Alignments for a candidate for adh in Klebsiella michiganensis M5al

GapMind for catabolism of small carbon sources

Alignments for a candidate for adh in Klebsiella michiganensis M5al

Align acetaldehyde dehydrogenase (EC 1.2.1.3) (characterized)
to candidate BWI76_RS21985 BWI76_RS21985 aldehyde dehydrogenase

Query= reanno::Koxy:BWI76_RS21985
         (506 letters)



>FitnessBrowser__Koxy:BWI76_RS21985
          Length = 506

 Score = 1020 bits (2638), Expect = 0.0
 Identities = 506/506 (100%), Positives = 506/506 (100%)

Query: 1   MRYAHPGTPGALVALKSAYGNFIDGKFVEPIGGEFFMNTSPVDGSNIGQFPRSDAKDIDF 60
           MRYAHPGTPGALVALKSAYGNFIDGKFVEPIGGEFFMNTSPVDGSNIGQFPRSDAKDIDF
Sbjct: 1   MRYAHPGTPGALVALKSAYGNFIDGKFVEPIGGEFFMNTSPVDGSNIGQFPRSDAKDIDF 60

Query: 61  ALDAAHRAADAWGKTSAQHRANLLLQVADRIEANLEYLAVAESWDNGKPIRETLNADLPL 120
           ALDAAHRAADAWGKTSAQHRANLLLQVADRIEANLEYLAVAESWDNGKPIRETLNADLPL
Sbjct: 61  ALDAAHRAADAWGKTSAQHRANLLLQVADRIEANLEYLAVAESWDNGKPIRETLNADLPL 120

Query: 121 AVDHFRYFAGCLRAQEGSTAEIDETTVAYHFHEPLGVVGQIIPWNFPLLMAAWKLAPALA 180
           AVDHFRYFAGCLRAQEGSTAEIDETTVAYHFHEPLGVVGQIIPWNFPLLMAAWKLAPALA
Sbjct: 121 AVDHFRYFAGCLRAQEGSTAEIDETTVAYHFHEPLGVVGQIIPWNFPLLMAAWKLAPALA 180

Query: 181 AGNCVVLKPAEQTPLSITLLLEIIGDLFPAGVLNVVQGFGKEAGEALATSKRIAKIAFTG 240
           AGNCVVLKPAEQTPLSITLLLEIIGDLFPAGVLNVVQGFGKEAGEALATSKRIAKIAFTG
Sbjct: 181 AGNCVVLKPAEQTPLSITLLLEIIGDLFPAGVLNVVQGFGKEAGEALATSKRIAKIAFTG 240

Query: 241 STPVGRHIMACAAENIIPCTVELGGKSPNIYFADVMEGEEEYIEKAVEGLVLGFFNQGEV 300
           STPVGRHIMACAAENIIPCTVELGGKSPNIYFADVMEGEEEYIEKAVEGLVLGFFNQGEV
Sbjct: 241 STPVGRHIMACAAENIIPCTVELGGKSPNIYFADVMEGEEEYIEKAVEGLVLGFFNQGEV 300

Query: 301 CTCPSRALIQESIYEPFMARVMDKVAQIRRGDPFDTDTMIGAQASRQQFDKILSYIKIAR 360
           CTCPSRALIQESIYEPFMARVMDKVAQIRRGDPFDTDTMIGAQASRQQFDKILSYIKIAR
Sbjct: 301 CTCPSRALIQESIYEPFMARVMDKVAQIRRGDPFDTDTMIGAQASRQQFDKILSYIKIAR 360

Query: 361 DEGGKILTGGERASISAELDNGFYIQPTLIQGRNDMRSFQEEIFGPVIGVTTFKDEAEAL 420
           DEGGKILTGGERASISAELDNGFYIQPTLIQGRNDMRSFQEEIFGPVIGVTTFKDEAEAL
Sbjct: 361 DEGGKILTGGERASISAELDNGFYIQPTLIQGRNDMRSFQEEIFGPVIGVTTFKDEAEAL 420

Query: 421 SIANQTQFGLGAGVWTRDTNLAYRMGRGIKAGRVWTNCYHIYPAHAAFGGYKQSGVGRET 480
           SIANQTQFGLGAGVWTRDTNLAYRMGRGIKAGRVWTNCYHIYPAHAAFGGYKQSGVGRET
Sbjct: 421 SIANQTQFGLGAGVWTRDTNLAYRMGRGIKAGRVWTNCYHIYPAHAAFGGYKQSGVGRET 480

Query: 481 HKMALNAYQQTKNLLVSYGTAPLGLF 506
           HKMALNAYQQTKNLLVSYGTAPLGLF
Sbjct: 481 HKMALNAYQQTKNLLVSYGTAPLGLF 506


Lambda     K      H
   0.320    0.137    0.411 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1005
Number of extensions: 15
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 506
Length of database: 506
Length adjustment: 34
Effective length of query: 472
Effective length of database: 472
Effective search space:   222784
Effective search space used:   222784
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources