GapMind for catabolism of small carbon sources

 

Alignments for a candidate for ech in Klebsiella michiganensis M5al

Align Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17) (characterized)
to candidate BWI76_RS13120 BWI76_RS13120 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase

Query= reanno::psRCH2:GFF2389
         (257 letters)



>FitnessBrowser__Koxy:BWI76_RS13120
          Length = 262

 Score =  137 bits (346), Expect = 2e-37
 Identities = 85/258 (32%), Positives = 140/258 (54%), Gaps = 6/258 (2%)

Query: 6   LLVDIQERVALITLNRPQALNALNGQLISELNQALGQLEADPQIGCIVLTGSAKAFAAGA 65
           +L ++++ V  ITLNRP  LN+ N  +  +L   L Q E D  + C++LTG+ + F AG 
Sbjct: 5   ILSEVEQGVMTITLNRPDRLNSFNDLMHQQLAACLKQAERDDDVRCLLLTGAGRGFCAGQ 64

Query: 66  DIKEM-AELTYPQ----IYLDDFFAD-ADRIATRRKPLIAAVAGYALGGGCELALLCDMI 119
           D+ +   + + P     + ++ F+     R+A   KP+I AV G A G G  LAL CD++
Sbjct: 65  DLNDRNVDPSGPAPDLGLSVERFYNPLVRRLAALPKPVICAVNGVAAGAGATLALGCDIV 124

Query: 120 FAADNARFGQPEVNLGVLPGIGGTQRLTRAVGKAKAMDMCLTGRQMDAAEAERAGLVARV 179
            AA +A+F      LG++P  GG+  L R  G+A+AM + L G  + A +A + G++ +V
Sbjct: 125 LAARSAKFVMAFSKLGLVPDCGGSWFLPRVAGRARAMGLALLGDSLSAEQAAQWGMIWQV 184

Query: 180 FPAESLLEETLKAARVIAEKSLPATMMIKESVNRAFETTLAEGIRFERRVFHAVFATADQ 239
                L +  L  AR +A +      +IK+++  A   TL + +  ER        +AD 
Sbjct: 185 VDDAELKDTGLALARHLAAQPTYGLGLIKKALQLAETQTLDQQLDLERDYQRLAGRSADY 244

Query: 240 KEGMAAFSEKRKPEFTNR 257
           +EG++AF  KR P+F+ +
Sbjct: 245 REGVSAFLAKRPPQFSGK 262


Lambda     K      H
   0.321    0.135    0.375 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 139
Number of extensions: 6
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 257
Length of database: 262
Length adjustment: 24
Effective length of query: 233
Effective length of database: 238
Effective search space:    55454
Effective search space used:    55454
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory