Align Histidine transport ATP-binding protein HisP (characterized)
to candidate Ga0059261_3653 Ga0059261_3653 phosphate ABC transporter ATP-binding protein, PhoT family (TC 3.A.1.7.1)
Query= SwissProt::P02915 (258 letters) >FitnessBrowser__Korea:Ga0059261_3653 Length = 276 Score = 125 bits (314), Expect = 9e-34 Identities = 87/249 (34%), Positives = 131/249 (52%), Gaps = 22/249 (8%) Query: 16 YGGHEVLKGVSLQARAGDVISIIGSSGSGKSTFLRCINFLEKPS-----EGAIIVNGQNI 70 YG + ++ VS+ +V + IG SG GKSTFLR +N + EG I ++G+NI Sbjct: 38 YGEKQAIRDVSIDVDMENVTAFIGPSGCGKSTFLRTLNRMNDTVASARVEGEITLDGENI 97 Query: 71 NLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFNLWSHMTVLENVMEAP-IQVLGLSKHDA 129 D + V LR R+ MVFQ N + ++ ENV P I L +K D Sbjct: 98 Y-----DKSMDVVQ------LRARVGMVFQKPNPFPK-SIYENVAYGPRIHGLARAKGDM 145 Query: 130 RERALKYLAKVGIDERAQGKYP---VHLSGGQQQRVSIARALAMEPDVLLFDEPTSALDP 186 + + L + G+ E + + LSGGQQQR+ IARA+A++P+V+L DEP SALDP Sbjct: 146 DQIVERSLKRAGLWEEVKDRLNDSGTALSGGQQQRLCIARAIAVDPEVILMDEPCSALDP 205 Query: 187 ELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHVSSHVIFLHQGKIEEEGDPEQVFGNPQS 246 ++ ++ +L +V+VTH M A VS F H G + E G+ +Q+F P+ Sbjct: 206 IATAKIEELIHELRGR-YAIVIVTHNMQQAARVSQRTAFFHLGTLVEYGETDQIFTAPRQ 264 Query: 247 PRLQQFLKG 255 R + ++ G Sbjct: 265 ERTKDYITG 273 Lambda K H 0.319 0.136 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 157 Number of extensions: 10 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 258 Length of database: 276 Length adjustment: 25 Effective length of query: 233 Effective length of database: 251 Effective search space: 58483 Effective search space used: 58483 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory