Aligments for a candidate for citF in Klebsiella michiganensis M5al

GapMind for catabolism of small carbon sources

Aligments for a candidate for citF in Klebsiella michiganensis M5al

Align Citrate lyase alpha chain; Citrase alpha chain; Citrate (pro-3S)-lyase alpha chain; Citrate CoA-transferase subunit; EC 4.1.3.6; EC 2.8.3.10 (characterized)
to candidate BWI76_RS04450 BWI76_RS04450 citrate lyase subunit alpha

Query= SwissProt::P75726
         (510 letters)



>lcl|FitnessBrowser__Koxy:BWI76_RS04450 BWI76_RS04450 citrate lyase
           subunit alpha
          Length = 508

 Score =  697 bits (1800), Expect = 0.0
 Identities = 355/479 (74%), Positives = 413/479 (86%), Gaps = 1/479 (0%)

Query: 31  SPKQTYQAEKARDRKLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGF 90
           SP    ++EK R RK+CA+LEEAIRRSGLQ+GMT+SFHHAFRGGD  VNMV+  +A+MGF
Sbjct: 28  SPWLASESEK-RQRKICASLEEAIRRSGLQNGMTISFHHAFRGGDKVVNMVVAKLAEMGF 86

Query: 91  KNLTLASSSLSDCHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRV 150
           ++LTLASSSL D H PL+EHI+ GV+ +IYTSGLRG L EEIS GL+  PVQIHSHGGR 
Sbjct: 87  RDLTLASSSLIDAHWPLIEHIKNGVIRQIYTSGLRGKLGEEISAGLMENPVQIHSHGGRA 146

Query: 151 HLVQSGELNIDVAFLGVPSCDEFGNANGYTGKACCGSLGYAIVDADNAKQVVMLTEELLP 210
           +LVQSGELNIDVAFLGVP CDE+GNANG++GK+ CGSLGYA VDAD AK VV+LTEE + 
Sbjct: 147 YLVQSGELNIDVAFLGVPCCDEYGNANGFSGKSRCGSLGYAKVDADAAKCVVLLTEEWVD 206

Query: 211 YPHNPASIEQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKE 270
           YP+ PASI QDQVDLIV+VD VGD AKI AGA R+T+NPRELLIAR AA VI +SGYFK+
Sbjct: 207 YPNYPASIAQDQVDLIVQVDEVGDPAKITAGAIRLTSNPRELLIARQAAKVIEHSGYFKD 266

Query: 271 GFSMQTGTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFD 330
           GFS+QTGTGGASLAVTRFLEDKMR  +I A F LGGIT TMVDLHEKGLI+ LLD QSFD
Sbjct: 267 GFSLQTGTGGASLAVTRFLEDKMRRHNITASFGLGGITGTMVDLHEKGLIKALLDTQSFD 326

Query: 331 SHAAQSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVL 390
             AA+SLA NPNHIEISANQYAN GSKG + +RL+VV+LSALEIDT FNVNV+TGS+GVL
Sbjct: 327 GDAARSLANNPNHIEISANQYANPGSKGIACERLNVVMLSALEIDTGFNVNVMTGSNGVL 386

Query: 391 RGASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR 450
           RGASGGHCDTA  + L+II APLVRGRIP +V+ VLT +TPG+SVD+LVTDHGIAVNPAR
Sbjct: 387 RGASGGHCDTAAGADLTIITAPLVRGRIPCVVEKVLTRVTPGASVDVLVTDHGIAVNPAR 446

Query: 451 PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQVK 509
            +L +RL++A I +++IE L++RA LLTG+P+PI FTDRVVAVVRYRDGSVIDV+HQVK
Sbjct: 447 QDLIDRLRQADIPLMTIEALQQRAELLTGKPEPIAFTDRVVAVVRYRDGSVIDVIHQVK 505


Lambda     K      H
   0.319    0.134    0.384 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 826
Number of extensions: 25
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 510
Length of database: 508
Length adjustment: 34
Effective length of query: 476
Effective length of database: 474
Effective search space:   225624
Effective search space used:   225624
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources