Align TM0030, component of β-glucoside porter (Conners et al., 2005). Binds cellobiose, laminaribiose (Nanavati et al. 2006). Regulated by cellobiose-responsive repressor BglR (characterized)
to candidate BWI76_RS07525 BWI76_RS07525 ABC transporter permease
Query= TCDB::Q9WXN7 (338 letters) >FitnessBrowser__Koxy:BWI76_RS07525 Length = 317 Score = 180 bits (456), Expect = 5e-50 Identities = 105/328 (32%), Positives = 175/328 (53%), Gaps = 13/328 (3%) Query: 6 MFKYLLRRFIFLLVTYIVATTIVFILPRAIPGNPLSQILSGLSRVAQANPEAIRAAERTL 65 M LL R + + T + I F+L + PG+PL ++ P+A + +TL Sbjct: 1 MINTLLYRILLAVPTMLGVAVICFMLVQIAPGDPLVSVMP---------PDASESLRQTL 51 Query: 66 MEEFGLGKPWYVQYFEFITKALRGDLGTSITFYPRKVIDLIIPVIPWTLILLLPATIVAW 125 M+ +G KP +Q+ ++ +AL GDLG S+ R VID ++ + ++L L L AT + + Sbjct: 52 MQAYGFDKPLPLQFVHWLWRALHGDLGMSVAT-GRPVIDEVMTAVTYSLRLALLATAIGF 110 Query: 126 ILGNSLGALAAYKRNTWIDKGVLTTSLIVSQIPYYWLGMIFIFLFGVKLGWLPVQGAYSQ 185 +LG+ G +A Y RN+ ID+ S+ +P+YWLGM+ + +F VK LP G Sbjct: 111 VLGSLFGFVAGYFRNSIIDRLASVLSVFGVSVPHYWLGMLLVIVFSVKFALLPATGG--- 167 Query: 186 GTIPNLSWSFFVDVLKHYIMPFASIVVSAMGGWAIGMRLMVIYELGSDYAMFSEYLGMKD 245 G I + W + + L+ ++P ++ V G A +R V L ++ + G+ + Sbjct: 168 GPIGEVGWQWDWEHLQFMLLPAFTLSVIPTGIIARTVRSQVADILSQEFIVGLRARGLNE 227 Query: 246 KRIFKYVFRNSLLPQITGLALSLGGVLGGALITEIVFNYPGTGYLLFRALTTLDYPLIQG 305 RIF +V +N+ + + L +G ++GG+++ E VF++PGTG LL A+ D PL+QG Sbjct: 228 SRIFLHVVKNAAPTALAVMGLQVGYLMGGSILVETVFSWPGTGLLLNTAIFQRDLPLLQG 287 Query: 306 IFVILIASIYLANFIVDFLYALIDPRIR 333 +L L N +VD L +DPRI+ Sbjct: 288 TIWVLALFFVLLNLLVDILQTSLDPRIK 315 Lambda K H 0.329 0.146 0.449 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 300 Number of extensions: 20 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 338 Length of database: 317 Length adjustment: 28 Effective length of query: 310 Effective length of database: 289 Effective search space: 89590 Effective search space used: 89590 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 48 (23.1 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory