Align acetyl-CoA acetyltransferase (EC 2.3.1.16) (characterized)
to candidate Ga0059261_0982 Ga0059261_0982 acetyl-CoA acetyltransferases
Query= metacyc::MONOMER-13585 (391 letters) >FitnessBrowser__Korea:Ga0059261_0982 Length = 390 Score = 556 bits (1432), Expect = e-163 Identities = 283/390 (72%), Positives = 326/390 (83%) Query: 1 MTNVVIVSAARTAVGSFNGAFASTPAHDLGAAVIEAVVARAGIDKADVSETILGQVLTAG 60 MT VVI +A RT VGSF GAFASTPAH+LG IEA + +A + +VSE ILGQVLTA Sbjct: 1 MTEVVITAAKRTPVGSFLGAFASTPAHELGRIAIEAALEQAEVKGEEVSEVILGQVLTAA 60 Query: 61 QGQNPARQAHIKAGLPQESAAWSINQVCGSGLRAVALAAQHVQLGDASIVVAGGQENMSL 120 QGQNPARQA + AG+P+E AW +NQVCGSGLRAVALAAQ VQ GDA+IVVAGGQE+MSL Sbjct: 61 QGQNPARQASMAAGVPKEVPAWGLNQVCGSGLRAVALAAQAVQTGDATIVVAGGQESMSL 120 Query: 121 SPHVAHLRAGQKMGDLSFIDSMIKDGLWDAFNGYHMGQTAENVAAKWQISRDMQDEFAVA 180 + H LRAG KMGD+ ID+MIKDGL D FNGYHMG TAEN+A ++Q++R QDEFAV Sbjct: 121 AAHAQSLRAGAKMGDVGLIDTMIKDGLTDVFNGYHMGITAENLAEQYQVTRGEQDEFAVR 180 Query: 181 SQNKAEAAQKAGRFADEIVPFVIKTRKGDVTVDADEYIRHGATLDAMAKLRPAFIKDGTV 240 SQN AEAA+ GRF DEI P IK RKGD VD DEYIR GATLD++A LRPAF KDGTV Sbjct: 181 SQNLAEAARSEGRFKDEIAPVTIKGRKGDTVVDQDEYIRAGATLDSVAGLRPAFKKDGTV 240 Query: 241 TAANASGINDGAAAVLVMSAEEAEKRGLSPLARIASYATAGLDPSIMGVGPIHASRKALE 300 TA NASG+NDGAAA++VMS EEA+KRG + LA I S+ATAG+DPSIMG+GP+ AS+KALE Sbjct: 241 TAGNASGLNDGAAALVVMSREEADKRGATVLATIKSWATAGVDPSIMGIGPVPASKKALE 300 Query: 301 KAGWKVGDLDLVEANEAFAAQACAVNKDMGWDPSIVNVNGGAIAIGHPIGASGARVLNTL 360 KAGW V DLDL+EANEAFAAQA +V K++G+DP+ VNVNGGAIAIGHPIGASGARVL TL Sbjct: 301 KAGWTVADLDLIEANEAFAAQALSVGKELGFDPNKVNVNGGAIAIGHPIGASGARVLTTL 360 Query: 361 LFEMQRRNAKKGLATLCIGGGMGVAMCLER 390 L+EMQRR+AKKGLATLCIGGGMG+AMC+ER Sbjct: 361 LYEMQRRDAKKGLATLCIGGGMGIAMCVER 390 Lambda K H 0.317 0.130 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 477 Number of extensions: 12 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 391 Length of database: 390 Length adjustment: 31 Effective length of query: 360 Effective length of database: 359 Effective search space: 129240 Effective search space used: 129240 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 50 (23.9 bits)
Align candidate Ga0059261_0982 Ga0059261_0982 (acetyl-CoA acetyltransferases)
to HMM TIGR01930 (acetyl-CoA C-acyltransferase (EC 2.3.1.16))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR01930.hmm # target sequence database: /tmp/gapView.28448.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01930 [M=385] Accession: TIGR01930 Description: AcCoA-C-Actrans: acetyl-CoA C-acyltransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 5.2e-146 472.4 11.9 6e-146 472.2 11.9 1.0 1 lcl|FitnessBrowser__Korea:Ga0059261_0982 Ga0059261_0982 acetyl-CoA acetyl Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Korea:Ga0059261_0982 Ga0059261_0982 acetyl-CoA acetyltransferases # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 472.2 11.9 6e-146 6e-146 1 385 [] 6 389 .. 6 389 .. 0.98 Alignments for each domain: == domain 1 score: 472.2 bits; conditional E-value: 6e-146 TIGR01930 1 ivdavRtpigklggslkelsaedLlaavikelleragldpekidevilGnvlqageqaniaReaalaag 69 i +a Rtp+g++ g++++++a++L++ +i+++le+a+++ e++ evilG+vl+a++++n+aR+a +aag lcl|FitnessBrowser__Korea:Ga0059261_0982 6 ITAAKRTPVGSFLGAFASTPAHELGRIAIEAALEQAEVKGEEVSEVILGQVLTAAQGQNPARQASMAAG 74 7799***************************************************************** PP TIGR01930 70 lpesvpaltvnrvCaSglqAvalaaqkikaGeadvvvaGGvEsmSrvpillkaslrreslklgkakled 138 +p++vpa+ +n+vC+Sgl+Avalaaq++++G+a++vvaGG EsmS + + + r+++k+g+ l d lcl|FitnessBrowser__Korea:Ga0059261_0982 75 VPKEVPAWGLNQVCGSGLRAVALAAQAVQTGDATIVVAGGQESMSLAAHAQSL---RAGAKMGDVGLID 140 ***********************************************865544...799********** PP TIGR01930 139 qllkdl...vktklsmgetAenlakkygisReeqDeyalrShqkaakAieegkfkdeivpvevkgk..k 202 +++kd+ v ++++mg+tAenla++y+++R eqDe+a+rS++ a++A +eg+fkdei pv++kg+ + lcl|FitnessBrowser__Korea:Ga0059261_0982 141 TMIKDGltdVFNGYHMGITAENLAEQYQVTRGEQDEFAVRSQNLAEAARSEGRFKDEIAPVTIKGRkgD 209 *********99********************************************************99 PP TIGR01930 203 kvvskDegirpnttlekLakLkpafkekkgstvtAgNssqlnDGAaalllmseevakelgltplarivs 271 +vv++De ir++ tl+++a+L+pafk+ +g tvtAgN+s+lnDGAaal++ms+e a++ g t+la+i s lcl|FitnessBrowser__Korea:Ga0059261_0982 210 TVVDQDEYIRAGATLDSVAGLRPAFKK-DG-TVTAGNASGLNDGAAALVVMSREEADKRGATVLATIKS 276 9************************95.9*.6************************************* PP TIGR01930 272 aavagvdpeemglgpvpAiekaLkkaglsisdidlvEinEAFAaqvlavekelgsldlekvNvnGGAiA 340 +a+agvdp++mg+gpvpA++kaL+kag+++ d+dl+E nEAFAaq+l v kelg d++kvNvnGGAiA lcl|FitnessBrowser__Korea:Ga0059261_0982 277 WATAGVDPSIMGIGPVPASKKALEKAGWTVADLDLIEANEAFAAQALSVGKELG-FDPNKVNVNGGAIA 344 ******************************************************.78************ PP TIGR01930 341 lGHPlGasGarivltllkeLkergkkyGlatlCvggGqGaAvile 385 +GHP+GasGar+++tll+e+++r++k GlatlC+ggG+G+A+ +e lcl|FitnessBrowser__Korea:Ga0059261_0982 345 IGHPIGASGARVLTTLLYEMQRRDAKKGLATLCIGGGMGIAMCVE 389 ******************************************987 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (385 nodes) Target sequences: 1 (390 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.02 # Mc/sec: 7.16 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory