Protein BWI76_RS06035 in Klebsiella michiganensis M5al
Annotation: FitnessBrowser__Koxy:BWI76_RS06035
Length: 348 amino acids
Source: Koxy in FitnessBrowser
Candidate for 15 steps in catabolism of small carbon sources
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|
| L-arabinose catabolism | araV | med | AraV, component of Arabinose, fructose, xylose porter (characterized) | 41% | 78% | 221.1 | CP4-6 prophage; ABC transporter ATP-binding protein AfuC | 94% | 656.4 |
| D-fructose catabolism | araV | med | AraV, component of Arabinose, fructose, xylose porter (characterized) | 41% | 78% | 221.1 | CP4-6 prophage; ABC transporter ATP-binding protein AfuC | 94% | 656.4 |
| sucrose catabolism | araV | med | AraV, component of Arabinose, fructose, xylose porter (characterized) | 41% | 78% | 221.1 | CP4-6 prophage; ABC transporter ATP-binding protein AfuC | 94% | 656.4 |
| D-xylose catabolism | araV | med | AraV, component of Arabinose, fructose, xylose porter (characterized) | 41% | 78% | 221.1 | CP4-6 prophage; ABC transporter ATP-binding protein AfuC | 94% | 656.4 |
| putrescine catabolism | potA | lo | Spermidine/putrescine import ATP-binding protein PotA, component of The spermidine/putrescine uptake porter, PotABCD (characterized) | 39% | 89% | 245.4 | CP4-6 prophage; ABC transporter ATP-binding protein AfuC | 94% | 656.4 |
| D-cellobiose catabolism | glcV | lo | monosaccharide-transporting ATPase (EC 3.6.3.17) (characterized) | 35% | 88% | 208 | CP4-6 prophage; ABC transporter ATP-binding protein AfuC | 94% | 656.4 |
| D-galactose catabolism | glcV | lo | monosaccharide-transporting ATPase (EC 3.6.3.17) (characterized) | 35% | 88% | 208 | CP4-6 prophage; ABC transporter ATP-binding protein AfuC | 94% | 656.4 |
| D-glucose catabolism | glcV | lo | monosaccharide-transporting ATPase (EC 3.6.3.17) (characterized) | 35% | 88% | 208 | CP4-6 prophage; ABC transporter ATP-binding protein AfuC | 94% | 656.4 |
| lactose catabolism | glcV | lo | monosaccharide-transporting ATPase (EC 3.6.3.17) (characterized) | 35% | 88% | 208 | CP4-6 prophage; ABC transporter ATP-binding protein AfuC | 94% | 656.4 |
| D-maltose catabolism | glcV | lo | monosaccharide-transporting ATPase (EC 3.6.3.17) (characterized) | 35% | 88% | 208 | CP4-6 prophage; ABC transporter ATP-binding protein AfuC | 94% | 656.4 |
| D-mannose catabolism | glcV | lo | monosaccharide-transporting ATPase (EC 3.6.3.17) (characterized) | 35% | 88% | 208 | CP4-6 prophage; ABC transporter ATP-binding protein AfuC | 94% | 656.4 |
| sucrose catabolism | glcV | lo | monosaccharide-transporting ATPase (EC 3.6.3.17) (characterized) | 35% | 88% | 208 | CP4-6 prophage; ABC transporter ATP-binding protein AfuC | 94% | 656.4 |
| trehalose catabolism | glcV | lo | monosaccharide-transporting ATPase (EC 3.6.3.17) (characterized) | 35% | 88% | 208 | CP4-6 prophage; ABC transporter ATP-binding protein AfuC | 94% | 656.4 |
| glycerol catabolism | glpT | lo | GlpT, component of Glycerol uptake porter, GlpSTPQV (characterized) | 34% | 97% | 196.4 | CP4-6 prophage; ABC transporter ATP-binding protein AfuC | 94% | 656.4 |
| L-proline catabolism | opuBA | lo | BusAA, component of Uptake system for glycine-betaine (high affinity) and proline (low affinity) (OpuAA-OpuABC) or BusAA-ABC of Lactococcus lactis). BusAA, the ATPase subunit, has a C-terminal tandem cystathionine β-synthase (CBS) domain which is the cytoplasmic K+ sensor for osmotic stress (osmotic strength)while the BusABC subunit has the membrane and receptor domains fused to each other (Biemans-Oldehinkel et al., 2006; Mahmood et al., 2006; Gul et al. 2012). An N-terminal amphipathic α-helix of OpuA is necessary for high activity but is not critical for biogenesis or the ionic regulation of transport (characterized) | 37% | 62% | 184.9 | CP4-6 prophage; ABC transporter ATP-binding protein AfuC | 94% | 656.4 |
Sequence Analysis Tools
View BWI76_RS06035 at FitnessBrowser
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
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Sequence
MSQKNFVELRNVTKRFGSNMVIDNINLTIPQGQMVTLLGPSGCGKTTILRLVAGLEKPSE
GQIYIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGDNVGYGLKMLGVPRGDVKARVKEA
LAMVDLEGFEDRYVDQISGGQQQRVALARALILKPKVLLFDEPLSNLDANLRRSMRDKIR
ELQKQFDITSLYVTHDQSEAFAVSDTVLVMNKGHIMQIGSPQELYRQPASRFMASFMGDA
NLFPAAFSEDFVDIYGYRLPRPPHFAAHGEGSVGVRPEAITLSDRGEESQRCTIRHVAYM
GPQYEVTVEWHGQEILLQVNATRLQPDVGEHYYLEIHPYGMFVLADAG
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory