Protein BWI76_RS19555 in Klebsiella michiganensis M5al
Annotation: FitnessBrowser__Koxy:BWI76_RS19555
Length: 619 amino acids
Source: Koxy in FitnessBrowser
Candidate for 19 steps in catabolism of small carbon sources
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|
| D-cellobiose catabolism | bglF | hi | β-Glucoside (salicin, arbutin, cellobiose, etc) group translocator, BglF (characterized) | 56% | 100% | 712.2 | PTS beta-glucoside transporter, EIIBCA of 672 aas and 12 predicted TMSs | 41% | 513.8 |
| D-cellobiose catabolism | bglG | hi | β-Glucoside (salicin, arbutin, cellobiose, etc) group translocator, BglF (characterized) | 56% | 100% | 712.2 | Aryl β-glucoside porter, IIBCA (BglP; SytA) (35% identical to 4.A.1.2.2) | 39% | 458.4 |
| D-glucose catabolism | bglF | hi | β-Glucoside (salicin, arbutin, cellobiose, etc) group translocator, BglF (characterized) | 56% | 100% | 712.2 | PTS beta-glucoside transporter, EIIBCA of 672 aas and 12 predicted TMSs | 41% | 513.8 |
| lactose catabolism | bglF | hi | β-Glucoside (salicin, arbutin, cellobiose, etc) group translocator, BglF (characterized) | 56% | 100% | 712.2 | PTS beta-glucoside transporter, EIIBCA of 672 aas and 12 predicted TMSs | 41% | 513.8 |
| D-maltose catabolism | bglF | hi | β-Glucoside (salicin, arbutin, cellobiose, etc) group translocator, BglF (characterized) | 56% | 100% | 712.2 | PTS beta-glucoside transporter, EIIBCA of 672 aas and 12 predicted TMSs | 41% | 513.8 |
| sucrose catabolism | bglF | hi | β-Glucoside (salicin, arbutin, cellobiose, etc) group translocator, BglF (characterized) | 56% | 100% | 712.2 | PTS beta-glucoside transporter, EIIBCA of 672 aas and 12 predicted TMSs | 41% | 513.8 |
| trehalose catabolism | bglF | hi | β-Glucoside (salicin, arbutin, cellobiose, etc) group translocator, BglF (characterized) | 56% | 100% | 712.2 | PTS beta-glucoside transporter, EIIBCA of 672 aas and 12 predicted TMSs | 41% | 513.8 |
| N-acetyl-D-glucosamine catabolism | nagEIIA | med | Putative phosphotransferase enzyme IIA component YpqE (characterized, see rationale) | 42% | 88% | 131 | β-Glucoside (salicin, arbutin, cellobiose, etc) group translocator, BglF | 56% | 712.2 |
| D-glucosamine (chitosamine) catabolism | nagEIIA | med | Putative phosphotransferase enzyme IIA component YpqE (characterized, see rationale) | 42% | 88% | 131 | β-Glucoside (salicin, arbutin, cellobiose, etc) group translocator, BglF | 56% | 712.2 |
| D-maltose catabolism | malEIIA | med | Putative phosphotransferase enzyme IIA component YpqE (characterized, see rationale) | 42% | 88% | 131 | β-Glucoside (salicin, arbutin, cellobiose, etc) group translocator, BglF | 56% | 712.2 |
| trehalose catabolism | treEIIA | med | Putative phosphotransferase enzyme IIA component YpqE (characterized, see rationale) | 42% | 88% | 131 | β-Glucoside (salicin, arbutin, cellobiose, etc) group translocator, BglF | 56% | 712.2 |
| sucrose catabolism | ptsS | lo | The sucrose porter, PtsS (regulated by SugR which also regulates other enzymes II) (characterized) | 32% | 99% | 335.1 | β-Glucoside (salicin, arbutin, cellobiose, etc) group translocator, BglF | 56% | 712.2 |
| sucrose catabolism | sacP | lo | Negative regulator of SacY activity; PTS system sac EIIBC component; EC 2.7.1.- (characterized) | 31% | 100% | 219.2 | β-Glucoside (salicin, arbutin, cellobiose, etc) group translocator, BglF | 56% | 712.2 |
| D-cellobiose catabolism | crr | lo | glucose-specific phosphotransferase enzyme IIA component; EC 2.7.1.- (characterized) | 38% | 88% | 126.7 | β-Glucoside (salicin, arbutin, cellobiose, etc) group translocator, BglF | 56% | 712.2 |
| D-glucose catabolism | crr | lo | glucose-specific phosphotransferase enzyme IIA component; EC 2.7.1.- (characterized) | 38% | 88% | 126.7 | β-Glucoside (salicin, arbutin, cellobiose, etc) group translocator, BglF | 56% | 712.2 |
| lactose catabolism | crr | lo | glucose-specific phosphotransferase enzyme IIA component; EC 2.7.1.- (characterized) | 38% | 88% | 126.7 | β-Glucoside (salicin, arbutin, cellobiose, etc) group translocator, BglF | 56% | 712.2 |
| D-maltose catabolism | crr | lo | glucose-specific phosphotransferase enzyme IIA component; EC 2.7.1.- (characterized) | 38% | 88% | 126.7 | β-Glucoside (salicin, arbutin, cellobiose, etc) group translocator, BglF | 56% | 712.2 |
| sucrose catabolism | crr | lo | glucose-specific phosphotransferase enzyme IIA component; EC 2.7.1.- (characterized) | 38% | 88% | 126.7 | β-Glucoside (salicin, arbutin, cellobiose, etc) group translocator, BglF | 56% | 712.2 |
| trehalose catabolism | crr | lo | glucose-specific phosphotransferase enzyme IIA component; EC 2.7.1.- (characterized) | 38% | 88% | 126.7 | β-Glucoside (salicin, arbutin, cellobiose, etc) group translocator, BglF | 56% | 712.2 |
Sequence Analysis Tools
View BWI76_RS19555 at FitnessBrowser
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
Fitness BLAST: loading...
Sequence
MEYKALAQDILTRVGGRENIVSLVHCATRLRFKLKDSKKADAEGLKANPGVIMVVESGGQ
FQVVIGNHVHDVWLAVRSEAGLTDDSEPVAVKGEKVSLIGQLIDIVSGIFTPFIGVLAAS
GILKGLLALAVVCGWLTTQQATYKIWFAASDALFFFFPLFLGYTAGKKFGGNPFITMVIG
GALTHPLMIQAFDASMAPGAATEYFLGIPVTFINYSSSVIPIILASWVSCWIEKRSNAIL
PSSMKNFFTPAICLAVVVPLTFLIIGPVATWLSQLLANGYQLIYQVAPWLAGAAMGALWQ
VCVIFGLHWGLIPLMINNLAVLGHDSMMPMLLPAVMGQVGAALGIFLRTRDARQKVLAGS
AVSAGIFGVTEPAIYGLNLPLRRPFIFGCVAGAIGGAMVGFSDSHVYSFGFGNIFTLAQM
IPPEGIDATVWGGAAGMFASLIIACVLTLVAGLPRSSAEQAAVVVAPASENDILAPMTGS
VLALDQVPDSTFASGLLGQGVAIIPSVGKVIAPFSGEVASIFQTKHAIGLLSDSGIELLI
HVGIDTVKLDGAPFTAHVKEGDKIKAGDLLLEFDRQAILDAGYDLATPIIISNSDDFRTL
DMVSASAVDAGQPLLSVSR
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory