GapMind for catabolism of small carbon sources

 

Protein BWI76_RS22065 in Klebsiella michiganensis M5al

Annotation: FitnessBrowser__Koxy:BWI76_RS22065

Length: 400 amino acids

Source: Koxy in FitnessBrowser

Candidate for 5 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-proline catabolism proV hi Glycine betaine/proline betaine transport system ATP-binding protein ProV (characterized) 93% 100% 731.5 OtaA, component of The salt-induced glycine betaine OtaABC transporter 51% 380.9
L-proline catabolism opuBA med BusAA, component of Uptake system for glycine-betaine (high affinity) and proline (low affinity) (OpuAA-OpuABC) or BusAA-ABC of Lactococcus lactis). BusAA, the ATPase subunit, has a C-terminal tandem cystathionine β-synthase (CBS) domain which is the cytoplasmic K+ sensor for osmotic stress (osmotic strength)while the BusABC subunit has the membrane and receptor domains fused to each other (Biemans-Oldehinkel et al., 2006; Mahmood et al., 2006; Gul et al. 2012). An N-terminal amphipathic α-helix of OpuA is necessary for high activity but is not critical for biogenesis or the ionic regulation of transport (characterized) 47% 96% 354.4 Glycine betaine/proline betaine transport system ATP-binding protein ProV 93% 731.5
L-histidine catabolism hutV med ABC transporter for L-Histidine, ATPase component (characterized) 58% 95% 304.7 Glycine betaine/proline betaine transport system ATP-binding protein ProV 93% 731.5
L-proline catabolism hutV med HutV aka HISV aka R02702 aka SMC00670, component of Uptake system for hisitidine, proline, proline-betaine and glycine-betaine (characterized) 56% 95% 294.7 Glycine betaine/proline betaine transport system ATP-binding protein ProV 93% 731.5
L-tryptophan catabolism ecfA1 lo Energy-coupling factor transporter ATP-binding protein EcfA1; Short=ECF transporter A component EcfA; EC 7.-.-.- (characterized, see rationale) 37% 90% 150.2 Glycine betaine/proline betaine transport system ATP-binding protein ProV 93% 731.5

Sequence Analysis Tools

View BWI76_RS22065 at FitnessBrowser

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MAIKLEIKNLYKIFGEHPQRAFKYIDKGLSKEQILEKTGLSLGVKNASLTIEEGEIFVIM
GLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELREVRRKKIAMVFQSFALMPH
MTVLDNAGFGMELAGVPTQERQEKALEALRQVGLENYAHAYPDELSGGMRQRVGLARALA
INPDILLMDEAFSALDPLIRTEMQDELTKLQARHQRTVVFISHDLDEAMRIGDRIAIMQN
GEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSAKDIARRSPVGLIRKTPGFGPRSAL
KLLQDEDREYGYVIERGNKFVGIISIDSLKAALSEGQGIDAALIDSPQAVDAQTPLSELL
SHVGHAPCAVPVIDEGQQYVGIISKRMLLQALDREGANNG

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory