Aligments for a candidate for glt in Klebsiella michiganensis M5al

GapMind for catabolism of small carbon sources

Aligments for a candidate for glt in Klebsiella michiganensis M5al

Align Amino acid:proton symporter (characterized, see rationale)
to candidate BWI76_RS08460 BWI76_RS08460 dicarboxylate/amino acid:cation symporter

Query= uniprot:A0A0N9WTL5
         (431 letters)



>lcl|FitnessBrowser__Koxy:BWI76_RS08460 BWI76_RS08460
           dicarboxylate/amino acid:cation symporter
          Length = 435

 Score =  393 bits (1009), Expect = e-114
 Identities = 215/433 (49%), Positives = 297/433 (68%), Gaps = 8/433 (1%)

Query: 1   MKKNKLPRRIAMGIALGVLVGWACHHFAGSEQSAKEIASYFSMVTDIFLRMIKMIIAPLV 60
           MKK  L + I +G+ LG++ G   ++ A ++ +AK  A   S+ T IFLRM+KMIIAPLV
Sbjct: 1   MKKISLTKMIILGLILGMIAGVIINNMASAD-TAKSYAQDISIFTTIFLRMVKMIIAPLV 59

Query: 61  FATLVGGIASMGNSRSVGRIGARAMAWFVTASVVSLLIGMGLVNLFQPGAGLNMDVAQHA 120
            +TLV GIA MG+++++GRI ++    F+ AS++S+ +G+ +VNLFQPGAG+N  V   A
Sbjct: 60  ISTLVVGIAKMGDAKTLGRIFSKTFFLFICASLLSIALGLVIVNLFQPGAGINF-VPHDA 118

Query: 121 TAAVPVNTGDFSLKAFIGHVFPRSIAEAMANNEILQIVVFSLFFGFALA--GVKRAGYTR 178
            A   V +  F+LK FI H  P SI +AMA NEILQIVVFS+F G +LA  G K     +
Sbjct: 119 GAVAAVQSEPFTLKVFISHAVPTSIVDAMARNEILQIVVFSIFLGCSLAAIGEKAEPIVK 178

Query: 179 ITDSIEELAKVMFKITDYVMAFAPIGVFAAIASAITTQGLGLLVDYGKLIAEFYLGILIL 238
           + DS   L  VM K+T YVM FAP+ VFAAI+  I  +GLG++V  G  + EFYL + +L
Sbjct: 179 VLDS---LVHVMLKLTGYVMLFAPLTVFAAISGLIAERGLGVMVSAGIFMGEFYLTLGML 235

Query: 239 WALLFGAGYLFLGRSVFHLGKLIREPILLAFSTASSESAYPKTIEALEKFGAPKRVSSFV 298
           WA+L G   + +G  +  L K I EP LLAF+T+SSE+A+P T++ LEKFG   +++SFV
Sbjct: 236 WAILIGLSTVIVGPCISRLTKAIFEPALLAFTTSSSEAAFPGTLDKLEKFGVSSKIASFV 295

Query: 299 LPLGYSFNLDGSMMYQAFAILFIAQAYNIDLSFTQQLLILLTLMITSKGMAGVARASVVV 358
           LP+GYSFNL GSM Y +FA +FIAQA N+ LS  +Q+ +LL LM+TSKGMAGV RAS+VV
Sbjct: 296 LPIGYSFNLVGSMAYCSFATVFIAQACNVHLSMGEQITMLLILMLTSKGMAGVPRASMVV 355

Query: 359 VAATLPMFNLPEAGLLLIIGIDQFLDMARTATNVVGNSIATAVVAKSESHEEADEEEGEH 418
           +AATL  FN+PEAGL+L++G+D FLDM R+ATNV+ N++  A+V + E     +   G  
Sbjct: 356 IAATLNQFNIPEAGLILLMGVDPFLDMGRSATNVMSNAMGAAIVGRWEGEHYGEGCRGT- 414

Query: 419 APARSRSEPVPVA 431
           AP +   +  P +
Sbjct: 415 APVKKPEQDRPAS 427


Lambda     K      H
   0.325    0.138    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 460
Number of extensions: 20
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 431
Length of database: 435
Length adjustment: 32
Effective length of query: 399
Effective length of database: 403
Effective search space:   160797
Effective search space used:   160797
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources