Align beta-galactosidase (EC 3.2.1.23) (characterized)
to candidate Ga0059261_2643 Ga0059261_2643 Beta-galactosidase
Query= BRENDA::B9LW38 (700 letters) >FitnessBrowser__Korea:Ga0059261_2643 Length = 687 Score = 370 bits (951), Expect = e-107 Identities = 225/620 (36%), Positives = 312/620 (50%), Gaps = 45/620 (7%) Query: 3 LGVCYFPEHWPSEEWERDVAAMADAGLEYVRMAEFSWGVLEPERGTFDFEWLDEAIELIG 62 +GV ++PE WP + W D+ M +G VR+ EF+W +EPE G FDF W+D AI Sbjct: 39 VGVAWYPEQWPEDRWAVDLDMMKASGFNTVRIGEFAWARMEPEEGKFDFAWMDRAIAAAV 98 Query: 63 DHGMQAVLCTPTATPPKWLVDERPSIRQEDPDGTVREHGSRRHYCFNSDAYREETARIVE 122 G V+ TP+A PP WL P + D +G HG RRH+ F S YRE + RI Sbjct: 99 KRGFMVVIGTPSAAPPAWLTQNYPDTLRVDENGQRASHGGRRHFSFASRRYRELSRRIAV 158 Query: 123 RVTERYADSPHVAGWQTDNEFGCHETVRCYCDDCADAFRTWLADRYGDIDRLNEAWGNAF 182 + RY P V GWQ DNE G + + A+ +L RYG ID LN W + Sbjct: 159 EMATRYGKHPSVVGWQVDNEVG----PPSWDAESVAAWHAFLKQRYGTIDALNRRWTTQY 214 Query: 183 WSQQYGSFDEIDPPGPTPAEHHPSRLLAYARFSSDSVVEYNRLHADLIRE-ADPDWFVTH 241 WSQ Y + +I P + +P LL + F++ +Y + A IR D ++T Sbjct: 215 WSQFYNDWSQI--PLRQTGQQNPGLLLDFRHFTTAVWTDYIQNQARAIRPLIDRRAWITT 272 Query: 242 NFMGRFPTLNAYDVSEDLDRVAWDSYPTGFVQDRYDGEASPDQLRAGDPDQV--GMDHDI 299 N M + + + DLD +WD+Y PD G PD V G +H + Sbjct: 273 NTMFWNAGFDHFVMHRDLDLASWDNY-------------IPD----GRPDWVANGANHAL 315 Query: 300 YRSALDRPFWVMEQQPGDVNWPPHCPQPGEGAMRLWAHHAAAHGADAVLYFRWRRCLEGQ 359 R R FW+ME QPG V+W P G +R A + +HG+D VLY++WR GQ Sbjct: 316 VRGYKQRNFWLMETQPGRVDWVPVNRALDPGQVRELAWQSVSHGSDGVLYWQWRPARNGQ 375 Query: 360 EQYHAGLRKADGSPDRGYADAAHTSEEF---ATLDGASHVDAPVAVVFDYDSLWALNAQP 416 E YH + DG P+ +A+ ++E A L + A VA+++ YDS WA++ Q Sbjct: 376 ETYHGAVLGQDGEPNPIHAEITQIAKELTAAAPLLADTGPAAKVAMLWSYDSRWAIDLQR 435 Query: 417 HAPDFDYWALQEAFYGAVRGRGVQVDVVPPSADLSGYAAVVAPALHLVTEDLADRLTDYI 476 H DFD Y +R + V VV P A L+ Y VVAP L++VT+ ADRL Y+ Sbjct: 436 HHRDFDPVKAFTDVYRPLRVQSQGVHVVSPDAALAAYPLVVAPNLNVVTQAQADRLAAYV 495 Query: 477 AGGGEVLFGPRTGVKDAENKLRPMSQPGPLTDLVGATVDQHESLPRRLETTVRRVGDPTD 536 GGG ++ GPR+G+KD N L P QPGPL+DL+GA V+Q+ +L P Sbjct: 496 RGGGHLVLGPRSGMKDDANALWPQRQPGPLSDLLGAMVEQYYALDA-----------PVG 544 Query: 537 DSEEIAAPPVSFRTWAEWLDPDAAEPQYA---YDVDGPADGRPAVVTNTVGDGQVTYCGV 593 E+ V+ WAE + PDA + + D G DG+PA VT VG G +TY G Sbjct: 545 IKGELGKGKVAI--WAEEIRPDAVDVRVIATYTDPGGWLDGKPAAVTRKVGRGSITYFGA 602 Query: 594 WPESDLADALASDLLDRAGV 613 W + + LA+ LL A + Sbjct: 603 WLDPATMNTLAARLLGEAKI 622 Lambda K H 0.318 0.137 0.444 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1658 Number of extensions: 98 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 700 Length of database: 687 Length adjustment: 39 Effective length of query: 661 Effective length of database: 648 Effective search space: 428328 Effective search space used: 428328 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory