GapMind for catabolism of small carbon sources

 

Protein BWI76_RS14865 in Klebsiella michiganensis M5al

Annotation: BWI76_RS14865 ribose ABC transporter permease

Length: 339 amino acids

Source: Koxy in FitnessBrowser

Candidate for 34 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
D-ribose catabolism rbsC hi Ribose import permease protein RbsC (characterized) 52% 97% 292.4 RbsC, component of The probable autoinducer-2 (AI-2;, a furanosyl borate diester: 3aS,6S,6aR)-2,2,6,6a-tetrahydroxy-3a-methyltetrahydrofuro[3,2-d][1,3,2]dioxaborolan-2-uide) uptake porter (Shao et al., 2007) (50-70% identical to RbsABC of E. coli; TC# 3.A.1.2.1) 48% 277.7
xylitol catabolism PS417_12060 med ABC transporter permease; SubName: Full=Monosaccharide ABC transporter membrane protein, CUT2 family; SubName: Full=Sugar ABC transporter permease (characterized, see rationale) 44% 99% 278.5 Ribose import permease protein RbsC 52% 292.4
D-fructose catabolism frcC med Ribose ABC transport system, permease protein RbsC (characterized, see rationale) 49% 89% 271.2 Ribose import permease protein RbsC 52% 292.4
sucrose catabolism frcC med Ribose ABC transport system, permease protein RbsC (characterized, see rationale) 49% 89% 271.2 Ribose import permease protein RbsC 52% 292.4
D-mannose catabolism HSERO_RS03645 med ABC-type sugar transport system, permease component protein (characterized, see rationale) 45% 92% 269.2 Ribose import permease protein RbsC 52% 292.4
D-cellobiose catabolism mglC med Putative beta-xyloside ABC transporter, permease component, component of Glucose porter. Also bind xylose (Boucher and Noll 2011). Induced by glucose (Frock et al. 2012). Directly regulated by glucose-responsive regulator GluR (characterized) 44% 97% 247.7 Ribose import permease protein RbsC 52% 292.4
D-glucose catabolism mglC med Putative beta-xyloside ABC transporter, permease component, component of Glucose porter. Also bind xylose (Boucher and Noll 2011). Induced by glucose (Frock et al. 2012). Directly regulated by glucose-responsive regulator GluR (characterized) 44% 97% 247.7 Ribose import permease protein RbsC 52% 292.4
lactose catabolism mglC med Putative beta-xyloside ABC transporter, permease component, component of Glucose porter. Also bind xylose (Boucher and Noll 2011). Induced by glucose (Frock et al. 2012). Directly regulated by glucose-responsive regulator GluR (characterized) 44% 97% 247.7 Ribose import permease protein RbsC 52% 292.4
D-maltose catabolism mglC med Putative beta-xyloside ABC transporter, permease component, component of Glucose porter. Also bind xylose (Boucher and Noll 2011). Induced by glucose (Frock et al. 2012). Directly regulated by glucose-responsive regulator GluR (characterized) 44% 97% 247.7 Ribose import permease protein RbsC 52% 292.4
sucrose catabolism mglC med Putative beta-xyloside ABC transporter, permease component, component of Glucose porter. Also bind xylose (Boucher and Noll 2011). Induced by glucose (Frock et al. 2012). Directly regulated by glucose-responsive regulator GluR (characterized) 44% 97% 247.7 Ribose import permease protein RbsC 52% 292.4
trehalose catabolism mglC med Putative beta-xyloside ABC transporter, permease component, component of Glucose porter. Also bind xylose (Boucher and Noll 2011). Induced by glucose (Frock et al. 2012). Directly regulated by glucose-responsive regulator GluR (characterized) 44% 97% 247.7 Ribose import permease protein RbsC 52% 292.4
D-xylose catabolism xylH med Putative beta-xyloside ABC transporter, permease component, component of Glucose porter. Also bind xylose (Boucher and Noll 2011). Induced by glucose (Frock et al. 2012). Directly regulated by glucose-responsive regulator GluR (characterized) 44% 97% 247.7 Ribose import permease protein RbsC 52% 292.4
L-fucose catabolism HSERO_RS05255 med ABC-type sugar transport system, permease component protein (characterized, see rationale) 46% 87% 245.4 Ribose import permease protein RbsC 52% 292.4
myo-inositol catabolism PS417_11895 med Inositol transport system permease protein (characterized) 41% 99% 240.4 Ribose import permease protein RbsC 52% 292.4
myo-inositol catabolism iatP med Inositol ABC transport system, permease protein IatP, component of The myoinositol (high affinity)/ D-ribose (low affinity) transporter IatP/IatA/IbpA. The structure of IbpA with myoinositol bound has been solved (characterized) 41% 97% 232.3 Ribose import permease protein RbsC 52% 292.4
D-xylose catabolism xylF_Tm med ABC-type transporter, integral membrane subunit, component of Xylose porter (Nanavati et al. 2006). Regulated by xylose-responsive regulator XylR (characterized) 42% 95% 227.3 Ribose import permease protein RbsC 52% 292.4
D-mannose catabolism frcC med Fructose import permease protein FrcC (characterized) 42% 84% 211.5 Ribose import permease protein RbsC 52% 292.4
D-ribose catabolism frcC med Fructose import permease protein FrcC (characterized) 42% 84% 211.5 Ribose import permease protein RbsC 52% 292.4
L-arabinose catabolism araH lo L-arabinose ABC transporter, permease protein AraH (characterized) 38% 95% 229.9 Ribose import permease protein RbsC 52% 292.4
D-galactose catabolism BPHYT_RS16925 lo Monosaccharide-transporting ATPase; EC 3.6.3.17 (characterized, see rationale) 36% 95% 223.4 Ribose import permease protein RbsC 52% 292.4
D-galactose catabolism ytfT lo Galactofuranose transporter permease protein YtfT (characterized) 37% 96% 207.6 Ribose import permease protein RbsC 52% 292.4
D-galactose catabolism mglC lo MglC aka B2148, component of Galactose/glucose (methyl galactoside) porter (characterized) 38% 96% 201.1 Ribose import permease protein RbsC 52% 292.4
L-rhamnose catabolism rhaP lo RhaP, component of Rhamnose porter (Richardson et al., 2004) (Transport activity is dependent on rhamnokinase (RhaK; AAQ92412) activity (Richardson and Oresnik, 2007) This could be an example of group translocation!) (characterized) 35% 90% 192.2 Ribose import permease protein RbsC 52% 292.4
L-arabinose catabolism araWsh lo Inner-membrane translocator (characterized, see rationale) 36% 79% 187.6 Ribose import permease protein RbsC 52% 292.4
L-rhamnose catabolism rhaQ lo RhaQ (characterized, see rationale) 31% 92% 181.8 Ribose import permease protein RbsC 52% 292.4
D-galactose catabolism yjtF lo Inner membrane ABC transporter permease protein YjfF (characterized) 38% 91% 176.4 Ribose import permease protein RbsC 52% 292.4
D-fructose catabolism fruF lo Fructose import permease protein FruF (characterized) 33% 91% 175.3 Ribose import permease protein RbsC 52% 292.4
sucrose catabolism fruF lo Fructose import permease protein FruF (characterized) 33% 91% 175.3 Ribose import permease protein RbsC 52% 292.4
D-fructose catabolism fruG lo Fructose import permease protein FruG (characterized) 34% 96% 172.9 Ribose import permease protein RbsC 52% 292.4
sucrose catabolism fruG lo Fructose import permease protein FruG (characterized) 34% 96% 172.9 Ribose import permease protein RbsC 52% 292.4
L-arabinose catabolism araZsh lo Inner-membrane translocator (characterized, see rationale) 35% 98% 165.6 Ribose import permease protein RbsC 52% 292.4
2'-deoxyinosine catabolism H281DRAFT_01115 lo deoxynucleoside transporter, permease component 1 (characterized) 32% 93% 147.5 Ribose import permease protein RbsC 52% 292.4
L-arabinose catabolism gguB lo GguB aka ATU2346 aka AGR_C_4262, component of Multiple sugar (arabinose, xylose, galactose, glucose, fucose) putative porter (characterized) 31% 83% 134.8 Ribose import permease protein RbsC 52% 292.4
D-galactose catabolism gguB lo GguB aka ATU2346 aka AGR_C_4262, component of Multiple sugar (arabinose, xylose, galactose, glucose, fucose) putative porter (characterized) 31% 83% 134.8 Ribose import permease protein RbsC 52% 292.4

Sequence Analysis Tools

View BWI76_RS14865 at FitnessBrowser

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search PFam (including for weak hits, up to E = 1)

Predict protein localization: PSORTb (Gram negative bacteria)

Predict transmembrane helices: TMHMM

Check the SEED with FIGfam search

Fitness BLAST: loading...

Sequence

MDNNKPVTELPVKAPFDFAKWWDRVGILIVLLVLLILMSTFAPNFNRVDNLLNIARSISV
NAILAAGMTFVILTSGIDLSVGSIVAVSGVVSVVAAMAGIPAPLAILAGVGVGALCGLLN
GVLTAYLALAPFIVTLGTMTFLRGMAYTITEGQPIVSSSLSFRELGNGYLIGIPIPVIIM
LVVYLLAWFILERTRFGRHIYAVGGNAQAARLAGVRVKRVLAAVYMIAGVCAGLAGIIFA
ARVISAQPTAGTGYELDAIAAVVLGGTSLAGGRGRIIGTLIGSIILGVLSTGLILLSVPF
FTQLLIKGIVIILAVAIDGLKQHPELFTFWRKKEIARPR

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory