Align putrescine-2-oxoglutarate transaminase (EC 2.6.1.82) (characterized)
to candidate BWI76_RS24630 BWI76_RS24630 putrescine aminotransferase
Query= BRENDA::P42588 (459 letters) >FitnessBrowser__Koxy:BWI76_RS24630 Length = 468 Score = 865 bits (2235), Expect = 0.0 Identities = 433/459 (94%), Positives = 447/459 (97%) Query: 1 MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKEHVNPGFLEYRKSVTAG 60 +NRLPSSASALACSAHALNLIEKRTLDHEEMK+LN+EV EYFKEHVNPGFL YRKSVTAG Sbjct: 10 LNRLPSSASALACSAHALNLIEKRTLDHEEMKSLNQEVREYFKEHVNPGFLAYRKSVTAG 69 Query: 61 GDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPVVVSAVQNQLAKQPLHSQE 120 GDYGAVEWQAG LNTLVDTQGQEFIDCLGGFGIFNVGHRNPVVVSAV+NQLAKQPLHSQE Sbjct: 70 GDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPVVVSAVENQLAKQPLHSQE 129 Query: 121 LLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKLAKAYQSPRGKFTFIATSGAF 180 LLDPLRAMLAKTLAALTPGKLKYSFF NSGTESVEAALKLAKAYQSPRGKFTF+ATSGAF Sbjct: 130 LLDPLRAMLAKTLAALTPGKLKYSFFSNSGTESVEAALKLAKAYQSPRGKFTFVATSGAF 189 Query: 181 HGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAMRTALNECKKTGDDVAAVILEPI 240 HGKSLGALSATAKSTFRKPFMPLLPGFRHVPFG+I AMRT L+ECKKTGDDVAAVILEPI Sbjct: 190 HGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGDINAMRTLLSECKKTGDDVAAVILEPI 249 Query: 241 QGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTGMGRTGKMFACEHENVQPDILCLAK 300 QGEGGVILPPPGYL AVRKLCDEFGAL+ILDEVQTGMGRTGKMFACEHENVQPDILCLAK Sbjct: 250 QGEGGVILPPPGYLPAVRKLCDEFGALLILDEVQTGMGRTGKMFACEHENVQPDILCLAK 309 Query: 301 ALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFGGNPLACAAALATINVLLEQNLPAQAE 360 ALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFGGNPLACAAALATINVLL QNLPAQAE Sbjct: 310 ALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFGGNPLACAAALATINVLLTQNLPAQAE 369 Query: 361 QKGDMLLDGFRQLAREYPDLVQEARGKGMLMAIEFVDNEIGYNFASEMFRQRVLVAGTLN 420 QKGDMLLDGFRQLAREYPDLV E RGKGMLMAIEFVDNEIGY+FASEMFRQRVLVAGTLN Sbjct: 370 QKGDMLLDGFRQLAREYPDLVNEVRGKGMLMAIEFVDNEIGYDFASEMFRQRVLVAGTLN 429 Query: 421 NAKTIRIEPPLTLTIEQCELVIKAARKALAAMRVSVEEA 459 NA+TIR+EPPLTLT+EQCE V+KAAR ALAA+RVSVEEA Sbjct: 430 NARTIRVEPPLTLTLEQCEQVLKAARLALAALRVSVEEA 468 Lambda K H 0.320 0.135 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 848 Number of extensions: 24 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 459 Length of database: 468 Length adjustment: 33 Effective length of query: 426 Effective length of database: 435 Effective search space: 185310 Effective search space used: 185310 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 51 (24.3 bits)
Align candidate BWI76_RS24630 BWI76_RS24630 (putrescine aminotransferase)
to HMM TIGR03372 (ygjG: putrescine aminotransferase (EC 2.6.1.82))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR03372.hmm # target sequence database: /tmp/gapView.11419.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR03372 [M=442] Accession: TIGR03372 Description: putres_am_tran: putrescine aminotransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3e-291 951.5 0.5 3.4e-291 951.3 0.5 1.0 1 lcl|FitnessBrowser__Koxy:BWI76_RS24630 BWI76_RS24630 putrescine aminotr Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Koxy:BWI76_RS24630 BWI76_RS24630 putrescine aminotransferase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 951.3 0.5 3.4e-291 3.4e-291 1 442 [] 17 458 .. 17 458 .. 1.00 Alignments for each domain: == domain 1 score: 951.3 bits; conditional E-value: 3.4e-291 TIGR03372 1 lsalecsaqalnliekdtldheemkalnrevleyfkeyvnpgfleyrksvtaggdygavewrasglntlvd 71 +sal+csa+alnliek+tldheemk+ln+ev eyfke+vnpgfl yrksvtaggdygavew+a+glntlvd lcl|FitnessBrowser__Koxy:BWI76_RS24630 17 ASALACSAHALNLIEKRTLDHEEMKSLNQEVREYFKEHVNPGFLAYRKSVTAGGDYGAVEWQAGGLNTLVD 87 589******************************************************************** PP TIGR03372 72 tqgqeyldclggygifnvghrnpvvvaavesqlakqplhsqelldplrallaktlaaltpgklkysffsns 142 tqgqe++dclgg+gifnvghrnpvvv+ave+qlakqplhsqelldplra+laktlaaltpgklkysffsns lcl|FitnessBrowser__Koxy:BWI76_RS24630 88 TQGQEFIDCLGGFGIFNVGHRNPVVVSAVENQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFSNS 158 *********************************************************************** PP TIGR03372 143 gtesveaalklakayqsprgkytfiaasgafhgkslgalsataksafrkpfmpllpgfrhvafgdieamlk 213 gtesveaalklakayqsprgk+tf+a+sgafhgkslgalsataks+frkpfmpllpgfrhv+fgdi+am++ lcl|FitnessBrowser__Koxy:BWI76_RS24630 159 GTESVEAALKLAKAYQSPRGKFTFVATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGDINAMRT 229 *********************************************************************** PP TIGR03372 214 alqeckktgddvaavilepiqgeggvilppenyltavrklcdevgallildevqtgmgrtgkmfacehenv 284 l eckktgddvaavilepiqgeggvilpp++yl+avrklcde+gallildevqtgmgrtgkmfacehenv lcl|FitnessBrowser__Koxy:BWI76_RS24630 230 LLSECKKTGDDVAAVILEPIQGEGGVILPPPGYLPAVRKLCDEFGALLILDEVQTGMGRTGKMFACEHENV 300 *********************************************************************** PP TIGR03372 285 qpdilclakalgggvmpigatvateevfsvlfenpflhtttfggnplacaaalatvnvlleknlpeqaeik 355 qpdilclakalgggvmpigat+ateevfsvlf+npflhtttfggnplacaaalat+nvll++nlp+qae+k lcl|FitnessBrowser__Koxy:BWI76_RS24630 301 QPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFGGNPLACAAALATINVLLTQNLPAQAEQK 371 *********************************************************************** PP TIGR03372 356 gellldglrqlaaeypdlvieargkgllqaiefvdneigyafakelfrrrvlvagtlnnaksvrieppltl 426 g++lldg+rqla+eypdlv e rgkg+l+aiefvdneigy+fa+e+fr+rvlvagtlnna+++r+eppltl lcl|FitnessBrowser__Koxy:BWI76_RS24630 372 GDMLLDGFRQLAREYPDLVNEVRGKGMLMAIEFVDNEIGYDFASEMFRQRVLVAGTLNNARTIRVEPPLTL 442 *********************************************************************** PP TIGR03372 427 tieqcelvlkeakdvl 442 t eqce+vlk+a+ +l lcl|FitnessBrowser__Koxy:BWI76_RS24630 443 TLEQCEQVLKAARLAL 458 ************9765 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (442 nodes) Target sequences: 1 (468 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.01 # Mc/sec: 12.64 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory