Alignments for a candidate for HSERO_RS05250 in Klebsiella michiganensis M5al

GapMind for catabolism of small carbon sources

Alignments for a candidate for HSERO_RS05250 in Klebsiella michiganensis M5al

Align Ribose import ATP-binding protein RbsA; EC 7.5.2.7 (characterized, see rationale)
to candidate BWI76_RS07240 BWI76_RS07240 D-ribose transporter ATP-binding protein

Query= uniprot:D8J111
         (520 letters)



>FitnessBrowser__Koxy:BWI76_RS07240
          Length = 494

 Score =  443 bits (1139), Expect = e-129
 Identities = 237/487 (48%), Positives = 331/487 (67%), Gaps = 6/487 (1%)

Query: 28  VCKRFPGVLALDNCQFELAAGEVHALMGENGAGKSTLMKILSGVYQRDSGDILLDGKPVE 87
           + K FPGV ALDN    +  G VHALMGENGAGKSTLMK L G+Y+ D G I + G+PV+
Sbjct: 11  ISKFFPGVKALDNVSLRVRPGTVHALMGENGAGKSTLMKCLIGIYRPDKGAIRVKGEPVQ 70

Query: 88  ITEPRQAQALGIGIIHQELNLMNHLSAAQNIFIGREPRKAMGLFIDEDELNRQAAAIFAR 147
             +   A   GI +IHQELNL+ H++ A+NI++GREP K    F+D  +L RQ   +  +
Sbjct: 71  FQDTMDALRSGISMIHQELNLVPHMTVAENIWLGREPMKYG--FVDHRQLARQTQDLLDK 128

Query: 148 MRLDMDPSTPVGELTVARQQMVEIAKALSFDSRVLIMDEPTAALNNAEIAELFRIIRDLQ 207
           + + +     VGEL++A QQMVEIAKA+S+++ ++IMDEPT+AL  +E+A LF IIRDL+
Sbjct: 129 LNIRLSADRLVGELSIASQQMVEIAKAVSWNADIVIMDEPTSALTESEVAHLFTIIRDLR 188

Query: 208 AQGVGIVYISHKMDELRQIADRVSVMRDGKYIATVPMQETSMDTIISMMVGRALDGEQRI 267
            QG  I+YISHKMDE+  I D +SV RDG ++ +    E +  ++I+ MVGR L   Q  
Sbjct: 189 QQGKAIIYISHKMDEIFAITDEISVFRDGTWVGSKQTTEFTRQSLITQMVGRELT--QLF 246

Query: 268 PP-DTSRNDVVLEVRGLNRGRAIRDVSFTLRKGEILGFAGLMGAGRTEVARAIFGADPLE 326
           P  + +  + VL VR L+R  A  D++F++R+GEILG AGL+GAGR+EV  ++FG +  +
Sbjct: 247 PKFNNAIGEEVLTVRNLSRKGAFHDINFSVRRGEILGVAGLVGAGRSEVMESLFGMEKAD 306

Query: 327 AGEIIIHGGKAVIKSPADAVAHGIGYLSEDRKHFGLAVGMDVQANIALSSMGRFT-RVGF 385
           +GE++I G    I SP+ A+  G+  L+EDRK  GL + + V  N+++  M  +  + GF
Sbjct: 307 SGEVLIDGMPVNIDSPSTAIEKGMALLTEDRKKSGLFLVLSVLENMSIVKMPEYIGKTGF 366

Query: 386 MDQRAIREAAQMYVRQLAIKTPSVEQQARLLSGGNQQKIVIAKWLLRDCDILFFDEPTRG 445
           +    + E     +R+L IKTP+++Q    LSGGNQQK++IA+WLL    IL  DEPTRG
Sbjct: 367 VQHLKMAEDCMEQIRRLNIKTPTMDQIINNLSGGNQQKVLIARWLLAQPKILILDEPTRG 426

Query: 446 IDVGAKSEIYKLLDALAEQGKAIVMISSELPEVLRMSHRVLVMCEGRITGELARADATQE 505
           IDVGAK+EIY L+  LA +G A++M+SSELPE+L MS RV+VM EGRITG L + DA QE
Sbjct: 427 IDVGAKAEIYHLISELANRGVAVIMVSSELPEILGMSDRVMVMHEGRITGILDKEDADQE 486

Query: 506 KIMQLAT 512
            I+ LA+
Sbjct: 487 TILSLAS 493



 Score = 85.9 bits (211), Expect = 3e-21
 Identities = 63/246 (25%), Positives = 116/246 (47%), Gaps = 10/246 (4%)

Query: 274 NDVVLEVRGLNRG----RAIRDVSFTLRKGEILGFAGLMGAGRTEVARAIFGADPLEAGE 329
           N   LE  G+++     +A+ +VS  +R G +    G  GAG++ + + + G    + G 
Sbjct: 2   NAFALEAEGISKFFPGVKALDNVSLRVRPGTVHALMGENGAGKSTLMKCLIGIYRPDKGA 61

Query: 330 IIIHGGKAVIKSPADAVAHGIGYLSEDRKHFGLAVGMDVQANIALSSMGRFTRVGFMDQR 389
           I + G     +   DA+  GI  + ++     L   M V  NI L       + GF+D R
Sbjct: 62  IRVKGEPVQFQDTMDALRSGISMIHQE---LNLVPHMTVAENIWLGREPM--KYGFVDHR 116

Query: 390 AIREAAQMYVRQLAIKTPSVEQQARLLSGGNQQKIVIAKWLLRDCDILFFDEPTRGIDVG 449
            +    Q  + +L I+  S ++    LS  +QQ + IAK +  + DI+  DEPT  +   
Sbjct: 117 QLARQTQDLLDKLNIRL-SADRLVGELSIASQQMVEIAKAVSWNADIVIMDEPTSALTES 175

Query: 450 AKSEIYKLLDALAEQGKAIVMISSELPEVLRMSHRVLVMCEGRITGELARADATQEKIMQ 509
             + ++ ++  L +QGKAI+ IS ++ E+  ++  + V  +G   G     + T++ ++ 
Sbjct: 176 EVAHLFTIIRDLRQQGKAIIYISHKMDEIFAITDEISVFRDGTWVGSKQTTEFTRQSLIT 235

Query: 510 LATQRE 515
               RE
Sbjct: 236 QMVGRE 241


Lambda     K      H
   0.320    0.135    0.372 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 637
Number of extensions: 23
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 520
Length of database: 494
Length adjustment: 34
Effective length of query: 486
Effective length of database: 460
Effective search space:   223560
Effective search space used:   223560
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
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Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources