Align Aconitate hydratase A; ACN; Aconitase; (2R,3S)-2-methylisocitrate dehydratase; (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; IP210; Iron-responsive protein-like; IRP-like; Major iron-containing protein; MICP; Probable 2-methyl-cis-aconitate hydratase; RNA-binding protein; EC 4.2.1.3; EC 4.2.1.99 (characterized)
to candidate Ga0059261_3296 Ga0059261_3296 aconitate hydratase 1
Query= SwissProt::P37032 (891 letters) >FitnessBrowser__Korea:Ga0059261_3296 Length = 890 Score = 1149 bits (2972), Expect = 0.0 Identities = 585/891 (65%), Positives = 688/891 (77%), Gaps = 8/891 (0%) Query: 3 VGQDSLSTKSQLTVDGKTYNYYSLKEAENKHFKGINRLPYSLKVLLENLLRFEDGNTVTT 62 +GQDSL T+ LTV GK+Y+YYSL++A K ++RLP+S+KVLLEN+LRFEDG TVT Sbjct: 4 IGQDSLGTRETLTVGGKSYSYYSLEKAAAK-LGDVSRLPFSMKVLLENMLRFEDGVTVTP 62 Query: 63 KDIKAIADWLHNKTS-QHEIAFRPTRVLMQDFTGVPAVVDLAAMRTAIVKMGGNADKISP 121 +D +AI DW N + + EI +RP RVLMQDFTGVP VVDLAAMR AI K+GG+A KI+P Sbjct: 63 EDAQAIVDWQKNPNAPEREIQYRPARVLMQDFTGVPCVVDLAAMRDAITKLGGDAAKINP 122 Query: 122 LSPVDLVIDHSVMVDKFASADALEVNTKIEIERNKERYEFLRWGQKAFSNFQVVPPGTGI 181 PV LVIDHSVMVD+F + A E N ++E +RN ERY+FL+WG K+ NF+VVPPGTGI Sbjct: 123 QVPVHLVIDHSVMVDEFGTPKAFEENVELEYQRNMERYDFLKWGSKSLDNFKVVPPGTGI 182 Query: 182 CHQVNLEYLGKTVWNSEN-DGQLYAYPDTLVGTDSHTTMINGLGVLGWGVGGIEAEAAML 240 CHQVNLEY+ +W+S DG AYPDTLVGTDSHTTM+NGLGVLGWGVGGIEAEAAML Sbjct: 183 CHQVNLEYIADAIWSSTAADGTTVAYPDTLVGTDSHTTMVNGLGVLGWGVGGIEAEAAML 242 Query: 241 GQPVSMLIPEVIGFKLSGKLKEGITATDLVLTVTQMLRKKGVVGKFVEFYGPGLNDLPLA 300 GQPVSMLIPEV+GFKL+GKL+EGITATDLVLTVTQMLR +GVVG+FVEF+GPGL + LA Sbjct: 243 GQPVSMLIPEVVGFKLTGKLQEGITATDLVLTVTQMLRARGVVGRFVEFFGPGLATMTLA 302 Query: 301 DRATISNMAPEYGATCGFFPVDKETIKYLELTGRDKHTIALVEAYAKAQGMWYDKDNEEP 360 DRATI+NMAPEYGATCGFF +D +T+ Y+ LTGRD T+ LVEAY KAQGMW D +P Sbjct: 303 DRATIANMAPEYGATCGFFGIDDKTLDYMRLTGRDDDTVTLVEAYCKAQGMWRYDDMADP 362 Query: 361 VFTDSLHLDLGSVEPSLAGPKRPQDKVNLSSLPVEFNNFLIEVGKEKEKEKTFAVKNKDF 420 +FTD+L LD+ +V SLAGPKRPQD+V+L+ + FN L +V KE AV+ KD Sbjct: 363 IFTDTLELDMATVTASLAGPKRPQDRVSLNKVDEVFNGDLFKV-YGKENGHRVAVEGKDH 421 Query: 421 QMKHGHVVIAAITSCTNTSNPSVLMAAGLVAKKAIEKGLQRKPWVKSSLAPGSKVVTDYL 480 + G VVIAAITSCTNTSNPSVL+AAGLVA+KA KGL RKPWVK+SLAPGS+VVTDYL Sbjct: 422 DIGDGDVVIAAITSCTNTSNPSVLIAAGLVARKARAKGLTRKPWVKTSLAPGSQVVTDYL 481 Query: 481 RHAGLQTYLDQLGFNLVGYGCTTCIGNSGPLPDDISHCVAEHDLVVSSVLSGNRNFEGRV 540 AGL LD +GFNLVGYGCTTCIGNSGPL IS + +D+V +SVLSGNRNFEGRV Sbjct: 482 NKAGLSEDLDAIGFNLVGYGCTTCIGNSGPLAQPISDAINGNDIVAASVLSGNRNFEGRV 541 Query: 541 HPQVRANWLASPPLVVAYALCGTTCSDLSREPIGQDKEGNDVYLKDIWPSNEEIAAEV-A 599 P VRAN+LASPPLVVAYAL GT D+ PIG+ +G VYLKDIWP+NEE+ + A Sbjct: 542 SPDVRANFLASPPLVVAYALKGTVTEDMIETPIGEGTDG-PVYLKDIWPTNEEVQGVINA 600 Query: 600 KVSGTMFRKEYAEVFKGDAHWQAIQTSSGQTYEWNPDSTYIQHPPFFENLSLKPEPLKPI 659 + MF+ Y V+ GDAHWQ I TY W STYI +PP+F +++ P P+ I Sbjct: 601 NIDSEMFKSRYGNVYLGDAHWQKINVEGSATYSWPAASTYIANPPYFAGMTMTPAPVADI 660 Query: 660 KQAYVLALFGDSITTDHISPAGSIKASSPAGLYLKSKGVDEKDFNSYGSRRGNHEVMMRG 719 A LA+ GDSITTDHISPAGSIKA SPAG +L + V + DFNSYG+RRGN VM+RG Sbjct: 661 VDAKPLAILGDSITTDHISPAGSIKADSPAGKWLMERQVSKADFNSYGARRGNDNVMVRG 720 Query: 720 TFANIRIRNEMTPGQEGGVTRYVPTGETMSIYDAAMRYQENQQDLVIIAGKEYGTGSSRD 779 TFANIRIRNEM PG EGG+T Y GETM IYDAAMR++ + LVI+AGKEYGTGSSRD Sbjct: 721 TFANIRIRNEMVPGVEGGMTSY--AGETMPIYDAAMRHKADGTPLVIVAGKEYGTGSSRD 778 Query: 780 WAAKGTNLLGVKAVITESFERIHRSNLIGMGILPLQFKEGTTRKTLKLDGSERISIEISD 839 WAAKGTNLLGV+AVITESFERIHRSNL+GMG+LPLQF EG TR+TLKLDGSE +I Sbjct: 779 WAAKGTNLLGVRAVITESFERIHRSNLVGMGVLPLQFAEGVTRQTLKLDGSETFTITGVA 838 Query: 840 KLTPGAMVPVTIERQDGDIEKIETLCRIDTADELEYYKNGGILQYVLRKIS 890 L P V V + R DG E T CRIDT +ELEY+ NGGILQYVLR ++ Sbjct: 839 GLRPRQDVEVKLTRADGSSETFLTRCRIDTVNELEYFLNGGILQYVLRNLA 889 Lambda K H 0.316 0.134 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 2101 Number of extensions: 88 Number of successful extensions: 8 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 891 Length of database: 890 Length adjustment: 43 Effective length of query: 848 Effective length of database: 847 Effective search space: 718256 Effective search space used: 718256 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 56 (26.2 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory