Align formimidoylglutamate deiminase (EC 3.5.3.13) (characterized)
to candidate Ga0059261_3965 Ga0059261_3965 formimidoylglutamate deiminase (EC 3.5.3.13)
Query= BRENDA::Q9HU77 (453 letters) >FitnessBrowser__Korea:Ga0059261_3965 Length = 447 Score = 434 bits (1116), Expect = e-126 Identities = 229/449 (51%), Positives = 293/449 (65%), Gaps = 5/449 (1%) Query: 4 IFAERALLPEGWARNVRFEISADGVLAEIRPDANADGAERLGGAVLPGMPNLHSHAFQRA 63 ++ E ALL GW+ VR + ADG + + + + + LPG+PN+HSHAFQRA Sbjct: 3 LWFESALLENGWSERVRLTL-ADGQIETVEAGVDPEAGDERHFVALPGIPNVHSHAFQRA 61 Query: 64 MAGLAEVAGNPNDSFWTWRELMYRMVARLSPEQIEVIACQLYIEMLKAGYTAVAEFHYVH 123 MAGLAE G +D FW+WRELMYR V R+ PE+ E IA Y EML++G+T V EFHY+H Sbjct: 62 MAGLAEARGRADDDFWSWRELMYRFVGRIGPEECEAIAALAYAEMLESGFTRVGEFHYLH 121 Query: 124 HDLDGRSYADPAELSLRISRAASAAGIGLTLLPVLYSHAGFGGQPASEGQRRFINGSEAY 183 H G Y D AE+S RI+ AA+A GI LTLLPV Y+H GFGGQPA Q RF+N + + Sbjct: 122 HTPGGGRYDDVAEMSGRIAAAAAATGIALTLLPVFYAHGGFGGQPAGTAQARFLNDVDGF 181 Query: 184 LELLQRLRAPLEAAGHSLGLCFHSLRAVTPQQIATVLAAGHDDLPVHIHIAEQQKEVDDC 243 L++R A L + G +G+ HSLRA TP ++ +LA D PVHIHIAEQ KEV DC Sbjct: 182 AALVERAGATLASDG-VIGIAPHSLRAATPGELRALLAMA-DRGPVHIHIAEQVKEVADC 239 Query: 244 QAWSGRRPLQWLYENVAVDQRWCLVHATHADPAEVAAMARSGAVAGLCLSTEANLGDGIF 303 AWSG+RP++WL +N+ VD RW LVHATH +P EVA +A SGAVAGLC TEANLGDG+F Sbjct: 240 VAWSGKRPVRWLLDNMPVDARWTLVHATHVEPGEVAGIAASGAVAGLCPITEANLGDGVF 299 Query: 304 PATDFLAQGGRLGIGSDSHVSLSVVEELRWLEYGQRLRDRKRNRLYRDDQPMIGRTLYDA 363 PA +F+A GG +GIGSDS+V + EELR LEYGQRL R RN L D+P G ++ A Sbjct: 300 PAAEFMALGGMIGIGSDSNVRIDAAEELRLLEYGQRLTRRARNVLAGGDRPATGARMFAA 359 Query: 364 ALAGGAQALGQPIGSLAVGRRADLLVLDGNDPYLASAEGDALLNRWLFAGGDRQVRDVMV 423 A+AGG ++LG G LA GR AD++ L+ +DP A +GDAL++ W+FA V V Sbjct: 360 AVAGGGRSLGVETG-LAAGRPADIVSLNRDDPAFAERKGDALVDSWVFA-SRAGVDCVWR 417 Query: 424 AGRWVVRDGRHAGEERSARAFVQVLGELL 452 G V GRH + + L L+ Sbjct: 418 GGAKQVAGGRHRDRDAIEARYRVALNRLM 446 Lambda K H 0.321 0.136 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 637 Number of extensions: 30 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 453 Length of database: 447 Length adjustment: 33 Effective length of query: 420 Effective length of database: 414 Effective search space: 173880 Effective search space used: 173880 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 51 (24.3 bits)
Align candidate Ga0059261_3965 Ga0059261_3965 (formimidoylglutamate deiminase (EC 3.5.3.13))
to HMM TIGR02022 (hutF: formiminoglutamate deiminase (EC 3.5.3.13))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR02022.hmm # target sequence database: /tmp/gapView.30503.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR02022 [M=455] Accession: TIGR02022 Description: hutF: formiminoglutamate deiminase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 5.1e-189 614.6 4.1 5.7e-189 614.5 4.1 1.0 1 lcl|FitnessBrowser__Korea:Ga0059261_3965 Ga0059261_3965 formimidoylglutam Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Korea:Ga0059261_3965 Ga0059261_3965 formimidoylglutamate deiminase (EC 3.5.3.13) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 614.5 4.1 5.7e-189 5.7e-189 4 454 .. 3 446 .. 1 447 [] 0.98 Alignments for each domain: == domain 1 score: 614.5 bits; conditional E-value: 5.7e-189 TIGR02022 4 yfaerallpdgwaegvrlavaedGrilavetgvsaaedaerlsgvvlpglanlHsHAFqralaGlaeva 72 ++ e+all +gw+e vrl++a dG+i +ve+gv ++er v+lpg++n+HsHAFqra+aGlae + lcl|FitnessBrowser__Korea:Ga0059261_3965 3 LWFESALLENGWSERVRLTLA-DGQIETVEAGVDPEAGDERHF-VALPGIPNVHSHAFQRAMAGLAEAR 69 799*****************9.88*********9877777777.9************************ PP TIGR02022 73 gsgaDsFWtWRevmYrlverldPeqleaiarqlyvemlkaGftrvgEFHYlHHaadGtpYadpaelaer 141 g+++D+FW+WRe+mYr+v r+ Pe+ eaia+ +y+eml++GftrvgEFHYlHH ++G +Y+d ae++ r lcl|FitnessBrowser__Korea:Ga0059261_3965 70 GRADDDFWSWRELMYRFVGRIGPEECEAIAALAYAEMLESGFTRVGEFHYLHHTPGGGRYDDVAEMSGR 138 ********************************************************************* PP TIGR02022 142 iaaAAadaGigltlLpvlYahagFGgaaanegqrrfiddveaflrlvealrkelaaqeearlGlaiHsl 210 iaaAAa +Gi ltlLpv+Yah gFGg++a ++q rf++dv+ f++lve+ +la ++ ++G+a+Hsl lcl|FitnessBrowser__Korea:Ga0059261_3965 139 IAAAAAATGIALTLLPVFYAHGGFGGQPAGTAQARFLNDVDGFAALVERAGATLA--SDGVIGIAPHSL 205 ***************************************************9998..7889******** PP TIGR02022 211 RAvtaeelaavlqaserqlPvHiHvaEqqkEvddClaasgrrPvewLldhaevdarwclvHatHltdee 279 RA+t+ el+a+l++++r PvHiH+aEq kEv dC+a+sg+rPv+wLld+++vdarw+lvHatH+++ e lcl|FitnessBrowser__Korea:Ga0059261_3965 206 RAATPGELRALLAMADRG-PVHIHIAEQVKEVADCVAWSGKRPVRWLLDNMPVDARWTLVHATHVEPGE 273 **************9996.************************************************** PP TIGR02022 280 vkllaksgavaglCpttEanLgDGifpaadfvaaggrlgiGsDshvvvdvleElRllEygqRLrdraRn 348 v+ +a sgavaglCp+tEanLgDG+fpaa+f+a gg +giGsDs+v +d++eElRllEygqRL++raRn lcl|FitnessBrowser__Korea:Ga0059261_3965 274 VAGIAASGAVAGLCPITEANLGDGVFPAAEFMALGGMIGIGSDSNVRIDAAEELRLLEYGQRLTRRARN 342 ********************************************************************* PP TIGR02022 349 vlaageeasvaralydaAlaggaqalGlaegeleaGarADlltldledpslagakgdalldsllfaaek 417 vla g++++++++++ aA+agg ++lG ++ +l+aG +AD+++l+ +dp+ a++kgdal+ds++fa+++ lcl|FitnessBrowser__Korea:Ga0059261_3965 343 VLAGGDRPATGARMFAAAVAGGGRSLGVET-GLAAGRPADIVSLNRDDPAFAERKGDALVDSWVFASRA 410 ***************************975.7***********************************76 PP TIGR02022 418 aavrdvvvaGrkvvrdgrHaleeeierafakvlrall 454 v+ v+++G k+v +grH+++++ie ++ +l++l+ lcl|FitnessBrowser__Korea:Ga0059261_3965 411 -GVDCVWRGGAKQVAGGRHRDRDAIEARYRVALNRLM 446 .9*******************************9987 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (455 nodes) Target sequences: 1 (447 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.02 # Mc/sec: 7.25 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory