GapMind for catabolism of small carbon sources

 

Aligments for a candidate for PS417_17600 in Klebsiella michiganensis M5al

Align ABC transporter permease; SubName: Full=Amino acid ABC transporter permease; SubName: Full=Histidine ABC transporter permease HisM; SubName: Full=Histidine transport system permease protein; SubName: Full=Histidine/lysine/arginine/ornithine ABC transporter permease HisM (characterized, see rationale)
to candidate BWI76_RS20325 BWI76_RS20325 histidine/lysine/arginine/ornithine ABC transporter permease HisM

Query= uniprot:A0A1N7U128
         (237 letters)



>lcl|FitnessBrowser__Koxy:BWI76_RS20325 BWI76_RS20325
           histidine/lysine/arginine/ornithine ABC transporter
           permease HisM
          Length = 238

 Score =  287 bits (734), Expect = 1e-82
 Identities = 135/235 (57%), Positives = 190/235 (80%)

Query: 1   MIELFQQYGLAYLFSDGAGLSGVAMTLWLFIISVVLGFFLSIPLALARVSEHVWLRWPVE 60
           MI++ Q+Y  + L++DG   +GVA+TLWL I SVV+G  L++ LA+ RVS + ++++P+ 
Sbjct: 1   MIDIIQEYWKSLLWTDGYRFTGVAITLWLLISSVVMGGILAVFLAIGRVSSNKFIQFPIW 60

Query: 61  VYTYLFRGTPLYIQLLICYTGLYSLEIVQDNALLNQFFRNALNCTLLAFVLNTCAYTVEI 120
           ++TY+FRGTPLY+QLL+ Y+G+Y+LEIV+   LLN FFR+ LNCT+LA  LNTCAYT EI
Sbjct: 61  LFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTELLNAFFRSGLNCTVLALTLNTCAYTTEI 120

Query: 121 FAGAIRNIPHGEIEAARAYGLHGWRLNLFVVVPAALRRALPAYSNEMILMLHATSLAFTA 180
           FAGAIR++P GEIEAARAYG    ++   +++P+ALR ALPAYSNE+ILMLH+T+LAFTA
Sbjct: 121 FAGAIRSVPAGEIEAARAYGFSSVKMYRCIILPSALRIALPAYSNEVILMLHSTALAFTA 180

Query: 181 TVADILKVARDANAETFLTFQAFGIAALLYMLLSFALVGLFRLAERRWMRFLVPT 235
           TV D+LK+ARD N+ T+  F AFGIAA+LY+++S+ L+ LFR AE+RW++ + P+
Sbjct: 181 TVPDLLKIARDINSATYQPFTAFGIAAVLYLIISYVLISLFRRAEKRWLQHIKPS 235


Lambda     K      H
   0.332    0.143    0.443 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 235
Number of extensions: 10
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 237
Length of database: 238
Length adjustment: 23
Effective length of query: 214
Effective length of database: 215
Effective search space:    46010
Effective search space used:    46010
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (22.0 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory