Align Methionine import ATP-binding protein MetN 2, component of L-Histidine uptake porter, MetIQN (characterized)
to candidate Ga0059261_2293 Ga0059261_2293 cell division ATP-binding protein FtsE
Query= TCDB::Q9HT70 (335 letters) >FitnessBrowser__Korea:Ga0059261_2293 Length = 235 Score = 146 bits (369), Expect = 4e-40 Identities = 82/210 (39%), Positives = 123/210 (58%), Gaps = 5/210 (2%) Query: 1 MIEFHDVHKTYRVAGREIPALQPTRLNIQAGQIFGLIGHSGAGKSTLLRLINRLEEPSGG 60 +++F +V Y G L + AG + + G SGAGK++LLRL+ + P+ G Sbjct: 4 IVQFENVGLRY---GTGAETLSDVSFTLSAGSFYFVTGASGAGKTSLLRLLYLAQRPTRG 60 Query: 61 RILVEGEDVTALDAEGLRRFRQRVGMIFQHFNLLSSKTVADNIAMPLRLAGGFSRAEVDA 120 + + GED AL + L FR+R+G++FQ F LL + DN+A+PLR+A G A+++ Sbjct: 61 IVRLFGEDAGALPRKRLPGFRRRIGVVFQDFRLLPHLSAYDNVALPLRVA-GIPEADIEG 119 Query: 121 RVSELLARVGLSDHARKYPAQLSGGQKQRVGIARALACRPSILLCDEATSALDPQTTASV 180 V E++A VGL D P LSGG++QR+ IARA+ RP IL+ DE T +DP + Sbjct: 120 PVREMIAWVGLKDRDSAKPPTLSGGEQQRIAIARAVITRPEILIADEPTGNVDPDMAERL 179 Query: 181 LQLLAEINRELKLTIVLITHEMDVIRRVCD 210 L L +NR L T+V+ TH+ +I R+ D Sbjct: 180 LHLFDSLNR-LGTTVVVATHDFQLISRIPD 208 Lambda K H 0.322 0.138 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 199 Number of extensions: 11 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 335 Length of database: 235 Length adjustment: 26 Effective length of query: 309 Effective length of database: 209 Effective search space: 64581 Effective search space used: 64581 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory