Alignments for a candidate for odc in Klebsiella michiganensis M5al

GapMind for catabolism of small carbon sources

Alignments for a candidate for odc in Klebsiella michiganensis M5al

Align ornithine decarboxylase (EC 4.1.1.17) (characterized)
to candidate BWI76_RS24175 BWI76_RS24175 ornithine decarboxylase

Query= BRENDA::P21169
         (711 letters)



>FitnessBrowser__Koxy:BWI76_RS24175
          Length = 712

 Score = 1271 bits (3290), Expect = 0.0
 Identities = 609/711 (85%), Positives = 665/711 (93%)

Query: 1   MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSRSGILALLKRTGFHLPV 60
           MKSM+IAAS ELVSRLS+HRRVVAL  TDFTDVAAVVIT ADSRSGILALLKRTGF+LPV
Sbjct: 1   MKSMHIAASRELVSRLSTHRRVVALDSTDFTDVAAVVITVADSRSGILALLKRTGFNLPV 60

Query: 61  FLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPPFYDTLTQYVEMGNSTFAC 120
           +L+SE   E P GV A+++G EQ+WLELE+AAC YEENLLPPF+DTLTQYVEM NSTFAC
Sbjct: 61  YLFSEADHEKPQGVMAIVSGKEQEWLELEAAACGYEENLLPPFFDTLTQYVEMDNSTFAC 120

Query: 121 PGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGDLLIHEGSAKDAQKFAAKVFH 180
           PGHQHGAFFKKHPAGR F+DFFGENVFRADMCNADVKLGDLLIHEGSAK AQKFAAKVF+
Sbjct: 121 PGHQHGAFFKKHPAGRQFFDFFGENVFRADMCNADVKLGDLLIHEGSAKHAQKFAAKVFN 180

Query: 181 ADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKSNHHGALIQAGATPVYLEASRNP 240
           ADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKSNHHGALIQAGATPVYLEASRNP
Sbjct: 181 ADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKSNHHGALIQAGATPVYLEASRNP 240

Query: 241 FGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPYRLAIIQLGTYDGTVYNARQVIDTVG 300
           FGFIGGID  CF+E+YLR  IR+ APEKAD+PRP+RLA+IQLGTYDGTVYNARQV+D +G
Sbjct: 241 FGFIGGIDDRCFDEKYLRDLIRETAPEKADMPRPFRLAVIQLGTYDGTVYNARQVVDKIG 300

Query: 301 HLCDYILFDSAWVGYEQFIPMMADSSPLLLELNENDPGIFVTQSVHKQQAGFSQTSQIHK 360
            LCDYILFDSAWVGYEQFIPMMAD SPLLL+L  +DPGIFVTQSVHKQQAGFSQTSQIHK
Sbjct: 301 SLCDYILFDSAWVGYEQFIPMMADCSPLLLDLTPDDPGIFVTQSVHKQQAGFSQTSQIHK 360

Query: 361 KDNHIRGQARFCPHKRLNNAFMLHASTSPFYPLFAALDVNAKIHEGESGRRLWAECVEIG 420
           KDNH+RGQ RFCPHKRLNNAFMLHASTSPFYPLFAALDVNAKIHEGESGRRLWAECV +G
Sbjct: 361 KDNHLRGQERFCPHKRLNNAFMLHASTSPFYPLFAALDVNAKIHEGESGRRLWAECVALG 420

Query: 421 IEARKAILARCKLFRPFIPPVVDGKLWQDYPTSVLASDRRFFSFEPGAKWHGFEGYAADQ 480
           IEARKAI+A CK+ +PFIPPVV G+ WQD+PT  +A +RRFFSFEPGA+WHGFEGYA+DQ
Sbjct: 421 IEARKAIIANCKMIQPFIPPVVAGRPWQDHPTEAIARERRFFSFEPGARWHGFEGYASDQ 480

Query: 481 YFVDPCKLLLTTPGIDAETGEYSDFGVPATILAHYLRENGIVPEKCDLNSILFLLTPAES 540
           YFVDPCKLLLTTPGIDAE+G+Y+DFG+PATILAHYLRENGIVPEKCDLNSILFLLTPAES
Sbjct: 481 YFVDPCKLLLTTPGIDAESGKYTDFGIPATILAHYLRENGIVPEKCDLNSILFLLTPAES 540

Query: 541 HEKLAQLVAMLAQFEQHIEDDSPLVEVLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDV 600
            EKLAQLVAMLA+FEQHIE D+PL +VLP+++NKYPVRYRDYT+R+LCQEMH+LYVSFDV
Sbjct: 541 AEKLAQLVAMLARFEQHIESDTPLADVLPTIFNKYPVRYRDYTIRELCQEMHNLYVSFDV 600

Query: 601 KDLQKAMFRQQSFPSVVMNPQDAHSAYIRGDVELVRIRDAEGRIAAEGALPYPPGVLCVV 660
           KDLQK MFR++SFP  VMNPQDA+  +IRG+VELVR+  AEGRIAAEGALPYPPGVLCVV
Sbjct: 601 KDLQKEMFRKKSFPRAVMNPQDANREFIRGNVELVRLSAAEGRIAAEGALPYPPGVLCVV 660

Query: 661 PGEVWGGAVQRYFLALEEGVNLLPGFSPELQGVYSETDADGVKRLYGYVLK 711
           PGE+WGGAV RYFLALEEGVN+LPGFSPELQGVYSETD DG+KRLYGYVLK
Sbjct: 661 PGEIWGGAVLRYFLALEEGVNMLPGFSPELQGVYSETDPDGIKRLYGYVLK 711


Lambda     K      H
   0.322    0.138    0.420 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1684
Number of extensions: 53
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 711
Length of database: 712
Length adjustment: 39
Effective length of query: 672
Effective length of database: 673
Effective search space:   452256
Effective search space used:   452256
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources