Alignments for a candidate for N515DRAFT_2924 in Sphingomonas koreensis DSMZ 15582

GapMind for catabolism of small carbon sources

Alignments for a candidate for N515DRAFT_2924 in Sphingomonas koreensis DSMZ 15582

Align Basic amino acid/polyamine antiporter, APA family (characterized, see rationale)
to candidate Ga0059261_1577 Ga0059261_1577 amino acid/polyamine/organocation transporter, APC superfamily (TC 2.A.3)

Query= uniprot:A0A1I1Y8J0
         (492 letters)



>FitnessBrowser__Korea:Ga0059261_1577
          Length = 470

 Score =  347 bits (890), Expect = e-100
 Identities = 200/488 (40%), Positives = 278/488 (56%), Gaps = 37/488 (7%)

Query: 1   MLKNLFATTQISPASADLPGGGAHGEATLKRALTARHLVLLGIGAIIGAGIFVITGQAAA 60
           M   LF T ++  A+   P      E  L   L+  HLV LG+GAI+G GI  + G  A 
Sbjct: 1   MAGGLFRTKRVKDAAEQAP------EHRLAATLSWPHLVALGVGAIVGTGILTLIGVGAG 54

Query: 61  EHAGPAIVLSFVFAGIACALAALCYAEFAAMLPVSGSAYSYSYATLGEYVAWFVGWSLVL 120
           + AGPA+++SFV AG  CA AAL YAE A M+P SGSAY+YSYA LGE +AW VGWSL+L
Sbjct: 55  K-AGPAVIMSFVIAGAICACAALAYAEMATMMPASGSAYAYSYAVLGEIIAWVVGWSLIL 113

Query: 121 EYLFTVATVAAGWSGYFNKLLALISGWIGHDVSLPQTLAAAPFTVVDGHIQATGMFINLP 180
           EY   V+TVA GWSGY      L+  W G    +P  L A P         A G+ +NLP
Sbjct: 114 EYSLVVSTVAVGWSGY---AAPLLHAWTG----MPLELMAGP--------HANGI-VNLP 157

Query: 181 AVAIIAAITGLCYVGITQSAFVNSIIVAIKVTVILLFIAFATKYINPDNWHPFIP----- 235
           A+ IIA + GL  +G  +SA +N+ +V +K+  + +F+A A  Y N  N  PF P     
Sbjct: 158 AIFIIAVVAGLLCLGTKESATLNAALVVVKIIALAVFVAVALPYFNGANLEPFAPFGFAK 217

Query: 236 --ASEGASKYGWAGVGRAAAIVFFSYIGFDAVSTAAGEAKNPQRDMPIGIIGSLILCTIL 293
             + +G  +    GV  AAAI+FF++ GFDA+STAA E KNP RD+ IGI+GS+I C  +
Sbjct: 218 TISPDGVER----GVMAAAAIIFFAFYGFDAISTAAEETKNPGRDLAIGIVGSMIACVAI 273

Query: 294 YIIVAGILTGIADF-RLLGTPEPVSTALDNYPSLHWLQIIVVIGAVTGLSSVMLVMLMGQ 352
           Y++VA    G   F     +PEP++  L +     +   + V  A+  L +V+L  L GQ
Sbjct: 274 YMLVAVAAVGATPFTHFANSPEPLALILRDLGRPGFATFLAV-SAIIALPTVLLGFLFGQ 332

Query: 353 PRIFYSMARDGLIPAVFGRIHQKFRTPHVGTVVVGVLAAALGGLFNIGVLGEMVAMGTLL 412
            RIF++MARDG++P    ++  K  +P   T+    + A + GL  I  +  +   GTL 
Sbjct: 333 SRIFFTMARDGMLPIGLAKV-SKRGSPVRITLFTAAIVAVIAGLLPIDEIAALANAGTLA 391

Query: 413 AFATVCIGVLVLRYTRPELPRAFRVPVPWIVCPLGALACMALFLQSFLEHWRWMLAWIAI 472
           AF  V + ++VLR   P++PR FR P+ W+V  +  L C+ LF    ++   W LAW A+
Sbjct: 392 AFTAVAVCMMVLRVRAPDMPRMFRTPLWWLVGAIAVLGCIYLFFSLPVKTQLWFLAWNAL 451

Query: 473 GQAIYFLY 480
           G  IYF Y
Sbjct: 452 GVVIYFAY 459


Lambda     K      H
   0.328    0.141    0.441 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 730
Number of extensions: 44
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 492
Length of database: 470
Length adjustment: 34
Effective length of query: 458
Effective length of database: 436
Effective search space:   199688
Effective search space used:   199688
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources