Align Glutamate/aspartate import permease protein GltJ (characterized)
to candidate BWI76_RS08105 BWI76_RS08105 glutamate/aspartate ABC transporter permease GltJ
Query= SwissProt::P0AER3 (246 letters) >FitnessBrowser__Koxy:BWI76_RS08105 Length = 246 Score = 456 bits (1172), Expect = e-133 Identities = 221/246 (89%), Positives = 231/246 (93%) Query: 1 MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAFLVGSFFGILRTVPNRF 60 MSIDWNWGIFLQQAPFGNTTYLGW+WSGFQVT+ LS+ AWIIAF VGS FGILRTVPNR Sbjct: 1 MSIDWNWGIFLQQAPFGNTTYLGWLWSGFQVTVTLSVTAWIIAFFVGSLFGILRTVPNRL 60 Query: 61 LSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKAELDPNIQFFLSSMLCLGL 120 LS +GT YVELFRNVPLIVQFFTWYLV+PELLPE IGMWFK+ELDPNIQFF+SSMLCLGL Sbjct: 61 LSTIGTCYVELFRNVPLIVQFFTWYLVVPELLPENIGMWFKSELDPNIQFFVSSMLCLGL 120 Query: 121 FTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVLLPNAYRVIVPPMTSEMMNLV 180 FTAARVCEQVR+ IQSLPRGQKNA LAMGLTLPQ YRYVLLPNAYRVIVPPMTSEMMNLV Sbjct: 121 FTAARVCEQVRSGIQSLPRGQKNAGLAMGLTLPQTYRYVLLPNAYRVIVPPMTSEMMNLV 180 Query: 181 KNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLAYVLINAFIMLVMTLVERKVRLP 240 KNSAIASTIGL DMAAQAGKLLDYSAHAWESFTAITLAYV INA IML+M +VERKVRLP Sbjct: 181 KNSAIASTIGLADMAAQAGKLLDYSAHAWESFTAITLAYVFINAVIMLIMYVVERKVRLP 240 Query: 241 GNMGGK 246 GNMGGK Sbjct: 241 GNMGGK 246 Lambda K H 0.328 0.140 0.443 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 349 Number of extensions: 6 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 246 Length of database: 246 Length adjustment: 24 Effective length of query: 222 Effective length of database: 222 Effective search space: 49284 Effective search space used: 49284 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory