Alignments for a candidate for mmsB in Sphingomonas koreensis DSMZ 15582

GapMind for catabolism of small carbon sources

Alignments for a candidate for mmsB in Sphingomonas koreensis DSMZ 15582

Align 3-hydroxyisobutyrate dehydrogenase; HIBADH; EC 1.1.1.31 (characterized)
to candidate Ga0059261_3686 Ga0059261_3686 3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31)

Query= SwissProt::P28811
         (298 letters)



>FitnessBrowser__Korea:Ga0059261_3686
          Length = 295

 Score =  281 bits (719), Expect = 1e-80
 Identities = 152/293 (51%), Positives = 186/293 (63%), Gaps = 4/293 (1%)

Query: 1   MTDIAFLGLGNMGGPMAANLLKAGHRVNVFDLQPKAVLGLVEQGAQGADSALQCCEGAEV 60
           M  + F+GLGNMGG MAANL KAGH V  FDL   A+      G    +SA    EGAE 
Sbjct: 1   MARVGFIGLGNMGGGMAANLAKAGHDVRAFDLSADALERAKAAGCLPVESAAAAAEGAEA 60

Query: 61  VISMLPAGQHVESLYLGDDGLLARVAGKPLLIDCSTIAPETARKVAEAAAAKGLTLLDAP 120
           VI+MLPAG+HVE +Y  +D +        +LIDCSTI   TAR+VA+AA AKGL  +DAP
Sbjct: 61  VITMLPAGKHVEQVY--EDSVFGAADTGAILIDCSTIDVATARRVADAAQAKGLAAVDAP 118

Query: 121 VSGGVGGARAGTLSFIVGGPAEGFARARPVLENMGRNIFHAGDHGAGQVAKICNNMLLGI 180
           VSGG+  A  GTL+F+VGG A  F RA   L  MG+ + HAG +GAGQ +KICNNMLLG 
Sbjct: 119 VSGGIAAAAGGTLTFMVGGDAAAFERAEVFLAAMGKAVIHAGGNGAGQASKICNNMLLGA 178

Query: 181 LMAGTAEALALGVKNGLDPAVLSEVMKQSSGGNWALNLYNPWPGVMPQAPASNGYAGGFQ 240
            M  T EAL L  K GLDP    ++   SSG  W+LN Y P PG+ PQ PA NGY GGF 
Sbjct: 179 TMVATCEALLLAQKLGLDPQTFYDIASVSSGACWSLNTYAPLPGMGPQTPADNGYQGGFA 238

Query: 241 VRLMNKDLGLALANAQAVQASTPLGALARNLFSLHAQADAEHEGLDFSSIQKL 293
             LM KDL LA+  A++  + TP+GA A  L++    ADA + G+DFS I K+
Sbjct: 239 AGLMLKDLKLAMEAAESTHSETPMGARAAELYTKF--ADAGNAGVDFSGIIKM 289


Lambda     K      H
   0.317    0.135    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 311
Number of extensions: 12
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 298
Length of database: 295
Length adjustment: 26
Effective length of query: 272
Effective length of database: 269
Effective search space:    73168
Effective search space used:    73168
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 48 (23.1 bits)

Align candidate Ga0059261_3686 Ga0059261_3686 (3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31))
to HMM TIGR01692 (mmsB: 3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR01692.hmm
# target sequence database:        /tmp/gapView.16143.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01692  [M=288]
Accession:   TIGR01692
Description: HIBADH: 3-hydroxyisobutyrate dehydrogenase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   9.6e-126  404.9  16.4   1.1e-125  404.8  16.4    1.0  1  lcl|FitnessBrowser__Korea:Ga0059261_3686  Ga0059261_3686 3-hydroxyisobutyr


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__Korea:Ga0059261_3686  Ga0059261_3686 3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31)
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  404.8  16.4  1.1e-125  1.1e-125       1     287 [.       6     290 ..       6     291 .. 0.99

  Alignments for each domain:
  == domain 1  score: 404.8 bits;  conditional E-value: 1.1e-125
                                 TIGR01692   1 fiGlGnmGgpmaanllkaghavrvfdlsakaveelveaGaqaaasaaeaveeadvvitmlPagkhvesv 69 
                                               fiGlGnmGg+maanl+kagh vr+fdlsa+a+e++++aG+   +saa+a+e+a++vitmlPagkhve+v
  lcl|FitnessBrowser__Korea:Ga0059261_3686   6 FIGLGNMGGGMAANLAKAGHDVRAFDLSADALERAKAAGCLPVESAAAAAEGAEAVITMLPAGKHVEQV 74 
                                               9******************************************************************** PP

                                 TIGR01692  70 yagdegilakvkkksllidsstidvdsarkvaeeaaakGavfldaPvsGGvggaraGtltfmvGgeaee 138
                                               y  ++ +   +  +++lid+stidv +ar+va++a+akG+  +daPvsGG+++a+ GtltfmvGg a++
  lcl|FitnessBrowser__Korea:Ga0059261_3686  75 Y--EDSVFGAADTGAILIDCSTIDVATARRVADAAQAKGLAAVDAPVSGGIAAAAGGTLTFMVGGDAAA 141
                                               *..59**************************************************************** PP

                                 TIGR01692 139 faraepvleamgknivhaGdaGaGqaakiCnnlllgismiGvaealalgeklGldpkvlaeilskssGr 207
                                               f+rae  l+amgk ++haG++GaGqa+kiCnn+llg +m+ ++eal l++klGldp+++ +i+s ssG 
  lcl|FitnessBrowser__Korea:Ga0059261_3686 142 FERAEVFLAAMGKAVIHAGGNGAGQASKICNNMLLGATMVATCEALLLAQKLGLDPQTFYDIASVSSGA 210
                                               ********************************************************************* PP

                                 TIGR01692 208 cWsldtynPvPGvveqaPasngykgGfatalmlkdlglaqeaaksvkasvllGalarelyslfaskgag 276
                                               cWsl+ty+P+PG+ +q+Pa+ngy+gGfa+ lmlkdl+la eaa+s+++++++Ga a ely+ fa++g  
  lcl|FitnessBrowser__Korea:Ga0059261_3686 211 CWSLNTYAPLPGMGPQTPADNGYQGGFAAGLMLKDLKLAMEAAESTHSETPMGARAAELYTKFADAGNA 279
                                               ********************************************************************* PP

                                 TIGR01692 277 kkdfsavikll 287
                                               + dfs++ik+l
  lcl|FitnessBrowser__Korea:Ga0059261_3686 280 GVDFSGIIKML 290
                                               ********986 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (288 nodes)
Target sequences:                          1  (295 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.01
# Mc/sec: 7.38
//
[ok]

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources