Align 6-P-β-glucosidase (ChbF;ECs2440) (EC 3.2.1.86) (characterized)
to candidate BWI76_RS11745 BWI76_RS11745 6-phospho-beta-glucosidase
Query= CAZy::BAB35863.1 (450 letters) >FitnessBrowser__Koxy:BWI76_RS11745 Length = 448 Score = 793 bits (2049), Expect = 0.0 Identities = 386/448 (86%), Positives = 419/448 (93%) Query: 1 MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGGKAKLDIIFDLCQRMID 60 MSQKLKVVTIGGGSSYTPELLEGF+KRYHELP+SELWLVDV G+ KLDII LCQRM++ Sbjct: 1 MSQKLKVVTIGGGSSYTPELLEGFLKRYHELPISELWLVDVAEGQEKLDIIHALCQRMVE 60 Query: 61 NAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERIPLSHGYLGQETNGAGGLF 120 AGVPMK+YKTLDRR AL+ ADFVTTQLRVGQL ARE DERIPLSHGYLGQETNGAGGLF Sbjct: 61 KAGVPMKVYKTLDRRAALEGADFVTTQLRVGQLKAREKDERIPLSHGYLGQETNGAGGLF 120 Query: 121 KGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVYRHTGFKRFIGVCNIPIGMKM 180 KGLRTIPVIFDIVKDV+E+CPNAWVINFTNPAGMVTEAVYRHTGFKRFIGVCNIPIGMKM Sbjct: 121 KGLRTIPVIFDIVKDVQEVCPNAWVINFTNPAGMVTEAVYRHTGFKRFIGVCNIPIGMKM 180 Query: 181 FIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRFAELLDGVASGQLKASGVKNIFD 240 FI DVL L SD+L+IDLFGLNH+VF++DVLVNG SRF ELLDGVASG+L A+ VKNIFD Sbjct: 181 FITDVLKLAPSDELNIDLFGLNHLVFVRDVLVNGVSRFDELLDGVASGRLSANSVKNIFD 240 Query: 241 LPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEYYKGGARAQVVQKVEKQLFELYKNP 300 LPFSEGL+RSL L+PCSYLLYYFK KEMLAIEMGEYYKGGARAQVVQKVEKQLFELYK+P Sbjct: 241 LPFSEGLLRSLRLIPCSYLLYYFKPKEMLAIEMGEYYKGGARAQVVQKVEKQLFELYKDP 300 Query: 301 ELKVKPKELEQRGGAYYSDAACEVINAIYNDKQAEHYVNIPHHGHIDNIPADWAVEMTCT 360 +L VKPKELEQRGGAYYSDAACEVINAIYNDKQAEHYVN+PHHGH+DNIPADWAVEMTCT Sbjct: 301 DLNVKPKELEQRGGAYYSDAACEVINAIYNDKQAEHYVNVPHHGHVDNIPADWAVEMTCT 360 Query: 361 LGRDGATPHPRITHFDDKVMGLIHTIKGFEIAASNAALSGEFNDVLLALNLSPLVHSDRD 420 LGRDGA P PRITHFD+KV+GLI+TIKGFE+AAS AA+SG+ +DVLLALNLSPL+HSDRD Sbjct: 361 LGRDGAKPTPRITHFDEKVLGLIYTIKGFEVAASEAAISGKLDDVLLALNLSPLIHSDRD 420 Query: 421 AELLAREMILAHEKWLPNFADCIAELKK 448 AE LAREMILAHE+WLPNFA I +LK+ Sbjct: 421 AEKLAREMILAHEQWLPNFAATIEKLKQ 448 Lambda K H 0.321 0.140 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 837 Number of extensions: 22 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 450 Length of database: 448 Length adjustment: 33 Effective length of query: 417 Effective length of database: 415 Effective search space: 173055 Effective search space used: 173055 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 51 (24.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory