Alignments for a candidate for susB in Sphingomonas koreensis DSMZ 15582

GapMind for catabolism of small carbon sources

Alignments for a candidate for susB in Sphingomonas koreensis DSMZ 15582

Align Probable alpha-glucosidase; EC 3.2.1.20 (uncharacterized)
to candidate Ga0059261_1580 Ga0059261_1580 Glycosidases

Query= curated2:Q9Z3R8
         (551 letters)



>FitnessBrowser__Korea:Ga0059261_1580
          Length = 541

 Score =  534 bits (1375), Expect = e-156
 Identities = 261/497 (52%), Positives = 328/497 (65%), Gaps = 8/497 (1%)

Query: 12  EPDRDWWRGAVIYQIYPRSFQDTNGDGIGDLQGITARLPHIAGLGADAIWISPFFTSPMR 71
           E +RDWWRGAVIYQIYPRSF D+NGDGIGDL G+ A L H+A LGADAIW+SPFFTSPMR
Sbjct: 11  ETNRDWWRGAVIYQIYPRSFADSNGDGIGDLPGVAAHLDHVAALGADAIWLSPFFTSPMR 70

Query: 72  DFGYDVSNYVDVDPIFGTLEDFDALIAEAHRLGLRVMIDLVLSHTSDRHPWFVESRSSRS 131
           DFGYD+++Y  VDP+FGTL DFDA+I +AH LGL+V+ID V SHTSD+H WF ESRSSR 
Sbjct: 71  DFGYDIADYCGVDPVFGTLADFDAVIEKAHALGLKVIIDQVYSHTSDQHEWFKESRSSRD 130

Query: 132 NAKADWYVWADSKPDGTPPNNWLSIFGGSAWQWDPTRLQYYLHNFLTSQPDLNLHNPQVQ 191
           NAKADWYVWAD+K DG+PP NW S+FGG AW WD  R QYYLHNFL+ QPDLNLHNPQVQ
Sbjct: 131 NAKADWYVWADAKADGSPPTNWQSVFGGPAWTWDARRGQYYLHNFLSEQPDLNLHNPQVQ 190

Query: 192 EALLAVERFWLERGVDGFRLDTINFYFHDRELRDNPALVPERRNASTAPAVNPYNYQEHI 251
            A LA  RFWL+RGVDGFR+D INF  HD E RDNP         +  P   P+++Q+H+
Sbjct: 191 NAALAAARFWLDRGVDGFRVDAINFAMHDPEFRDNPP-----APYTGTPRTRPFDFQQHL 245

Query: 252 YDKNRPENLEFLKRFRAVMDEFPAIAAVGEVGDSQRGLEIAGEYTSGGDKVHMCYAFEFL 311
           Y+++ P+ + FL+R R + D +     V EVG      E+   +T G  +++  Y F+FL
Sbjct: 246 YNQSHPDIVRFLERHRELTDSYGGRFTVAEVGGPTPEAEMHA-FTQGQKRLNSAYGFDFL 304

Query: 312 APDRLTPQRVAEVLRDFHRAAPEGWACWAFSNHDVVRHVSRWADGVTDHDAHAKLLASLL 371
             ++LTP  V + +  +      GW  WAF NHD  R +SRW D     DA A +   LL
Sbjct: 305 YANKLTPALVKKAVETWPDTHGTGWPSWAFENHDAPRALSRWVD-AEHRDAFAAMKMLLL 363

Query: 372 MSLRGTVCIYQGEELALAEAELDYEDLQDPYGIQFWPDFKGRDGCRTPMVWESLPDG-GF 430
           M LRG   ++QGEEL L + E+ Y+ LQDP  I  WP    RDG RTPM W++  +  GF
Sbjct: 364 MCLRGNAILFQGEELGLTQVEIAYDQLQDPEAIANWPLTLSRDGVRTPMPWKADAENLGF 423

Query: 431 SSATPWLPISQSHIPRAVAVQEGDPASVLHHYRRFLAFRKANPALAKGEIEFVETRGSLL 490
           ++  PWLP+   H   AV  Q     S+L+  R  +A R ANPAL  G +E +    +LL
Sbjct: 424 TAGRPWLPLGPDHGALAVDRQIAQNGSLLNLTRGLIALRAANPALRAGAMEVLAADEALL 483

Query: 491 GFLRSHGNEKVFCLFNM 507
            F R    + + C FN+
Sbjct: 484 VFTRIADGQALTCAFNL 500


Lambda     K      H
   0.321    0.138    0.443 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1018
Number of extensions: 54
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 551
Length of database: 541
Length adjustment: 35
Effective length of query: 516
Effective length of database: 506
Effective search space:   261096
Effective search space used:   261096
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources