Aligments for a candidate for dctA in Klebsiella michiganensis M5al

GapMind for catabolism of small carbon sources

Aligments for a candidate for dctA in Klebsiella michiganensis M5al

Align C4-dicarboxylate transporter (substrates: fumarate, D- and L-malate, succinate, succinamide, orotate, iticonate, mesaconate) (characterized)
to candidate BWI76_RS08460 BWI76_RS08460 dicarboxylate/amino acid:cation symporter

Query= TCDB::Q01857
         (444 letters)



>FitnessBrowser__Koxy:BWI76_RS08460
          Length = 435

 Score =  275 bits (703), Expect = 2e-78
 Identities = 147/364 (40%), Positives = 226/364 (62%), Gaps = 5/364 (1%)

Query: 54  FIKLVKMIIAPVIFLTVATGIAGMSDLQKVGRVAGKAMLYFLTFSTLALIIGLIVANVVQ 113
           F+++VKMIIAP++  T+  GIA M D + +GR+  K    F+  S L++ +GL++ N+ Q
Sbjct: 47  FLRMVKMIIAPLVISTLVVGIAKMGDAKTLGRIFSKTFFLFICASLLSIALGLVIVNLFQ 106

Query: 114 PGAGMNIDPASLDPAAVATFAAKAHEQSIVGFLTNIIPTTIVGAFADGDILQVLFFSVLF 173
           PGAG+N  P   D  AVA  A ++   ++  F+++ +PT+IV A A  +ILQ++ FS+  
Sbjct: 107 PGAGINFVPH--DAGAVA--AVQSEPFTLKVFISHAVPTSIVDAMARNEILQIVVFSIFL 162

Query: 174 GIALAMVGEKGEQVVNFLNSLTAPVFKLVAILMKAAPIGAFGAMAFTIGKYGVGSIANLA 233
           G +LA +GEK E +V  L+SL   + KL   +M  AP+  F A++  I + G+G + +  
Sbjct: 163 GCSLAAIGEKAEPIVKVLDSLVHVMLKLTGYVMLFAPLTVFAAISGLIAERGLGVMVSAG 222

Query: 234 MLIGTFYITSLLFVFIVLGAVARYNGFSIVALLRYIKEELLLVLGTSSSEAALPGLMNKM 293
           + +G FY+T  +   I++G      G  I  L + I E  LL   TSSSEAA PG ++K+
Sbjct: 223 IFMGEFYLTLGMLWAILIGLSTVIVGPCISRLTKAIFEPALLAFTTSSSEAAFPGTLDKL 282

Query: 294 EKAGCKRSVVGLVIPTGYSFNLDGTNIYMTLAALFIAQATGIHLSWGDQILLLLVAMLSS 353
           EK G    +   V+P GYSFNL G+  Y + A +FIAQA  +HLS G+QI +LL+ ML+S
Sbjct: 283 EKFGVSSKIASFVLPIGYSFNLVGSMAYCSFATVFIAQACNVHLSMGEQITMLLILMLTS 342

Query: 354 KGAAGITGAGFITLAATLSVVPSVPVAGMALILGIDRFMSECRALTNLVGNAVATIVVAR 413
           KG AG+  A  + +AATL+   ++P AG+ L++G+D F+   R+ TN++ NA+   +V R
Sbjct: 343 KGMAGVPRASMVVIAATLNQF-NIPEAGLILLMGVDPFLDMGRSATNVMSNAMGAAIVGR 401

Query: 414 WENE 417
           WE E
Sbjct: 402 WEGE 405



 Score = 25.4 bits (54), Expect = 0.004
 Identities = 48/210 (22%), Positives = 83/210 (39%), Gaps = 51/210 (24%)

Query: 28  VAIAAGILLGHFYPELGTQLKPLGDAFIKLVKMIIAPVIFLTVATGIAGMSDLQKVGRVA 87
           V ++AGI +G FY  LG     L    I L  +I+ P I           S L K   + 
Sbjct: 217 VMVSAGIFMGEFYLTLGM----LWAILIGLSTVIVGPCI-----------SRLTKA--IF 259

Query: 88  GKAMLYFLTFSTLALIIGLIVANVVQPGAGMNIDPASLDPAAVATFAAKAHEQSIVGFLT 147
             A+L F T S+ A            PG    ++   +  + +A+F         +G+  
Sbjct: 260 EPALLAFTTSSSEAAF----------PGTLDKLEKFGVS-SKIASFVLP------IGYSF 302

Query: 148 NIIPTTIVGAFADGDILQVLFFSVLFGIALAMVGEKGEQVVNFLNSLTAPVFKLVAILMK 207
           N++ +    +FA       +F +    + L+M    GEQ+   L      +  L +  M 
Sbjct: 303 NLVGSMAYCSFA------TVFIAQACNVHLSM----GEQITMLL------ILMLTSKGMA 346

Query: 208 AAPIGAFGAMAFTIGKYGVGSIANLAMLIG 237
             P  +   +A T+ ++ +   A L +L+G
Sbjct: 347 GVPRASMVVIAATLNQFNIPE-AGLILLMG 375


Lambda     K      H
   0.324    0.139    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 510
Number of extensions: 27
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 444
Length of database: 435
Length adjustment: 32
Effective length of query: 412
Effective length of database: 403
Effective search space:   166036
Effective search space used:   166036
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources