GapMind for catabolism of small carbon sources

 

Protein BWI76_RS22070 in Klebsiella michiganensis M5al

Annotation: BWI76_RS22070 proline/betaine ABC transporter permease ProW

Length: 354 amino acids

Source: Koxy in FitnessBrowser

Candidate for 4 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-proline catabolism proW hi Glycine betaine/proline betaine transport system permease protein ProW (characterized) 91% 100% 649 Glycine betaine transport system permease protein OpuAB 47% 256.9
L-histidine catabolism hutW med ABC transporter for L-Histidine, permease component (characterized) 48% 94% 241.5 Glycine betaine/proline betaine transport system permease protein ProW 91% 649.0
L-proline catabolism hutW med HutW aka HISW, component of Uptake system for hisitidine, proline, proline-betaine and glycine-betaine (characterized) 44% 99% 234.6 Glycine betaine/proline betaine transport system permease protein ProW 91% 649.0
L-proline catabolism opuBB lo BusAB aka OPUABC, component of Uptake system for glycine-betaine (high affinity) and proline (low affinity) (OpuAA-OpuABC) or BusAA-ABC of Lactococcus lactis). BusAA, the ATPase subunit, has a C-terminal tandem cystathionine β-synthase (CBS) domain which is the cytoplasmic K+ sensor for osmotic stress (osmotic strength)while the BusABC subunit has the membrane and receptor domains fused to each other (Biemans-Oldehinkel et al., 2006; Mahmood et al., 2006; Gul et al. 2012). An N-terminal amphipathic α-helix of OpuA is necessary for high activity but is not critical for biogenesis or the ionic regulation of transport (characterized) 46% 53% 242.7 Glycine betaine/proline betaine transport system permease protein ProW 91% 649.0

Sequence Analysis Tools

View BWI76_RS22070 at FitnessBrowser

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search PFam (including for weak hits, up to E = 1)

Predict protein localization: PSORTb (Gram negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the SEED with FIGfam search

Fitness BLAST: loading...

Sequence

MADQSNPWDTAPAADSAAQSADAWGTSTPAPTNGGSADWLSSAPAPQPEHFNIMDPFHNT
LIPLDSWVTHGIDWVVMHFRPLFQGIRVPVDYILSAFQQLLLGMPAPVAIIIFSLIAWQI
SSVGMGAATLISLIAIGAIGAWSQAMVTLALVLTALLFCMLIGLPLGIWLARSPRAAKII
RPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPIVRLTILGINQVPADLIEA
SRSFGASPRQLLFKVQLPLAMPTIMAGVNQTLMLALSMVVIASMIAVGGLGQMVLRGIGR
LDMGLATVGGVGIVILAIILDRLTQAVGRDSRSRGNRRWYATGPLGLITRPFCK

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory