate Ga0059261_1680 Ga0059261_1680 NAD-dependent aldehyde dehydrogenases

Query= SwissProt::Q1JUP4
         (481 letters)



>lcl|FitnessBrowser__Korea:Ga0059261_1680 Ga0059261_1680
           NAD-dependent aldehyde dehydrogenases
          Length = 469

 Score =  259 bits (663), Expect = 1e-73
 Identities = 158/473 (33%), Positives = 244/473 (51%), Gaps = 9/473 (1%)

Query: 7   TDTQLLIDGEWVDAASGKTIDVVNPATGKPIGRVAHAGIADLDRALAAAQSGFEAWRKVP 66
           T  +L++DG+    A  +T  V++PATG+P      A  ADLD A+AAA+  F  W   P
Sbjct: 2   TGVRLIVDGK--PLAMAETFPVIDPATGRPFADAPLASTADLDAAVAAARRAFPGWAATP 59

Query: 67  AHERAATMRKAAALVRERADAIAQLMTQEQGKPLTEARVEVLSAADIIEWF-ADEGRRVY 125
             +RAA +   A  +    D +A+L++ EQGKP+  A  E++ A   + W  A  G R  
Sbjct: 60  IEDRAAAILAIADSIEAAKDELARLLSAEQGKPVPNAVGEIMGA---LAWARATAGLRPA 116

Query: 126 GRIVPPRNLGAQQTVVKEPVGPVAAFTPWNFPVNQVVRKLSAALATGCSFLVKAPEETPA 185
             ++   +   +  V ++P+G VA+ +PWNFPV   +  +   L  G + ++K    TP 
Sbjct: 117 VDVLKDDD-SVRVEVHRKPLGVVASISPWNFPVMIAIWHIIPGLVAGNTVVMKPSSFTPL 175

Query: 186 SPAALLRAFVDAGVPAGVIGLVYGDPAEISSYLIPHPVIRKVTFTGSTPVGKQLASLAGL 245
           +   ++    +A +P GV+  V G+  EI   +  HP I K+ FTGSTP G+ + +    
Sbjct: 176 AALRMVE-IANAHLPPGVLNSVTGE-VEIGRAIASHPGIDKIVFTGSTPTGRSIMADGAA 233

Query: 246 HMKRATMELGGHAPVIVAEDADVALAVKAAGGAKFRNAGQVCISPTRFLVHNSIRDEFTR 305
           ++KR T+ELGG+   IV  DADV           F N+GQ+C +  R  VH SI D    
Sbjct: 234 NLKRLTLELGGNDAAIVLPDADVDKVAAKIFAKAFGNSGQICAAVKRVYVHESIHDALAE 293

Query: 306 ALVKHAEGLKVGNGLEEGTTLGALANPRRLTAMASVIDNARKVGASIETGGERIGSEGNF 365
            L + A    VG G +  +  G + N ++   + ++ D+AR  G     GGE    +G F
Sbjct: 294 KLAEMARTAVVGPGSDAASQFGPVQNRKQFDLVRALADDARAHGGRFLAGGEAREGDGYF 353

Query: 366 FAPTVIANVPLDADVFNNEPFGPVAAIRGFDKLEEAIAEANRLPFGLAGYAFTRSFANVH 425
           F  +V+ +V     + + E FGP+  +  +   E+A+A AN    GL G  ++   A   
Sbjct: 354 FPLSVVVDVTDGMRIVDEEQFGPILPVIRYSDPEDALARANANENGLGGSVWSADPAAAL 413

Query: 426 LLTQRLEVGMLWINQPATPWPEMPFGGVKDSGYGSEGGPEALEPYLVTKSVTV 478
              QRLE G +W+N  A+  P++PFGG K SG G+E G   LE Y+  ++V V
Sbjct: 414 AFAQRLEAGTVWVNDHASISPDVPFGGAKQSGVGTEFGLYGLEEYMQLQTVRV 466


Lambda     K      H
   0.318    0.134    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 592
Number of extensions: 30
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 481
Length of database: 469
Length adjustment: 33
Effective length of query: 448
Effective length of database: 436
Effective search space:   195328
Effective search space used:   195328
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory