Aligments for a candidate for bkdA in Sphingomonas koreensis DSMZ 15582

GapMind for catabolism of small carbon sources

Aligments for a candidate for bkdA in Sphingomonas koreensis DSMZ 15582

Align 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) (EC 1.2.4.4) (characterized)
to candidate Ga0059261_2273 Ga0059261_2273 Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Query= reanno::Smeli:SMc03201
         (410 letters)



>lcl|FitnessBrowser__Korea:Ga0059261_2273 Ga0059261_2273
           Pyruvate/2-oxoglutarate dehydrogenase complex,
           dehydrogenase (E1) component, eukaryotic type, alpha
           subunit
          Length = 429

 Score =  492 bits (1267), Expect = e-144
 Identities = 247/400 (61%), Positives = 290/400 (72%)

Query: 7   LSLHVPEPAVRPGDLPDFSNVKIPKAGSVPRPDVDVDPEEIRDLAYSIIRVLNREGEAVG 66
           LSLHVPEP  RPGD  DF+ V +P AG+  RPD   DP    +LAY+++RVL+  G+AVG
Sbjct: 13  LSLHVPEPKFRPGDAVDFTEVAVPPAGAQSRPDTAADPSSFHELAYTLVRVLDDNGQAVG 72

Query: 67  PWAGFLSDEELLTGLRHMMLLRAFDARMLMAQRQGKTSFYMQHLGEEAVSCAFRKALRKG 126
           PW   L  + L   L  M L+RAFD RM  AQRQGKTSFYM+  GEEAV+ A   AL   
Sbjct: 73  PWNPKLDPDTLRKMLHDMALVRAFDERMFRAQRQGKTSFYMKCTGEEAVAIAAAHALASD 132

Query: 127 DMNFPTYRQAGLLIADDYPMVEMMNQIFSNELDPCHGRQLPVMYTSKEHGFFTISGNLAT 186
           DM FP+YRQ GLLIA  Y +V+MMNQI+SN+ D   G+QLP+MY+SKE GFF+ISGNL T
Sbjct: 133 DMCFPSYRQQGLLIARGYSLVQMMNQIYSNKGDDLAGKQLPIMYSSKEKGFFSISGNLTT 192

Query: 187 QYVQAVGWAMASAIKNDTRIAAGWIGDGSTAESDFHSALVFASTYKAPVILNIVNNQWAI 246
           QY QAVGWAMASA K DTRIAA W G+GSTAE DFHSAL FA+ YKAPVILN+VNNQWAI
Sbjct: 193 QYPQAVGWAMASAAKGDTRIAATWCGEGSTAEGDFHSALTFATVYKAPVILNVVNNQWAI 252

Query: 247 STFQGIARGGSGTFAARGLGFGIPALRVDGNDYLAVYAVARWAAERARLNLGPTLIEYVT 306
           S+F G A   + TFAAR LG+GI  LRVDGND LAVYA   WAAERAR N GPTLIE+ T
Sbjct: 253 SSFSGFAGAEATTFAARALGYGIAGLRVDGNDALAVYAATLWAAERARTNQGPTLIEHFT 312

Query: 307 YRVGAHSTSDDPSAYRPKTESEAWPLGDPVLRLKKHLILRGAWSEERHAQAEAEIMDEVI 366
           YR   HSTSDDP+ YR   E  AWPLGDP+ RLK HLI  G W EERH + + E+ ++V 
Sbjct: 313 YRTEGHSTSDDPTQYRSAGEPTAWPLGDPIARLKAHLIAIGEWDEERHVEMDRELAEQVK 372

Query: 367 QAQKEAERHGTLHAGGRPSVRDIFEGVYAEMPPHIRRQRQ 406
            AQKEAE++G L  G    +  +F+GV+ EMP H+R QRQ
Sbjct: 373 VAQKEAEKNGILGHGLHQPLDSLFDGVFEEMPWHLREQRQ 412


Lambda     K      H
   0.320    0.135    0.410 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 588
Number of extensions: 16
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 410
Length of database: 429
Length adjustment: 32
Effective length of query: 378
Effective length of database: 397
Effective search space:   150066
Effective search space used:   150066
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources