Align D-galactonate dehydratase; EC 4.2.1.6 (characterized)
to candidate BWI76_RS27935 BWI76_RS27935 galactonate dehydratase
Query= CharProtDB::CH_024133 (382 letters) >FitnessBrowser__Koxy:BWI76_RS27935 Length = 382 Score = 734 bits (1895), Expect = 0.0 Identities = 355/382 (92%), Positives = 370/382 (96%) Query: 1 MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAAVHELGDYLIGQDPSRI 60 MKITK+TTYRLPPRWMFLKI+TDEGVVGWGEPVIEGRARTVEAAVHELGDYLIGQDP+RI Sbjct: 1 MKITKLTTYRLPPRWMFLKIDTDEGVVGWGEPVIEGRARTVEAAVHELGDYLIGQDPARI 60 Query: 61 NDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPVWQLMGGLVRDKIKAYSWV 120 NDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPVWQLMGGLVRDKIKAYSWV Sbjct: 61 NDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPVWQLMGGLVRDKIKAYSWV 120 Query: 121 GGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAVDAAVNTVAQIREAFGNQIEF 180 GGDRPA+VIDGIK LR IGFDTFKLNGCEELGLIDNSRAVDAAVNTVAQIREAFGN+IEF Sbjct: 121 GGDRPAEVIDGIKKLRGIGFDTFKLNGCEELGLIDNSRAVDAAVNTVAQIREAFGNEIEF 180 Query: 181 GLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAEYYPKLAAQTHIPLAAGERMFSR 240 GLDFHGRVSAPMAKVLIKELE YRPLFIEEPVLAEQAEYYP+LAAQT IP+AAGERMFSR Sbjct: 181 GLDFHGRVSAPMAKVLIKELEQYRPLFIEEPVLAEQAEYYPRLAAQTSIPIAAGERMFSR 240 Query: 241 FDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAEAYDVTLAPHCPLGPIALAACLHID 300 F+FKRVLEAGG++ILQPDLSHAGGITECYKIAGMAEAYDV LAPHCPLGPIALAACLH+D Sbjct: 241 FEFKRVLEAGGLAILQPDLSHAGGITECYKIAGMAEAYDVALAPHCPLGPIALAACLHVD 300 Query: 301 FVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFSMVGGFFKPLTKPGLGVEIDEAKVIEF 360 FVSYNAV QEQSMGIHYNKGAELLDFVKNKEDF+M GGFFKPL KPGLGVEIDEA+V+E Sbjct: 301 FVSYNAVFQEQSMGIHYNKGAELLDFVKNKEDFNMEGGFFKPLMKPGLGVEIDEARVMEL 360 Query: 361 SKNAPDWRNPLWRHEDNSVAEW 382 S+NAPDWRNPLWR ED VAEW Sbjct: 361 SQNAPDWRNPLWRLEDGVVAEW 382 Lambda K H 0.321 0.140 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 692 Number of extensions: 15 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 382 Length of database: 382 Length adjustment: 30 Effective length of query: 352 Effective length of database: 352 Effective search space: 123904 Effective search space used: 123904 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 50 (23.9 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory