GapMind for catabolism of small carbon sources

 

Alignments for a candidate for LRA1 in Sphingomonas koreensis DSMZ 15582

Align NAD(P)+-dependent L-rhamnose 1-dehydrogenase (EC 1.1.1.378; EC 1.1.1.173) (characterized)
to candidate Ga0059261_0844 Ga0059261_0844 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Query= metacyc::MONOMER-16230
         (256 letters)



>FitnessBrowser__Korea:Ga0059261_0844
          Length = 254

 Score =  153 bits (387), Expect = 3e-42
 Identities = 95/252 (37%), Positives = 141/252 (55%), Gaps = 4/252 (1%)

Query: 3   LIDKTVIVTGASRGIGRAAARECARQGARVVIGHSGSDEGRAGALSLAEEIAAFGGTAIA 62
           L  K  IVTG+SRGIG+A+A   A  GA+VVI     D     A ++  +  A  GTAIA
Sbjct: 6   LTGKVAIVTGSSRGIGKASAEALADHGAKVVISSRKQDACDEVAAAINAKHGA--GTAIA 63

Query: 63  VGADAADLDSGEKLVAAAVEAFGSVDVLVNNAGICPFHSFL-DMPRELYLKTVGTNLNGA 121
           V A  +D  + + LV     AFG +D+LV NA   P++  L  +  + + K +  N+   
Sbjct: 64  VAASISDKAALQNLVDETRRAFGRIDILVCNAASNPYYGPLAGIEDDQFRKILDNNIISN 123

Query: 122 YFTVQAAARRMKEQGRGGAIIAVSSISALVGGAMQTHYTPTKAGLLSLMQSCAIALGPYG 181
           ++ +Q  A  M+E+ + GAI+ VSSI  L G  +   Y  +KA    L ++ A+  GP  
Sbjct: 124 HWLIQMTAPEMRER-KDGAIVIVSSIGGLRGSPVIGAYNVSKAADFQLARNYAVEYGPDN 182

Query: 182 IRCNAVLPGTIATDINKEDLSDLEKRERMTSRVPLGRLGEPDDLAGPIVFLASDMARYVT 241
           +R N + PG I TD  +    D    +R+    PL RLGEP+D+AG +V+LAS   RY+T
Sbjct: 183 VRVNCIAPGLIRTDFARALWEDPAAEKRVNQGTPLRRLGEPEDIAGAVVYLASPAGRYMT 242

Query: 242 GASLLVDGGLFV 253
           G +++VDGG+ +
Sbjct: 243 GQAMVVDGGVTI 254


Lambda     K      H
   0.319    0.136    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 164
Number of extensions: 7
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 256
Length of database: 254
Length adjustment: 24
Effective length of query: 232
Effective length of database: 230
Effective search space:    53360
Effective search space used:    53360
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory