Aligments for a candidate for pgmA in Sphingomonas koreensis DSMZ 15582

GapMind for catabolism of small carbon sources

Aligments for a candidate for pgmA in Sphingomonas koreensis DSMZ 15582

Align phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent) (EC 5.4.2.2); phosphomannomutase (EC 5.4.2.8) (characterized)
to candidate Ga0059261_2456 Ga0059261_2456 Phosphomannomutase

Query= BRENDA::M1T754
         (460 letters)



>lcl|FitnessBrowser__Korea:Ga0059261_2456 Ga0059261_2456
           Phosphomannomutase
          Length = 460

 Score =  741 bits (1912), Expect = 0.0
 Identities = 360/460 (78%), Positives = 397/460 (86%)

Query: 1   MSHKFDPTTLREYDIRGIVGKTLNPADATAIGRGFGTLLRRAGGTRAAVGYDGRHSSPLL 60
           ++H+FDPT+LREYDIRGIVGKTL P DA AIGRGF TLLRRAGG R AVG DGR SSP L
Sbjct: 1   VTHRFDPTSLREYDIRGIVGKTLGPDDARAIGRGFATLLRRAGGHRVAVGRDGRVSSPEL 60

Query: 61  EAALVQGLIASGIDVVRVGLGPTPMLYYAEAVLEVDGGIMITGSHNPPDYNGFKMVFQHR 120
           EAALV+GL ASG DVVRVG+GPTPMLYYAEA+LEVDGGI ITGSHNP DYNGFKMVFQHR
Sbjct: 61  EAALVEGLTASGCDVVRVGMGPTPMLYYAEAILEVDGGIQITGSHNPGDYNGFKMVFQHR 120

Query: 121 PFFGEDILKIGTMAAEGDWEEGEGTVTNADIMDMYVDRLIAGYQGGAFKVAWDAGNGAAG 180
           PFFG+DI +IG +A  GDW+EGEGTV++ADI+D YV RL AGY GG F++ WD GNGAAG
Sbjct: 121 PFFGQDIQEIGKLAEAGDWDEGEGTVSDADILDDYVGRLFAGYAGGTFRIGWDTGNGAAG 180

Query: 181 PVIEKLVKFLPGEHHLLYTDVDGDFPNHHPDPTEEKNLVDLKALVAEKGLDFGIGFDGDG 240
           PVIEKLV+ LPGEHH L+TDVDG+FPNHHPDPTEEKNL DL+ LVAEK LDFG+ FDGDG
Sbjct: 181 PVIEKLVQLLPGEHHTLFTDVDGNFPNHHPDPTEEKNLADLRRLVAEKNLDFGLAFDGDG 240

Query: 241 DRIGAIDGKGRVVWGDQLLGILAEPVLKAVPGGTIIADVKTSQALYDRVAELGGKPLMWK 300
           DRIGAIDG+GRV+WGDQLL ILAEPVLK  PG TIIADVK SQ L+DRVAELGG+PLMWK
Sbjct: 241 DRIGAIDGEGRVIWGDQLLSILAEPVLKKAPGATIIADVKASQMLFDRVAELGGQPLMWK 300

Query: 301 TGHSLIKAKMKEVDSPLGGEMSGHIFFAWDYYGFDDAIYAAVRLMGAVRHSGKSLTELKD 360
           TGHSL+K KMKE  SPL GEMSGHIFFA DYYGFDDA YAAV+L+ AV   G SLTE++ 
Sbjct: 301 TGHSLVKTKMKETHSPLAGEMSGHIFFAQDYYGFDDAQYAAVQLINAVHLIGTSLTEIRG 360

Query: 361 AMPAMVNTPEMRFQVDESRKFAVIEEVLQRLEADGADINRTDGARVNTPDGWWLLRASNT 420
            MPA VNTPEMRFQVDESRKFAVI+EVL RL+A GAD+N TDGARVNTPDGWWLLRASNT
Sbjct: 361 NMPAFVNTPEMRFQVDESRKFAVIDEVLDRLKATGADVNDTDGARVNTPDGWWLLRASNT 420

Query: 421 QDVLVARAEAKDEAGLERLLKQIDTQLELSHIARGEQAGH 460
           QDVLVARAEA+ +  L+RLL QID QLE S + RG QA H
Sbjct: 421 QDVLVARAEARSQEALDRLLAQIDAQLEASGLKRGPQAAH 460


Lambda     K      H
   0.318    0.139    0.417 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 803
Number of extensions: 37
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 460
Length of database: 460
Length adjustment: 33
Effective length of query: 427
Effective length of database: 427
Effective search space:   182329
Effective search space used:   182329
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources