GapMind for catabolism of small carbon sources

 

Alignments for a candidate for puuA in Klebsiella michiganensis M5al

Align Glutamate--putrescine ligase (EC 6.3.1.11) (characterized)
to candidate BWI76_RS10710 BWI76_RS10710 gamma-glutamylputrescine synthetase

Query= reanno::Koxy:BWI76_RS10710
         (472 letters)



>FitnessBrowser__Koxy:BWI76_RS10710
          Length = 472

 Score =  964 bits (2491), Expect = 0.0
 Identities = 472/472 (100%), Positives = 472/472 (100%)

Query: 1   METNIVEVENFVQQSEERRVSAFTWEVKRYLERYPNTQYVDVLLTDLNGCFRGKRIPVSG 60
           METNIVEVENFVQQSEERRVSAFTWEVKRYLERYPNTQYVDVLLTDLNGCFRGKRIPVSG
Sbjct: 1   METNIVEVENFVQQSEERRVSAFTWEVKRYLERYPNTQYVDVLLTDLNGCFRGKRIPVSG 60

Query: 61  LSKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVPVLGTLTPSAADPEYIGQV 120
           LSKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVPVLGTLTPSAADPEYIGQV
Sbjct: 61  LSKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVPVLGTLTPSAADPEYIGQV 120

Query: 121 LLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVELEFYLLDRKRDAEGYLQPPCA 180
           LLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVELEFYLLDRKRDAEGYLQPPCA
Sbjct: 121 LLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVELEFYLLDRKRDAEGYLQPPCA 180

Query: 181 PGTGDRNTQSQVYSVDNLNHFADVLNEIDEIAQLQLIPADGAVAEASPGQFEINLHHTDN 240
           PGTGDRNTQSQVYSVDNLNHFADVLNEIDEIAQLQLIPADGAVAEASPGQFEINLHHTDN
Sbjct: 181 PGTGDRNTQSQVYSVDNLNHFADVLNEIDEIAQLQLIPADGAVAEASPGQFEINLHHTDN 240

Query: 241 VLDACDDALALKRLVRLMAEKHKMHATFMAKPYEEHAGSGMHIHISMQNNKGENVLADAD 300
           VLDACDDALALKRLVRLMAEKHKMHATFMAKPYEEHAGSGMHIHISMQNNKGENVLADAD
Sbjct: 241 VLDACDDALALKRLVRLMAEKHKMHATFMAKPYEEHAGSGMHIHISMQNNKGENVLADAD 300

Query: 301 GEDSAMLKRALAGMIDLMPASMALLAPNVNSYRRFQPGMYVPTQASWGHNNRTVALRIPC 360
           GEDSAMLKRALAGMIDLMPASMALLAPNVNSYRRFQPGMYVPTQASWGHNNRTVALRIPC
Sbjct: 301 GEDSAMLKRALAGMIDLMPASMALLAPNVNSYRRFQPGMYVPTQASWGHNNRTVALRIPC 360

Query: 361 GDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNPLPLQEEVEGNGLEQEGLPFPIRQ 420
           GDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNPLPLQEEVEGNGLEQEGLPFPIRQ
Sbjct: 361 GDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNPLPLQEEVEGNGLEQEGLPFPIRQ 420

Query: 421 SDALWEFMQNDHLRERLGERFCHVYHACKNDELLQFERLITETEIEWMLKNA 472
           SDALWEFMQNDHLRERLGERFCHVYHACKNDELLQFERLITETEIEWMLKNA
Sbjct: 421 SDALWEFMQNDHLRERLGERFCHVYHACKNDELLQFERLITETEIEWMLKNA 472


Lambda     K      H
   0.319    0.136    0.411 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 956
Number of extensions: 21
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 472
Length of database: 472
Length adjustment: 33
Effective length of query: 439
Effective length of database: 439
Effective search space:   192721
Effective search space used:   192721
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory