GapMind for catabolism of small carbon sources

 

Aligments for a candidate for amaB in Sphingomonas koreensis DSMZ 15582

Align Putative aldehyde dehydrogenase transmembrane protein; EC 1.2.1.3 (characterized, see rationale)
to candidate Ga0059261_1680 Ga0059261_1680 NAD-dependent aldehyde dehydrogenases

Query= uniprot:Q92L07
         (510 letters)



>lcl|FitnessBrowser__Korea:Ga0059261_1680 Ga0059261_1680
           NAD-dependent aldehyde dehydrogenases
          Length = 469

 Score =  211 bits (538), Expect = 4e-59
 Identities = 151/468 (32%), Positives = 217/468 (46%), Gaps = 17/468 (3%)

Query: 25  KDLYTGGDMPSFSPVTGEKIASLKTVSAAEAAGKIEKADEAFRAWRLVPAPKRGELVRLL 84
           K L      P   P TG   A     S A+    +  A  AF  W   P   R   +  +
Sbjct: 11  KPLAMAETFPVIDPATGRPFADAPLASTADLDAAVAAARRAFPGWAATPIEDRAAAILAI 70

Query: 85  GEELRAFKADLGRLVSIEAGKIPSEGLGEVQEMIDICDFAVGLSRQLYGLTIATERPGHR 144
            + + A K +L RL+S E GK     +GE+   +       GL      + +  +    R
Sbjct: 71  ADSIEAAKDELARLLSAEQGKPVPNAVGEIMGALAWARATAGLRP---AVDVLKDDDSVR 127

Query: 145 MMETWHPLGVVGIISAFNFPVAVWSWNAALALVCGDAVVWKPSEKTPLTALACQAILERA 204
           +     PLGVV  IS +NFPV +  W+    LV G+ VV KPS  TPL AL    I    
Sbjct: 128 VEVHRKPLGVVASISPWNFPVMIAIWHIIPGLVAGNTVVMKPSSFTPLAALRMVEIANAH 187

Query: 205 IARFGDAPEGLSQVLIGDRAIGEVLVDHPKVPLVSATGSTRMGREVGPRLAKRFARAILE 264
           +      P G+   + G+  IG  +  HP +  +  TGST  GR +    A    R  LE
Sbjct: 188 L------PPGVLNSVTGEVEIGRAIASHPGIDKIVFTGSTPTGRSIMADGAANLKRLTLE 241

Query: 265 LGGNNAGIVCPSADLDMALRAIAFGAMGTAGQRCTTLRRLFVHESVYDQLVPRLKKAYQS 324
           LGGN+A IV P AD+D     I   A G +GQ C  ++R++VHES++D L  +L +  ++
Sbjct: 242 LGGNDAAIVLPDADVDKVAAKIFAKAFGNSGQICAAVKRVYVHESIHDALAEKLAEMART 301

Query: 325 VSVGNPLESAALVGPLVDKAAFDGMQKAIAEAKNHGGAVTGGERVELGHENGYYVKPALV 384
             VG   ++A+  GP+ ++  FD ++    +A+ HGG    G     G  +GY+  P  V
Sbjct: 302 AVVGPGSDAASQFGPVQNRKQFDLVRALADDARAHGGRFLAGGEAREG--DGYFF-PLSV 358

Query: 385 EMPKQEGP--VLEETFAPILYVMKYSDFDAVLAEHNAVAAGLSSSIFTRDMQESERFLAA 442
            +   +G   V EE F PIL V++YSD +  LA  NA   GL  S+++ D   +  F  A
Sbjct: 359 VVDVTDGMRIVDEEQFGPILPVIRYSDPEDALARANANENGLGGSVWSADPAAALAF--A 416

Query: 443 DGSDCGIANVNIGTSGAEIGGAFGGEKETGGGRESGSDAWKAYMRRAT 490
              + G   VN   S       FGG K++G G E G    + YM+  T
Sbjct: 417 QRLEAGTVWVNDHAS-ISPDVPFGGAKQSGVGTEFGLYGLEEYMQLQT 463


Lambda     K      H
   0.317    0.134    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 577
Number of extensions: 25
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 510
Length of database: 469
Length adjustment: 34
Effective length of query: 476
Effective length of database: 435
Effective search space:   207060
Effective search space used:   207060
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory