Alignments for a candidate for fruI in Klebsiella michiganensis M5al

GapMind for catabolism of small carbon sources

Alignments for a candidate for fruI in Klebsiella michiganensis M5al

Align Phosphoenolpyruvate--protein phosphotransferase (EC 2.7.3.9) (characterized)
to candidate BWI76_RS20650 BWI76_RS20650 phosphoenolpyruvate--protein phosphotransferase

Query= reanno::psRCH2:GFF3291
         (960 letters)



>FitnessBrowser__Koxy:BWI76_RS20650
          Length = 575

 Score =  395 bits (1016), Expect = e-114
 Identities = 239/572 (41%), Positives = 341/572 (59%), Gaps = 27/572 (4%)

Query: 408 VNGIAASPGIAIGPVLVRKPQ--VIDYPKRGESPVI-ELQRL-------DAALDKVHADI 457
           ++GI ASPGIA G  L+ K    VID  K     V  E++R         A L+ +    
Sbjct: 2   ISGILASPGIAFGKALLLKEDEIVIDRKKISADKVDQEVERFLNGRTKASAQLEVIKTKA 61

Query: 458 GTLIDESQVASIRDIFTTHQAMLKDPALREEVQVRLQ-KGLSAEAAWMEEIESAAQQQEA 516
           G    E + A    IF  H  +L+D  L +E+   ++ K ++A+AA  E IE  A   E 
Sbjct: 62  GETFGEEKEA----IFEGHIMLLEDEELEQEIIALIKDKHMTADAAANEVIEGQATALEE 117

Query: 517 LHDKLLAERAADLRDVGRRVLACLTG---VEAEQAPDEPYILVMDEVAPSDVATLNAQRV 573
           L D+ L ERAAD+RD+G+R+L  + G   ++     DE  ILV  ++ PS+ A LN  +V
Sbjct: 118 LDDEYLKERAADVRDIGKRLLRNILGLAIIDLSAIQDE-VILVAADLTPSETAQLNLNKV 176

Query: 574 AGILTAGGGATSHSAIIARALGIPAIVGAGPGVLGLARNTLLLLDGERGELLVAPSGAQL 633
            G +T  GG TSH++I+AR+L +PAIVG G     +     L+LD    ++LV PS  Q+
Sbjct: 177 LGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNGDYLVLDAVNNQVLVNPSDQQI 236

Query: 634 EQARSERA-AREERKHLANERRMDAAVTRDGHPVEIAANIGAAGETPEAVAMGAEGIGLL 692
           E+ R+ +A   EE+  LA  + + A +T DGH VE+ ANIG   +   A   GAEG+GL 
Sbjct: 237 EELRNLQAQVAEEKAELAKLKDLPA-ITLDGHQVEVCANIGTVRDVEGAERNGAEGVGLY 295

Query: 693 RTELVFMNHSQAPNQATQEAEYRRVLEALEGRPLVVRTLDVGGDKPLPYWPMPAEENPFL 752
           RTE +FM+    P +  Q A Y+ V EA   + ++VRT+D+GGDK LPY   P EENPFL
Sbjct: 296 RTEFLFMDRDSLPTEEEQFAAYKAVAEACGSQAVIVRTMDIGGDKELPYMNFPKEENPFL 355

Query: 753 GVRGIRLSLQRPDILETQLRALLASADGRPLRIMFPMVGNIDEWRTAKAMVDRLRVELP- 811
           G R +R+++ R +IL  Q+RA+L ++    LRIMFPM+ +++E R  K  ++  + EL  
Sbjct: 356 GWRAVRIAMDRKEILRDQVRAILRASAFGKLRIMFPMIISVEEVRALKKEIEIYKQELRD 415

Query: 812 -----VADLQVGIMIEIPSAALIAPVLAQEVDFFSIGTNDLTQYTLAIDRGHPTLSGQAD 866
                   +++G+M+E P+AA IA  LA+EVDFFSIGTNDLTQYTLA+DRG+  +S    
Sbjct: 416 EGKAFDESIEIGVMVETPAAATIARHLAKEVDFFSIGTNDLTQYTLAVDRGNDMISHLYQ 475

Query: 867 GLHPAVLRLIGMTVEAAHAHGKWVGVCGELAADALAVPLLVGLGVDELSVSARSIALVKA 926
            + P+VL LI   ++A+HA GKW G+CGELA D  A  LL+G+G+DE S+SA SI  +K 
Sbjct: 476 PMSPSVLTLIKQVIDASHAEGKWTGMCGELAGDERATLLLLGMGLDEFSMSAISIPRIKK 535

Query: 927 RVRELDFAACQRLAQQALMLPGAHEVRAFVGE 958
            +R  +F   + LA+QAL  P   E+   V +
Sbjct: 536 IIRNTNFEDAKVLAEQALAQPTTDELMTLVNK 567


Lambda     K      H
   0.317    0.134    0.384 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 981
Number of extensions: 37
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 960
Length of database: 575
Length adjustment: 40
Effective length of query: 920
Effective length of database: 535
Effective search space:   492200
Effective search space used:   492200
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources