Align PTS beta-glucoside transporter, EIIBCA of 672 aas and 12 predicted TMSs (characterized)
to candidate BWI76_RS07290 BWI76_RS07290 PTS beta-glucoside transporter subunit EIIBCA
Query= TCDB::ART98499 (672 letters) >FitnessBrowser__Koxy:BWI76_RS07290 Length = 626 Score = 390 bits (1002), Expect = e-112 Identities = 222/666 (33%), Positives = 362/666 (54%), Gaps = 46/666 (6%) Query: 4 NYDALAKTIIKDVGGKDNVISVVHCTTRLRFKLKDEKKANDDALKDTDGVVTVVKAGGQY 63 NY+ A I+ VGG +N+ + HC TRLR + D K ND + GV++VV+ GGQ+ Sbjct: 2 NYNETADKILHAVGGAENIRMLTHCATRLRMEFNDRAKVNDAQIGTLPGVISVVEKGGQF 61 Query: 64 QVVIGNEVADVY----EAVLKEGGFSGGGQVADDDLDDSNMSLMDKAIDLISGIFTPILG 119 Q+VIGNEV VY +A+ ++ G +GG D + ++ + I +IS FTP++ Sbjct: 62 QIVIGNEVQQVYRRLNKALPEKKGPAGG------DKNAKPAGIVARIISVISTTFTPVIP 115 Query: 120 PMAAAGMIKGLTAMCASFGWLAKTSGTYEVLYAIGDGFFYFLPLILAITSAKKFKVDRFT 179 + AGMIK L A+ G +++TS TY++L I D F+FLP++LA ++ KF+ + Sbjct: 116 AITGAGMIKALLAILKLTGVISETSSTYQLLNIIADAAFFFLPVLLAYGASLKFECNPIL 175 Query: 180 AITIGAAMCYPAMVAMNSSKKVLFDLFNGTFLHSQVHATFLGIPIISMNYTSTVIPIILA 239 A+T+ + +P + M ++ K + F+GI ++ +Y +V+PIIL Sbjct: 176 AMTLAGVLLHPGIGQMLAAGKAV---------------DFVGINVLLSDYAGSVLPIILT 220 Query: 240 VWFASVVEKWCKEWIPTVVKTFLVPFVTLLIVVPLTFLIIGPLATWIGNALAAITSAVYN 299 VW S VE++ ++ P+++K F+ P + LLI PL +++GP + + +AA A+ Sbjct: 221 VWLMSWVERFAEKVSPSIIKFFVKPMLILLITAPLALVVVGPAGILLNDLVAAGAGAIDR 280 Query: 300 FSPVLAGILLGGFWQVFVIFGVHWGFVAVMMSNIAALGYDPILGLSLGASFAQIGVV-LA 358 + L +L+G +I G W + ++ GY+ I G + AS +G L Sbjct: 281 HASWLIPMLMGTLQPFLIITGTAWAMTPIATGQLSKSGYEMINGPGMLASNVAMGAATLC 340 Query: 359 ILLQTKDQKLKGIALPAFLSGIFGVTEPAIYGVTLPRKKPFILSCIAAGVGGGLIGFFGT 418 + L+TK+ L+ +A + + + G+TEPA+YGV L ++ I + I G G G G Sbjct: 341 VALKTKNSNLRQLASSSGFTALLGITEPALYGVLLKFRRVLIAAMIGGGCAGVYAGVSGL 400 Query: 419 KMYMMGGMGVFVIPAAIGPKTGVDMSVYGLMIAMAVSFVLGFILQMALGKKSVDQAYDEK 478 Y G+ +PA IG M++ ++ A+S V+ F L + K V + +E Sbjct: 401 VRYAFVSPGLAALPAFIGENP---MNIVHALVTCAISIVVTFTLTWFIAFKDVPEEQEE- 456 Query: 479 QAKAVQEVASQADAIAKAAPSLASTSDLNVSTELVSPLAGELLPLSEVKDEVFSSGAMGE 538 AP A + TE+ SPL G+++ LSEV D+VFS G +GE Sbjct: 457 ---------------VGEAPQPAVSPKNEGETEVFSPLRGQVVALSEVNDDVFSGGLLGE 501 Query: 539 GVAVEPSEGVLHAPADGKVVMTFPTGHAIGMKTSDGAEILMHIGMDTVNLQGHGFETLVA 598 G+A+ P EGVL AP G V+M P+ HA+G+++ G E+L+HIG+DTVNL G F + + Sbjct: 502 GLAIRPEEGVLRAPFSGTVMMFLPSCHAVGLQSDSGLELLIHIGIDTVNLNGQHFSSTLK 561 Query: 599 KGDEVKAGDELVKFDIDAIHAKGYVVTTPIVVTNSKDYEKITVVSQGKVKVGQEILDLEG 658 GD V+AG EL++FDI AI GY + TP++V N + + + + +V G++++ ++ Sbjct: 562 VGDRVEAGQELIRFDIPAIEQAGYDLITPVIVVNGDEQHSLRLTAAAQVDYGEQLM-VQS 620 Query: 659 ASETEA 664 A E +A Sbjct: 621 AKEAQA 626 Lambda K H 0.321 0.137 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1014 Number of extensions: 49 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 672 Length of database: 626 Length adjustment: 38 Effective length of query: 634 Effective length of database: 588 Effective search space: 372792 Effective search space used: 372792 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory